IgM antibody
FITC conjugated Rabbit anti Sheep IgM antibody (AHP950F) used to detect IgM antibodies in serum by immunofluorescence.
Image caption:
Anti-pig antibody staining- Detection of anti-pig antibodies in sheep serum. a. Sheep serum taken preoperatively showed no evidence of anti-pig antibodies and as such there is no green or red fluorescence. The blue is auto-fluorescence of decellularized porcine valve with the addition of DAPI. b. Sheep serum taken at 1 week postoperatively shows an abundance of anti-pig IgM antibodies (green) and evidence of anti-pig IgG antibodies (red). The autoflorescence of decellularized porcine valve with the addition of DAPI (blue) is also seen. c-d. Sheep serum taken at 1 month and 2 months postoperatively shows an abundance of anti-pig IgM (green) and IgG (red) antibodies. e-h. Sheep serum at each time point was incubated with decellularized ovine valves. There is no evidence on any IgM (green) or IgG (red) antibodies. The addition of DAPI appears as autoflourescene of decellularized ovine valves. This was a negative control to show that antibody binding was specific to porcine tissue
From: Helder MRK, Stoyles NJ, Tefft BJ, Hennessy RS, Hennessy RRC, Dyer R, Witt T, Simari RD, Lerman A.
Xenoantigenicity of porcine decellularized valves.
J Cardiothorac Surg. 2017 Jul 17;12(1):56.
Rabbit anti Sheep IgM:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Rabbit anti sheep IgM antibody recognizes ovine immunoglobulin M. The antibody has been shown to react specifically with ovine IgM by immunoelectrophoresis and ELISA. The antibody may cross react with IgM from other species. |
- Target Species
- Sheep
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to ovine IgM were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.2% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/200 | 1/2000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgM antibody
-
Hine, B.C. et al. (2010) Selective transport of IgE into ovine mammary secretions.
Res Vet Sci. 89 (2): 184-90. -
Vande Walle, K. et al. (2011) Rectal inoculation of sheep with E. coli O157:H7 results in persistent infection in the absence of a protective immune response.
Vet Microbiol. 147 (3-4): 376-82. -
Marques, P.X. et al. (2011) Amniotic and allantoic fluids from experimentally infected sheep contain immunoglobulin specific for Chlamydophila abortus.
Vet Immunol Immunopathol. 140: 1-9. -
Verhelst, D. et al. (2015) Interferon-gamma expression and infectivity of Toxoplasma infected tissues in experimentally infected sheep in comparison with pigs.
Vet Parasitol. 207 (1-2): 7-16. -
Helder, M.R.K. et al. (2017) Xenoantigenicity of porcine decellularized valves.
J Cardiothorac Surg. 12 (1): 56.
- RRID
- AB_567060
View more products with IgM specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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