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Cytosine Deaminase antibody

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Sheep anti Yeast Cytosine Deaminase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
2485-4906
Datasheet
E WB
SDS
1 ml

Sheep anti cytosine deaminase antibody recognizes cytosine deaminase, also known as cytosine aminohydrolase. Cytosine deaminase is a 158 amino acid ~18 kDa enzyme involved in the conversion of cytosine to uracil. Sheep anti Human cytosine deaminase antibody recognizes the native antigen in ELISA and has been successfully used for detection of the enzyme by western blotting (Fuerer and Iggo 2004).

Product Details

Target Species
Yeast
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Antiserum Preparation
Antisera to cytosine deaminase were raised by repeated immunisations sheep of with highly purified antigen.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified yeast cytosine deaminase.
Approx. Protein Concentrations
Ig concentration 5mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
Q12178
GO Terms
GO:0004131 cytosine deaminase activity
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0008270 zinc ion binding
GO:0042802 identical protein binding
GO:0008655 pyrimidine-containing compound salvage
GO:0019858 cytosine metabolic process
GO:0046087 cytidine metabolic process
Regulatory
For research purposes only

Applications of Cytosine Deaminase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/2500
Western Blotting 1/100 1/500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
In Western blot two bands were visualised by ECL at 13kD and 17kD.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for Cytosine Deaminase antibody

  1. Fuerer, C. et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.
    Gene Ther. 11: 142-51
  2. Hammer, K. et al. (2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.
    Int J Cancer. 137 (4): 978-90.
  3. Amon, A. et al. (2008) Methods and Composition for Diagnosing and Treating Cancer
    US patent application US 11/767,200
  4. Pan, X. et al. (2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.
    Pancreas. 26: 274-8.
  5. Conrad, C. et al. (2005) Δ24-hyCD adenovirus suppresses glioma growth in vivo by combining oncolysis and chemosensitization.
    Cancer Gene Ther. 12: 284-94.
  6. Michnick, S.W. et al. (2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.
    Methods Enzymol. 470: 335-68.
  7. Komissarov, A. et al. (2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.
    Mol Biol Rep. 44 (4): 323-32.
  8. Hanauer, J.R.H. et al. (2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.
    Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].

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