Native Bovine Collagen III
- Product Type
- Purified Protein
- Collagen III
|Purified collagen type III from bovine skin. Collagen type III occurs in most soft connective tissues. It is composed of trimers of identical alpha 1(III) chains. The chains are linked to each other by interchain disulfide bonds. Trimers are also cross-linked via hydroxylysines.|
- Target Species
- Product Form
- Purified Protein - lyophilized
- Reconstitute with 2.0 ml of 0.1M acetic acid, pH 3.0. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by differential salt precipitation and purification by ion-exchange chromatography.
- Preservative Stabilisers
- None present
- Non-collagenous proteins are less than 0.5%. Typical contaminants are cross-linked collagen type II dimers and trimers (approximately 10%). Other collagen types constitute less than 5% by immunoassay and analytical chromatography.
- Approx. Protein Concentrations
- Total protein concentration 5.0 mg/ml after reconstitution
- Protein Molecular Weight
- 300 kDa
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
References for Collagen III
Hitsumoto, Y. et al. (2013) Adhesive properties of Clostridium perfringens to extracellular matrix proteins collagens and fibronectin.
Anaerobe. pii: S1075-9964(13)00187-X.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Bovine Products
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