Collagen I
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Native Bovine Collagen I
- Product Type
- Purified Protein
- Specificity
- Collagen I
Antibody Quality and Performance Guarantee
| Collagen type I 90% Collagen type III 10% Non-collagenous proteins <0.5% |
- Target Species
- Bovine
- Product Form
- Purified protein from calf tendon - lyophilized
- Reconstitution
- Use 0.5M acetic acid, pH 3.0. Dispersed collagen have to be thoroughly homogenized in a Potter after addition of the solute and left overnight in the coldroom to dissolve.
Dissolved collagen retains immunologic properties of native collagen type I. Thermal denaturation (5 minutes at 100ºC) converts dissolved collagen to gelatin. - Preparation
- Bovine collagen type I was isolated from calf tendons. Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by multiple steps of differential salt precipitation and ion-exchange chromatography.
- Buffer Solution
- Essentially salt free
- Preservative Stabilisers
- None present
- Protein Molecular Weight
- ~300 kDa
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- UniProt
- P02465
- P02453
- Entrez Gene
- COL1A1
- COL1A2
- GO Terms
- GO:0005201 extracellular matrix structural constituent
2150-0515
150826
157466
160443
165247
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with COLLAGEN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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