CD71 antibody | YTA74.4
Rat anti Mouse CD71:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- YTA74.4
- Isotype
- IgG2a
- Specificity
- CD71
Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron. Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Concanavilin A activated mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from an immunized DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Rat IgG2a Negative Control:Alexa Fluor® 647 | MCA1212A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
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Description | Rat IgG2a Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
List Price | Your Price | ||||||
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Description | Mouse Seroblock FcR |
References for CD71 antibody
-
Millot, S. et al. (2010) Erythropoietin stimulates spleen BMP4-dependent stress erythropoiesis and partially corrects anemia in a mouse model of generalized inflammation.
Blood. 116: 6072-81. -
Kuo, Y.M. et al. (2004) Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse.
Gut. 53: 201-6. -
Krysiak, K. et al. (2015) Reduced levels of Hspa9 attenuate Stat5 activation in mouse B cells.
Exp Hematol. 43 (4): 319-30.e10. -
Byun, M. et al. (2007) Cowpox virus exploits the endoplasmic reticulum retention pathway to inhibit MHC class I transport to the cell surface.
Cell Host Microbe. 2: 306-15. -
Ripich, T. and Jessberger, R. (2011) SWAP-70 regulates erythropoiesis by controlling α4 integrin.
Haematologica. 96: 1743-52. -
Hadziahmetovic, M. et al. (2012) Microarray analysis of murine retinal light damage reveals changes in iron regulatory, complement, and antioxidant genes in the neurosensory retina and isolated RPE.
Invest Ophthalmol Vis Sci. 53 (9): 5231-41. -
Niewoehner, J. et al. (2014) Increased brain penetration and potency of a therapeutic antibody using a monovalent molecular shuttle.
Neuron. 81: 49-60. -
Sands, S.A. et al. (2015) The habenula and iron metabolism in cerebral mouse models of multiple sclerosis.
Neurosci Lett. 606: 204-8.
View The Latest Product References
-
Baumann, B. et al. (2017) Conditional Müller Cell Ablation Leads to Retinal Iron Accumulation.
Invest Ophthalmol Vis Sci. 58 (10): 4223-34. -
Nelvagal, H.R. et al. (2020) Comparative proteomic profiling reveals mechanisms for early spinal cord vulnerability in CLN1 disease.
Sci Rep. 10 (1): 15157. -
Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
J Appl Toxicol. 41 (12): 1952-65. -
Zhang, K.R. et al. (2022) Conditional knockout of hephaestin in the neural retina disrupts retinal iron homeostasis.
Exp Eye Res. 218: 109028. -
Hargreaves, A. et al. (2022) Tumours modulate the systemic vascular response to anti-angiogenic therapy.
J Appl Toxicol. 42 (8): 1371-84. -
Hargreaves, A. et al. (2021) Tumors modulate fenestrated vascular beds and host endocrine status.
J Appl Toxicol. 41 (12): 1952-65.
Further Reading
-
Lesley, J. et al. (1984) Expression of transferrin receptor on murine hematopoietic progenitors.
Cell Immunol. 83 (1): 14-25. -
Trowbridge, I.S. et al. (1982) Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody.
J Cell Physiol. 112 (3): 403-10.
- Synonyms
- Transferrin Receptor
- RRID
- AB_324926
- UniProt
- Q62351
- Entrez Gene
- Tfrc
- GO Terms
- GO:0005886 plasma membrane
- GO:0008233 peptidase activity
- GO:0005381 iron ion transmembrane transporter activity
- GO:0016021 integral to membrane
- GO:0004998 transferrin receptor activity
- GO:0005768 endosome
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- View More GO Terms
- GO:0042470 melanosome
- GO:0048471 perinuclear region of cytoplasm
- GO:0070062 extracellular vesicular exosome
MCA1033A647
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