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Collagen III

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Native Bovine Collagen III

Product Type
Purified Protein
Specificity
Collagen III

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Product Code Applications Pack Size List Price Your Price Qty
2150-0555
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 10 mg
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Purified collagen type III from bovine skin. Collagen type III occurs in most soft connective tissues. It is composed of trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.

Target Species
Bovine
Product Form
Purified Protein - lyophilized
Reconstitution
Reconstitute with 2.0 ml of 0.1M acetic acid, pH 3.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by differential salt precipitation and purification by ion-exchange chromatography.
Preservative Stabilisers
None present
Purity
Non-collagenous proteins are less than 0.5%. Typical contaminants are cross-linked collagen type II dimers and trimers (approximately 10%). Other collagen types constitute less than 5% by immunoassay and analytical chromatography.
Approx. Protein Concentrations
Total protein concentration 5.0 mg/ml after reconstitution
Protein Molecular Weight
300 kDa
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Collagen III

  1. Hitsumoto, Y. et al. (2013) Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin.
    Anaerobe. pii: S1075-9964(13)00187-X.

UniProt
P04258
Entrez Gene
COL3A1
GO Terms
GO:0005578 proteinaceous extracellular matrix
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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