TGN38 antibody
Sheep anti Rat TGN38 antibody (AHP499) used for identification of the trans-golgi network in mouse embryonic fibroblasts by immunofluorescence.
Image caption:
Phosphorylated TBK1 is localized to the TGN in ISD-stimulated primary MEFs. a, Immunostaining of phosphorylated TBK1 (red) in cells treated with nocodazole and ISD. GM130 (a CGN protein, cyan) and TGN46 (a TGN protein, magenta) were co-stained. Scale bars, 10 μm. b-c, The mini-Golgi stacks indicated by the boxes in (a) were magnified. The cis and trans-regions of the mini-Golgi stacks were outlined with dashed line.
From: Mukai, K. et al. 2016.
Activation of STING required Palmtoylation oat the Golgi.
Nat Comms article 11932.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Rat TGN38 antibody (AHP499G) used to demonstrate the Golgi apparatus in murine embryonic fibroblasts in vitro. by immunofluorescence.
Image caption:
STING activates TBK1 at TGN in the α-COP variants-expressing cells.
High resolution images of Fig. 2b. Images of p-TBK1 were taken in the same conditions and displayed with the same contrast levels. The α-COP variants and EGFP-STING expressing Sting-/- MEFs were fixed, permeabilized, and stained for TGN38 (a Golgi protein) and p-TBK1. The magnified image of the perinuclear region is shown in the lower leftbox. Scale bars, 10 μm. Fluorescence intensity profile along the white line is shown below.
From: Mukai K, Ogawa E, Uematsu R, Kuchitsu Y, Kiku F, Uemura T, Waguri S, Suzuki T, Dohmae N, Arai H, Shum AK, Taguchi T.
Homeostatic regulation of STING by retrograde membrane traffic to the ER.
Nat Commun. 2021 Jan 4;12(1):61.
doi: 10.1038/s41467-020-20234-9.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Rat TGN38 antibody (AHP499G) used to evaluate TGN38 expression in PC12 lysate fractions by western blotting.
Image caption:
Fractions were subjected to SDS-PAGE on 6 or 10% tricene gels and proteins transferred to blotting membranes. Blots were probed with antibodies to α-tubulin (1:5000), Rab3a (1:1000) and TGN38 (1:500)
From: Caswell PT, Dickens M. JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells. Mol Cell Biochem. 2018 Jul;444(1-2):1-13. doi: 10.1007/s11010-017-3222-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Rat TGN38 antibody (AHP499) used to demonstrate golgi network in mouse cerebellar Purkinje cells by immunofluorescence.
Image caption:
Deletion of GPHR has an effect on the Golgi apparatus organization and distribution in PCs. A, Triple immunofluorescence for GM130 (cis-Golgi; green), TGN38 (trans-Golgi; red) and parvalbumin (Parv; cyan) in PCs from GPHRF/+;GluD2-Cre and GPHRF/F;GluD2-Cre mice at P30, P45, and P60. Scale bar: 20 μm.
From: Sou YS, Kakuta S, Kamikubo Y, Niisato K, Sakurai T, Parajuli LK, Tanida I, Saito H, Suzuki N, Sakimura K, Maeda Y, Kinoshita T, Uchiyama Y, Koike M.
Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice.
eNeuro. 2019 May 29;6(3):ENEURO.0427-18.2019. doi: 10.1523/ENEURO.0427-18.2019.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Sheep anti Rat TGN38 antibody (AHP499G) used to label Golgi apparatus in rat motor neurons by immunofluorescence.
Image caption:
Correlative immunofluorescence and backscattered electron (BSE) images of a motor neuron in the rat spinal cord. (A) Immunofluorescence image of a semithin cryosection immunocytochemically stained with sheep polyclonal anti-TGN38 (green pseudocolor) and goat polyclonal anti-choline acetyltransferase (white pseudocolor) antibodies. Nuclei are also labeled using 4’,6-diamidino-2-phenylindole (blue pseudocolor). (B) BSE image of an ultrathin section cut from the semithin cryosection embedded in resin. The BSE micrograph is of the same area as that shown in (A). (C) The immunofluorescence image in (A) is overlapped with the BSE image in (B). The boxed area is enlarged and shown in (D). (D) The higher magnification image demonstrates that the fluorescence dyes that indicate the Golgi-associated protein (TGN38) have appropriately localized the Golgi apparatus. (E) The BSE image eliminating the fluorescence image from the merged image in (D). The profile of the ultrastructure of the Golgi apparatus (green) corresponds to the fluorescence image of TGN38 in (D). Blue: nucleus. (C) Scale bar: 50 μm; (E) scale bar: 1 μm.
