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| Sheep anti Rat TGN38 antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).
TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to rat TGN38 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Recombinant fusion protein corresponding to extracellular domain of TGN38.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||0.1ug/ml||1ug/ml|
- Fixation with methanol or methanol/acetone recommended.
|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Rabbit anti Sheep IgG (H/L):Biotin||5184-2304||C E F WB||1.5 ml||Log in|
|List Price||Your Price|
|Description||Rabbit anti Sheep IgG (H/L):Biotin|
|Donkey anti Sheep/Goat IgG:DyLight®488||STAR88D488GA||F IF||0.1 mg||Log in|
|List Price||Your Price|
|Description||Donkey anti Sheep/Goat IgG:DyLight®488|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||C E P WB||1 ml||Log in|
|List Price||Your Price|
|Description||Donkey anti Sheep/Goat IgG:HRP|
References for TGN38 antibody
Vo, Y.P. et al. (2004) Recycling of the dense-core vesicle membrane protein phogrin in Min6 beta-cells.
Biochem Biophys Res Commun. 324: 1004-10.
Prabhu, Y. et al. (2014) Defective Transport of the Obesity Mutant PC1/3 N222D Contributes to Loss of Function.
Endocrinology. 155: 2391-401.
Ni-Komatsu, L. et al. (2008) Identification of quinolines that inhibit melanogenesis by altering tyrosinase family trafficking.
Mol Pharmacol. 74:1576-86.
Mathews, P.M. et al. (2002) Alzheimer's disease-related overexpression of the cation-dependent mannose 6-phosphate receptor increases Abeta secretion: role for altered lysosomal hydrolase distribution in beta-amyloidogenesis.
J Biol Chem. 277: 5299-307.
Phillips, S.E. et al. (2006) Specific and nonspecific membrane-binding determinants cooperate in targeting phosphatidylinositol transfer protein beta-isoform to the mammalian trans-Golgi network.
Mol Biol Cell. 17: 2498-512.
Waugh, M.G. et al. (2011) Detergent-free isolation and characterization of cholesterol-rich membrane domains from trans-Golgi network vesicles.
J Lipid Res. 52: 582-9.
Farah,. C.A. et al. (2006) Tau interacts with Golgi membranes and mediates their association with microtubules.
Cell Motil Cytoskeleton. 63: 710-24.
Hesse, D. et al. (2010) Altered GLUT4 trafficking in adipocytes in the absence of the GTPase Arfrp1.
Biochem Biophys Res Commun. 394: 896-903.
View The Latest Product References
Miranda-Saksena, M. et al. (2002) In rat dorsal root ganglion neurons, herpes simplex virus type 1 tegument forms in the cytoplasm of the cell body.
J Virol. 76: 9934-51.
Procino, G. et al. (2006) Adipocytes support cAMP-dependent translocation of aquaporin-2 from intracellular sites distinct from the insulin-responsive GLUT4 storage compartment.
Am J Physiol Renal Physiol. 290: F985-94.
Han, L. et al. (2008) A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.
Mol Endocrinol. 22: 1935-49.
Probst, O.C. et al. (2006) The 46-kDa mannose 6-phosphate receptor does not depend on endosomal acidification for delivery of hydrolases to lysosomes.
J Cell Sci. 119 (Pt 23): 4935-43.
Wasmeier, C. et al. (2006) Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes.
J Cell Biol. 175: 271-81.
Hou, J.C. et al. (2006) A specific dileucine motif is required for the GGA-dependent entry of newly synthesized insulin-responsive aminopeptidase into the insulin-responsive compartment.
J Biol Chem. 281: 33457-66.
Fortin, M.E. et al. (2006) Modulation of GJA1 turnover and intercellular communication by proinflammatory cytokines in the anterior pituitary folliculostellate cell line TtT/GF.
Biol Reprod. 74: 2-12.
Vasile, E. et al. (2006) IntraGolgi distribution of the Conserved Oligomeric Golgi (COG) complex.
Exp Cell Res. 312: 3132-41.
Acquatella-Tran Van Ba, I. et al. (2011) Reg-1α is a new neuronal secreted factor that stimulates neurite outgrowth via the Exostosin tumor-like 3 (EXTL3) receptor.
J Biol Chem. 287: 4726-39.
Watanabe, T. et al. (2012) A unique ball-shaped Golgi apparatus in the rat pituitary gonadotrope: its functional implications in relation to the arrangement of the microtubule network.
J Histochem Cytochem. 60: 588-602.
Yamamoto, H. et al. (2016) Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition.
Bone. 84: 120-30.
Koga, D. et al. (2015) Correlative Light and Scanning Electron Microscopy for Observing the Three-Dimensional Ultrastructure of Membranous Cell Organelles in Relation to Their Molecular Components.
J Histochem Cytochem. 63 (12): 968-79.
Mukai, K. et al. (2016) Activation of STING requires palmitoylation at the Golgi.
Nat Commun. 7: 11932.
Koga, D. et al. (2017) Changes in the three-dimensional ultrastructure of membranous organelles in male rat pituitary gonadotropes after castration.
Biomed Res. 38 (1): 1-18.
Kusumi, S. et al. (2018) Combination of a cryosectioning method and section scanning electron microscopy for immuno-scanning electron microscopy.
Biomed Res. 39 (1): 21-25.
Mukai, K. et al. (2021) Homeostatic regulation of STING by retrograde membrane traffic to the ER.
Nat Commun. 12 (1): 61.
Caswell, P.T. & Dickens, M. (2018) JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Mol Cell Biochem. 444 (1-2): 1-13.
Ogawa, E. et al. (2018) The binding of TBK1 to STING requires exocytic membrane traffic from the ER.
Biochem Biophys Res Commun. 503 (1): 138-145.
Sou, Y.S. et al. (2019) Cerebellar Neurodegeneration and Neuronal Circuit Remodeling in Golgi pH Regulator-Deficient Mice.
Koga, D. et al. (2021) Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure.
Front Neuroanat. 15: 759804.
Luzio, J.P. et al. (1990) Identification, sequencing and expression of an integral membrane protein of the trans-Golgi network (TGN38).
Biochem J. 270: 97-102.
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