IgG antibody
Goat anti Rabbit IgG antibody (403001) used as a capture antibody in a sandwich ELISA for the evaluation of total rabbit IgG in serum and vaginal lavage samples.
Image caption:
PRO 2000 co-application induces increased systemic and mucosal antibody responses in rabbits.
New Zealand White rabbits (n = 6) received six vaginal immunizations using 50 mg antigen per dose in the presence or absence of 1% PRO 2000. (A) Time course of rabbit serum IgG endpoint titres. (B) Endpoint IgG titres of week 12 vaginal lavage samples. Red symbols represent data from animals immunized with gp140 + PRO 2000. Pre-immune indicates samples collected prior to the first immunization; gp140 + PRO indicates combined gp140 and PRO 2000. Titre data were log-transformed and are shown as mean ± SEM. The grey dashed line indicates the ELISA detection limit. * p<0.05; ** p<0.01.
From: Wegmann F, Krashias G, Lühn K, Laamanen K, Vieira S, Jeffs SA, et al. (2011)
A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment.
PLoS ONE 6(1): e15861.
Alkaline phosphatase conjugated Goat anti Rabbit IgG antibody (403004) used as a tertiary detection regent for the evaluation of SARS CoV2 Nucleoprotein levels by ELISA.
Image caption:
Representative calibration curve for quantification of N protein by developed prototype ELISA.
From: Mladenovic Stokanic M, Simovic A, Jovanovic V, Radomirovic M, Udovicki B, Krstic Ristivojevic M, Djukic T, Vasovic T, Acimovic J, Sabljic L, Lukic I, Kovacevic A, Cujic D, Gnjatovic M, Smiljanic K, Stojadinovic M, Radosavljevic J, Stanic-Vucinic D, Stojanovic M, Rajkovic A, Cirkovic Velickovic T.
Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species.
Int J Mol Sci. 2023 Dec 26;25(1):333.
doi: 10.3390/ijms25010333.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Rabbit IgG:Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| Goat anti Rabbit IgG antibody recognizes the heavy chain of rabbit IgG. Cross-reactivity against rabbit IgM is reduced by solid phase adsorption. This antibody may react with IgG from other species. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Antiserum Preparation
- Antisera to rabbit IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Purified rabbit IgG from normal rabbit serum
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/4000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
PLoS One. 6(1):e15861. -
Radomirovic, M. et al. (2022) Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating
Food Hydrocolloids. 123: 107169. -
Mladenovic Stokanic, M. et al. (2023) Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species
Int J Mol Sci. 25 (1): 333.
- RRID
- AB_609698
- UniProt
- P01870
- Entrez Gene
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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