From: Koga D, Kusumi S, Shibata M, Watanabe T.
Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure.
Front Neuroanat. 2021 Dec 2;15:759804.
doi: 10.3389/fnana.2021.759804.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
IF WB ResetSheep anti Rat TGN38
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- TGN38
Quick Links:
Antibody Quality and 
Unrivaled 
| Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993). TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
25% Glycerol - Immunogen
- Recombinant fusion protein corresponding to extracellular domain of TGN38.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
||
| Immunohistology - Frozen | |
0.1ug/ml | 1ug/ml |
| Western Blotting | |
0.1ug/ml | 1ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Immunohistology
- Fixation with methanol or methanol/acetone recommended.
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References for TGN38 antibody
-
Vo, Y.P. et al. (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells.
Biochem Biophys Res Commun. 324: 1004-10. -
Prabhu, Y. et al. (2014) Defective Transport of the Obesity Mutant PC1/3 N222D Contributes to Loss of Function.
Endocrinology. 155: 2391-401. -
Ni-Komatsu, L. et al. (2008) Identification of quinolines that inhibit melanogenesis by altering tyrosinase family trafficking.
Mol Pharmacol. 74:1576-86. -
Mathews, P.M. et al. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.
J Biol Chem. 277: 5299-307. -
Phillips, S.E. et al. (2006) Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.
Mol Biol Cell. 17: 2498-512. -
Waugh, M.G. et al. (2011) Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
J Lipid Res. 52: 582-9. -
Farah,. C.A. et al. (2006) Tau interacts with Golgi membranes and mediates their association with microtubules.
Cell Motil Cytoskeleton. 63: 710-24. -
Hesse, D. et al. (2010) Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1.
Biochem Biophys Res Commun. 394: 896-903.
View The Latest Product References
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Miranda-Saksena, M. et al. (2002) In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body.
J Virol. 76: 9934-51. -
Procino, G. et al. (2006) Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment.
Am J Physiol Renal Physiol. 290: F985-94. -
Han, L. et al. (2008) A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.
Mol Endocrinol. 22: 1935-49. -
Probst, O.C. et al. (2006) The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.
J Cell Sci. 119 (Pt 23): 4935-43. -
Wasmeier, C. et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.
J Cell Biol. 175: 271-81. -
Hou, J.C. et al. (2006) A specific dileucine motif is required for the GGA-dependent entry of newly synthesized insulin-responsive aminopeptidase into the insulin-responsive compartment.
J Biol Chem. 281: 33457-66. -
Fortin, M.E. et al. (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF.
Biol Reprod. 74: 2-12. -
Vasile, E. et al. (2006) IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.
Exp Cell Res. 312: 3132-41. -
Acquatella-Tran Van Ba, I. et al. (2011) Reg-1α is a new neuronal secreted factor that stimulates neurite outgrowth via the Exostosin tumor-like 3 (EXTL3) receptor.
J Biol Chem. 287: 4726-39. -
Watanabe, T. et al. (2012) A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.
J Histochem Cytochem. 60: 588-602. -
Yamamoto, H. et al. (2016) Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition.
Bone. 84: 120-30. -
Koga, D. et al. (2015) Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components.
J Histochem Cytochem. 63 (12): 968-79. -
Mukai, K. et al. (2016) Activation of STING requires palmitoylation at the Golgi.
Nat Commun. 7: 11932. -
Koga, D. et al. (2017) Changes in the three-dimensional ultrastructure of membranous organelles in male rat pituitary gonadotropes after castration.
Biomed Res. 38 (1): 1-18. -
Kusumi, S. et al. (2018) Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy.
Biomed Res. 39 (1): 21-25. -
Mukai, K. et al. (2021) Homeostatic regulation of STING by retrograde membrane traffic to the ER.
Nat Commun. 12 (1): 61. -
Caswell, P.T. & Dickens, M. (2018) JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Mol Cell Biochem. 444 (1-2): 1-13. -
Ogawa, E. et al. (2018) The binding of TBK1 to STING requires exocytic membrane traffic from the ER.
Biochem Biophys Res Commun. 503 (1): 138-145. -
Sou, Y.S. et al. (2019) Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice.
eNeuro. 6(3):ENEURO.0427-18.2019. -
Koga, D. et al. (2021) Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure.
Front Neuroanat. 15: 759804.
Further Reading
-
Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
Biochem J. 270: 97-102.
- RRID
- AB_2203272
- UniProt
- P19814
- Entrez Gene
- Tgoln1
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005768 endosome
- GO:0005794 Golgi apparatus
- GO:0006895 Golgi to endosome transport
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