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IgM antibody

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Anti Sheep IgM Antibody thumbnail image 1
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FITC conjugated Rabbit anti Sheep IgM antibody (AHP950F) used to detect IgM antibodies in serum by immunofluorescence.
Image caption:
Anti-pig antibody staining- Detection of anti-pig antibodies in sheep serum. a. Sheep serum taken preoperatively showed no evidence of anti-pig antibodies and as such there is no green or red fluorescence. The blue is auto-fluorescence of decellularized porcine valve with the addition of DAPI. b. Sheep serum taken at 1 week postoperatively shows an abundance of anti-pig IgM antibodies (green) and evidence of anti-pig IgG antibodies (red). The autoflorescence of decellularized porcine valve with the addition of DAPI (blue) is also seen. c-d. Sheep serum taken at 1 month and 2 months postoperatively shows an abundance of anti-pig IgM (green) and IgG (red) antibodies. e-h. Sheep serum at each time point was incubated with decellularized ovine valves. There is no evidence on any IgM (green) or IgG (red) antibodies. The addition of DAPI appears as autoflourescene of decellularized ovine valves. This was a negative control to show that antibody binding was specific to porcine tissue

From: Helder MRK, Stoyles NJ, Tefft BJ, Hennessy RS, Hennessy RRC, Dyer R, Witt T, Simari RD, Lerman A.
Xenoantigenicity of porcine decellularized valves.
J Cardiothorac Surg. 2017 Jul 17;12(1):56.

Rabbit anti Sheep IgM:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

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Product Code Applications Pack Size List Price Your Price Qty
AHP950P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
loader

Rabbit anti sheep IgM antibody recognizes ovine immunoglobulin M.

The antibody has been shown to react specifically with ovine IgM by immunoelectrophoresis and ELISA.

The antibody may cross react with IgM from other species.

Target Species
Sheep
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to ovine IgM were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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TMB Core BUF056A E 100 ml
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TMB Core+ BUF062A E 100 ml
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References for IgM antibody

  1. Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
    Res Vet Sci. 89 (2): 184-90.
  2. Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
    Vet Microbiol. 147 (3-4): 376-82.
  3. Marques, P.X. et al. (2011) Amniotic and allantoic fluids from experimentally infected sheep contain immunoglobulin specific for Chlamydophila abortus.
    Vet Immunol Immunopathol. 140: 1-9.
  4. Verhelst, D. et al. (2015) Interferon-gamma expression and infectivity of Toxoplasma infected tissues in experimentally infected sheep in comparison with pigs.
    Vet Parasitol. 207 (1-2): 7-16.
  5. Helder, M.R.K. et al. (2017) Xenoantigenicity of porcine decellularized valves.
    J Cardiothorac Surg. 12 (1): 56.

RRID
AB_567058

AHP950P

145990 155239 155308 165858

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