Tyrosine Hydroxylase antibody
Sheep anti Rat tyrosine hydroxylase antibody recognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mammals Expected from Sequence Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to tyrosine hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by immunoaffinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol - Immunogen
- Rat Tyrosine hydroxylase purified from pheochromocytoma.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04177
- Entrez Gene
- Th
- GO Terms
- GO:0042136 neurotransmitter biosynthetic process
- GO:0001666 response to hypoxia
- GO:0005739 mitochondrion
- GO:0004511 tyrosine 3-monooxygenase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006585 dopamine biosynthetic process from tyrosine
- GO:0008021 synaptic vesicle
- GO:0008198 ferrous iron binding
- GO:0008199 ferric iron binding
- GO:0019825 oxygen binding
- GO:0016597 amino acid binding
- GO:0019717 synaptosome
- GO:0034617 tetrahydrobiopterin binding
- GO:0035240 dopamine binding
- GO:0043195 terminal button
- GO:0048545 response to steroid hormone stimulus
- GO:0051289 protein homotetramerization
- Regulatory
- For research purposes only
Applications of Tyrosine Hydroxylase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 1/1000 | ||
| Immunohistology - Frozen | 1/1000 | ||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1879 detects a band of approximately 60kDa in rat caudate cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Tyrosine Hydroxylase antibody
-
Xu, Z.Q. et al. (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies.
Neuroscience. 82 (3): 727-38. -
Olivas, A. et al. (2016) Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
J Neurosci. 36 (2): 479-88.
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