GM-CSF

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Recombinant Bovine GM-CSF

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PBP011 FN 1 ml
Recombinant Bovine GM-CSF is a tissue culture supernatant containing active bovine GM-CSF, a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

This preparation has been shown to have biological activity in proliferation assays utilizing TF-1 cells. The specific concentration of GM-CSF has not been assayed.

Product Details

Target Species
Bovine
Product Form
Tissue Culture Supernatant from HEK293T cells expressing recombinant bovine GM-CSF - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preservative Stabilisers
None present

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P11052 Related reagents
Entrez Gene
CSF2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Regulatory
For research purposes only

Applications of GM-CSF

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 1/2 1/10
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.

Product Specific References

References for GM-CSF

  1. Iwan, M. et al. (2014) Application of in situ PCR for the Detection of Bovine Leukaemia Virus (BLV) Infection in Dendritic Cell cultures.
    Bulletin of the Veterinary Institute in Pulawy. 58: 347-52.