Collagen III

Product Details
- Target Species
- Bovine
- Product Form
- Purified Protein - lyophilized
- Reconstitution
- Reconstitute with 2.0 ml of 0.1M acetic acid, pH 3.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preparation
- Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by differential salt precipitation and purification by ion-exchange chromatography.
- Preservative Stabilisers
- None present
- Purity
- Non-collagenous proteins are less than 0.5%. Typical contaminants are cross-linked collagen type II dimers and trimers (approximately 10%). Other collagen types constitute less than 5% by immunoassay and analytical chromatography.
- Approx. Protein Concentrations
- Total protein concentration 5.0 mg/ml after reconstitution
- Protein Molecular Weight
- 300 kDa
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P04258
- Entrez Gene
- COL3A1
- GO Terms
- GO:0005578 proteinaceous extracellular matrix
- Regulatory
- For research purposes only
Applications of Collagen III
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
Product Specific References
References for Collagen III
-
Hitsumoto, Y. et al. (2013) Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin.
Anaerobe. pii: S1075-9964(13)00187-X.
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