Cytosine Deaminase antibody
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Sheep anti cytosine deaminase antibody recognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004).
Product Details
- Target Species
- Yeast
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antisera to cytosine deaminase were raised by repeated immunisations sheep of with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified yeast cytosine deaminase.
- Approx. Protein Concentrations
- Ig concentration 5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q12178
- GO Terms
- GO:0004131 cytosine deaminase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0008655 pyrimidine-containing compound salvage
- GO:0019858 cytosine metabolic process
- GO:0046087 cytidine metabolic process
- Regulatory
- For research purposes only
Applications of Cytosine Deaminase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/500 | 1/2500 | |
| Western Blotting | 1/100 | 1/500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- In Western blot two bands were visualised by ECL at 13kD and 17kD.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Cytosine Deaminase antibody
-
Fuerer, C. et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.
Gene Ther. 11: 142-51 -
Hammer, K. et al. (2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.
Int J Cancer. 137 (4): 978-90. -
Amon, A. et al. (2008) Methods and Composition for Diagnosing and Treating Cancer
US patent application US 11/767,200 -
Pan, X. et al. (2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.
Pancreas. 26: 274-8. -
Conrad, C. et al. (2005) Δ24-hyCD adenovirus suppresses glioma growth in vivo by combining oncolysis and chemosensitization.
Cancer Gene Ther. 12: 284-94. -
Michnick, S.W. et al. (2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.
Methods Enzymol. 470: 335-68. -
Komissarov, A. et al. (2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.
Mol Biol Rep. 44 (4): 323-32. -
Hanauer, J.R.H. et al. (2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.
Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].
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