IgM antibody
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Goat anti Rat IgM:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgM
Antibody Quality and Performance Guarantee
| Goat anti Rat IgM antibody recognizes rat IgM as demonstrated by ELISA. This antibody has been cross-absorbed against rat IgG, but may react with IgM from other species. |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Antiserum Preparation
- Antiserum to rat IgM was raised by repeated immunisation of goats with highly purified antigen. The preparation was purified by affinity chromatography on rat IgM covalently linked to agarose.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Normal rat IgM.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product is photosensitive and should be protected from light.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgM antibody
-
Brissette, C.A. et al. (2012) Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis.
J Neuroinflammation. 9: 249.
- RRID
- AB_616872
View more products with IGM specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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