Sheep anti cytosine deaminase antibody
recognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004
- Target Species
- Product Form
- Purified Ig - liquid
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antisera to cytosine deaminase were raised by repeated immunisations sheep of with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Purified yeast cytosine deaminase.
- Approx. Protein Concentrations
- Ig concentration 5mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- GO Terms
cytosine deaminase activity
zinc ion binding
identical protein binding
pyrimidine-containing compound salvage
cytosine metabolic process
cytidine metabolic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Cytosine Deaminase antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- In Western blot two bands were visualised by ECL at 13kD and 17kD.
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Secondary Antibodies Available
Product Specific References
References for Cytosine Deaminase antibody
Fuerer, C. et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.
Gene Ther. 11: 142-51
Hammer, K. et al. (2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.
Int J Cancer. 137 (4): 978-90.
Amon, A. et al. (2008) Methods and Composition for Diagnosing and Treating Cancer
US patent application US 11/767,200
Pan, X. et al. (2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.
Pancreas. 26: 274-8.
Conrad, C. et al. (2005) Δ24-hyCD adenovirus suppresses glioma growth in vivo by combining oncolysis and chemosensitization.
Cancer Gene Ther. 12: 284-94.
Michnick, S.W. et al. (2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.
Methods Enzymol. 470: 335-68.
Komissarov, A. et al. (2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.
Mol Biol Rep. 44 (4): 323-32.
Hanauer, J.R.H. et al. (2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.
Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].
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