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IgM antibody

Goat anti Rat IgM:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

Product Code Applications Pack Size List Price Your Price Qty
302009
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg
List Price Your Price

Goat anti Rat IgM antibody recognizes rat IgM as demonstrated by ELISA. This antibody has been cross-absorbed against rat IgG, but may react with IgM from other species.

Target Species
Rat
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Antiserum Preparation
Antiserum to rat IgM was raised by repeated immunisation of goats with highly purified antigen. The preparation was purified by affinity chromatography on rat IgM covalently linked to agarose.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose)
Immunogen
Normal rat IgM.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgM antibody

  1. Brissette, C.A. et al. (2012) Acetate supplementation reduces microglia activation and brain interleukin-1β levels in a rat model of Lyme neuroborreliosis.
    J Neuroinflammation. 9: 249.

ELISA

RRID
AB_616872
View more products with IGM specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
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