TGN38 antibody
Sheep anti Rat TGN38 antibody (AHP499) used for identification of the trans-golgi network in mouse embryonic fibroblasts by immunofluorescence.
Image caption:
Phosphorylated TBK1 is localized to the TGN in ISD-stimulated primary MEFs. a, Immunostaining of phosphorylated TBK1 (red) in cells treated with nocodazole and ISD. GM130 (a CGN protein, cyan) and TGN46 (a TGN protein, magenta) were co-stained. Scale bars, 10 μm. b-c, The mini-Golgi stacks indicated by the boxes in (a) were magnified. The cis and trans-regions of the mini-Golgi stacks were outlined with dashed line.
From: Mukai, K. et al. 2016.
Activation of STING required Palmtoylation oat the Golgi.
Nat Comms article 11932.
Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).
TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).
TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- serum diluted - liquid
- Antiserum Preparation
- Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Recombinant fusion protein corresponding to extracellular domain of TGN38.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P19814 Related reagents
- Entrez Gene
- Tgoln1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005768 endosome
- GO:0005794 Golgi apparatus
- GO:0006895 Golgi to endosome transport
- Regulatory
- For research purposes only
Applications of TGN38 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | |||
| Immunohistology - Frozen | 0.1ug/ml | 1ug/ml | |
| Western Blotting | 0.1ug/ml | 1ug/ml | |
| Immuno-electron Microscopy | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/100 | 1/200 | |
| Western Blotting | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- Fixation with methanol or methanol/acetone recommended.
- Immunohistology
- Fixation with methanol or methanol/acetone recommended.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
Product Specific References
References for TGN38 antibody
-
Vo, Y.P. et al. (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells.
Biochem Biophys Res Commun. 324: 1004-10. -
Prabhu, Y. et al. (2014) Defective Transport of the Obesity Mutant PC1/3 N222D Contributes to Loss of Function.
Endocrinology. 155: 2391-401. -
Ni-Komatsu, L. et al. (2008) Identification of quinolines that inhibit melanogenesis by altering tyrosinase family trafficking.
Mol Pharmacol. 74:1576-86. -
Mathews, P.M. et al. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.
J Biol Chem. 277: 5299-307. -
Phillips, S.E. et al. (2006) Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.
Mol Biol Cell. 17: 2498-512. -
Waugh, M.G. et al. (2011) Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
J Lipid Res. 52: 582-9. -
Farah,. C.A. et al. (2006) Tau interacts with Golgi membranes and mediates their association with microtubules.
Cell Motil Cytoskeleton. 63: 710-24. -
Hesse, D. et al. (2010) Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1.
Biochem Biophys Res Commun. 394: 896-903. -
Miranda-Saksena, M. et al. (2002) In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body.
J Virol. 76: 9934-51. -
Procino, G. et al. (2006) Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment.
Am J Physiol Renal Physiol. 290: F985-94. -
Han, L. et al. (2008) A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.
Mol Endocrinol. 22: 1935-49. -
Probst, O.C. et al. (2006) The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.
J Cell Sci. 119 (Pt 23): 4935-43. -
Wasmeier, C. et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.
J Cell Biol. 175: 271-81. -
Hou, J.C. et al. (2006) A specific dileucine motif is required for the GGA-dependent entry of newly synthesized insulin-responsive aminopeptidase into the insulin-responsive compartment.
J Biol Chem. 281: 33457-66. -
Fortin, M.E. et al. (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF.
Biol Reprod. 74: 2-12. -
Vasile, E. et al. (2006) IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.
Exp Cell Res. 312: 3132-41. -
Acquatella-Tran Van Ba, I. et al. (2011) Reg-1α is a new neuronal secreted factor that stimulates neurite outgrowth via the Exostosin tumor-like 3 (EXTL3) receptor.
J Biol Chem. 287: 4726-39. -
Watanabe, T. et al. (2012) A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.
J Histochem Cytochem. 60: 588-602. -
Yamamoto, H. et al. (2016) Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition.
Bone. 84: 120-30. -
Koga, D. et al. (2015) Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components.
J Histochem Cytochem. 63 (12): 968-79. -
Mukai, K. et al. (2016) Activation of STING requires palmitoylation at the Golgi.
Nat Commun. 7: 11932. -
Koga, D. et al. (2017) Changes in the three-dimensional ultrastructure of membranous organelles in male rat pituitary gonadotropes after castration.
Biomed Res. 38 (1): 1-18. -
Kusumi, S. et al. (2018) Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy.
Biomed Res. 39 (1): 21-25.
Further Reading
-
Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
Biochem J. 270: 97-102.
