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GM-CSF

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Recombinant Bovine GM-CSF

Product Type
Recombinant Protein
Specificity
GM-CSF

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PBP011
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Recombinant Bovine GM-CSF is a tissue culture supernatant containing active bovine GM-CSF, a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

This preparation has been shown to have biological activity in proliferation assays utilizing TF-1 cells. The specific concentration of GM-CSF has not been assayed.

Target Species
Bovine
Product Form
Tissue Culture Supernatant from HEK293T cells expressing recombinant bovine GM-CSF - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Source
HEK293T
Preservative Stabilisers
1% Bovine Serum Albumin
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 1/2 1/10
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

References for GM-CSF

  1. Iwan, M. et al. (2014) Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures.
    Bulletin of the Veterinary Institute in Pulawy. 58: 347-52.
  2. Iwan, E. et al. (2018) Determination of cytokine profiles in populations of dendritic cells from cattle infected with bovine leukaemia virus.
    Pol J Vet Sci. 21 (4): 681-90.

Synonyms
Granulocyte Macrophage Colony Stimulating Factor
UniProt
P11052
Entrez Gene
CSF2
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity

PBP011

1608 163460 164105

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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