Tyrosine Hydroxylase antibody
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Sheep anti Rat Tyrosine Hydroxylase
- Product Type
- Polyclonal Antibody
- Polyclonal IgG
- Tyrosine Hydroxylase
|Sheep anti Rat tyrosine hydroxylase antibody recognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mammals Expected from Sequence Rabbit
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to tyrosine hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by immunoaffinity chromatography.
- Buffer Solution
- 10mM HEPES pH7.5, 150mM NaCl
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.01% Bovine Serum Albumin
- Rat Tyrosine hydroxylase purified from pheochromocytoma.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/1000|
- Western Blotting
- AHP1879 detects a band of approximately 60kDa in rat caudate cell lysates.
References for Tyrosine Hydroxylase antibody
Xu, Z.Q. et al. (1998) Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies.
Neuroscience. 82 (3): 727-38.
Olivas, A. et al. (2016) Myocardial Infarction Causes Transient Cholinergic Transdifferentiation of Cardiac Sympathetic Nerves via gp130.
J Neurosci. 36 (2): 479-88.
- Entrez Gene
- GO Terms
- GO:0042136 neurotransmitter biosynthetic process
- GO:0001666 response to hypoxia
- GO:0005739 mitochondrion
- GO:0004511 tyrosine 3-monooxygenase activity
- GO:0005829 cytosol
- GO:0055114 oxidation-reduction process
- GO:0006585 dopamine biosynthetic process from tyrosine
- GO:0008021 synaptic vesicle
- GO:0008198 ferrous iron binding
- View More GO Terms
- GO:0008199 ferric iron binding
- GO:0019825 oxygen binding
- GO:0016597 amino acid binding
- GO:0019717 synaptosome
- GO:0034617 tetrahydrobiopterin binding
- GO:0035240 dopamine binding
- GO:0043195 terminal button
- GO:0048545 response to steroid hormone stimulus
- GO:0051289 protein homotetramerization
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Rat Products
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