Cytosine Deaminase antibody

Product Details
- Target Species
- Yeast
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography on Protein G
- Antiserum Preparation
- Antisera to cytosine deaminase were raised by repeated immunisations sheep of with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Purified yeast cytosine deaminase.
- Approx. Protein Concentrations
- Ig concentration 5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q12178
- GO Terms
- GO:0004131 cytosine deaminase activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0008270 zinc ion binding
- GO:0042802 identical protein binding
- GO:0008655 pyrimidine-containing compound salvage
- GO:0019858 cytosine metabolic process
- GO:0046087 cytidine metabolic process
- Regulatory
- For research purposes only
Applications of Cytosine Deaminase antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/500 | 1/2500 | |
Western Blotting | 1/100 | 1/500 |
- Western Blotting
- In Western blot two bands were visualised by ECL at 13kD and 17kD.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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Donkey anti Sheep/Goat IgG:FITC | STAR88F | C | 1 mg |
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Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Product Specific References
References for Cytosine Deaminase antibody
-
Fuerer, C. et al. (2004) 5-Fluorocytosine increases the toxicity of Wnt-targeting replicating adenoviruses that express cytosine deaminase as a late gene.
Gene Ther. 11: 142-51 -
Hammer, K. et al. (2015) Engineered adenoviruses combine enhanced oncolysis with improved virus production by mesenchymal stromal carrier cells.
Int J Cancer. 137 (4): 978-90. -
Amon, A. et al. (2008) Methods and Composition for Diagnosing and Treating Cancer
US patent application US 11/767,200 -
Pan, X. et al. (2003) Adenovirus-mediated gene transfer in the treatment of pancreatic cancer.
Pancreas. 26: 274-8. -
Conrad, C. et al. (2005) Δ24-hyCD adenovirus suppresses glioma growth in vivo by combining oncolysis and chemosensitization.
Cancer Gene Ther. 12: 284-94. -
Michnick, S.W. et al. (2010) A toolkit of protein-fragment complementation assays for studying and dissecting large-scale and dynamic protein-protein interactions in living cells.
Methods Enzymol. 470: 335-68. -
Komissarov, A. et al. (2017) Cytotoxic effect of co-expression of human hepatitis A virus 3C protease and bifunctional suicide protein FCU1 genes in a bicistronic vector.
Mol Biol Rep. 44 (4): 323-32. -
Hanauer, J.R.H. et al. (2019) High-affinity DARPin allows targeting of measles virus to glioblastoma multiforme in combination with protease targeting without loss of potency.
Molecular Therapy - Oncolytics. 14. Oct [Epub ahead of print].
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