Cytosine Deaminase antibody

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/500	1/2500
Western Blotting		1/100	1/500

Western Blotting

In Western blot two bands were visualised by ECL at 13kD and 17kD.

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References for Cytosine Deaminase antibody

1. Fuerer, C. et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.
   Gene Ther. 11: 142-51
2. Hammer, K. et al. (2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.
   Int J Cancer. 137 (4): 978-90.
3. Amon, A. et al. (2008) Methods and Composition for Diagnosing and Treating Cancer
   US patent application US 11/767,200
4. Pan, X. et al. (2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.
   Pancreas. 26: 274-8.
5. Conrad, C. et al. (2005) _Δ24-hyCD adenovirus suppresses glioma growth in vivo by combining oncolysis and chemosensitization.
   Cancer Gene Ther. 12: 284-94.
6. Michnick, S.W. et al. (2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.
   Methods Enzymol. 470: 335-68.
7. Komissarov, A. et al. (2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.
   Mol Biol Rep. 44 (4): 323-32.
8. Hanauer, J.R.H. et al. (2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.
   Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].
View The Latest Product References

RRID

AB_618795

UniProt

Q12178

GO Terms

GO:0004131 cytosine deaminase activity

GO:0005634 nucleus

GO:0005737 cytoplasm

GO:0008270 zinc ion binding

GO:0042802 identical protein binding

GO:0008655 pyrimidine-containing compound salvage

GO:0019858 cytosine metabolic process

GO:0046087 cytidine metabolic process