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Tyrosine Hydroxylase antibody

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Anti Rat Tyrosine Hydroxylase Antibody thumbnail image 1
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Western blot of rat caudate lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein using Sheep anti Rat tyrosine hydroxylase antibody (AHP1879).

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Sheep anti Rat Tyrosine Hydroxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Tyrosine Hydroxylase

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Product Code Applications Pack Size List Price Your Price Qty
AHP1879
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF WB 0.1 ml loader
List Price Your Price
loader

Sheep anti Rat tyrosine hydroxylase antibody recognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to tyrosine hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by immunoaffinity chromatography.
Buffer Solution
10mM HEPES pH7.5, 150mM NaCl
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
50% Glycerol
Immunogen
Rat Tyrosine hydroxylase purified from pheochromocytoma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/1000
Immunohistology - Frozen 1/1000
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1879 detects a band of approximately 60kDa in rat caudate cell lysates.

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References for Tyrosine Hydroxylase antibody

  1. Xu, Z.Q. et al. (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies.
    Neuroscience. 82 (3): 727-38.
  2. Olivas, A. et al. (2016) Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
    J Neurosci. 36 (2): 479-88.

RRID
AB_2201649
UniProt
P04177
Entrez Gene
Th
GO Terms
GO:0042136 neurotransmitter biosynthetic process
GO:0001666 response to hypoxia
GO:0005739 mitochondrion
GO:0004511 tyrosine 3-monooxygenase activity
GO:0005829 cytosol
GO:0055114 oxidation-reduction process
GO:0006585 dopamine biosynthetic process from tyrosine
GO:0008021 synaptic vesicle
GO:0008198 ferrous iron binding
GO:0008199 ferric iron binding
GO:0019825 oxygen binding
GO:0016597 amino acid binding
GO:0019717 synaptosome
GO:0034617 tetrahydrobiopterin binding
GO:0035240 dopamine binding
GO:0043195 terminal button
GO:0048545 response to steroid hormone stimulus
GO:0051289 protein homotetramerization

AHP1879

154675 170105

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