IgG antibody
Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody recognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.
We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
We recommend diluting Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
Product Details
- Target Species
- Rat
- Product Form
- F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Immunogen
- Whole rat IgG.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20759
- P20760
- P20761
- P20762
- Entrez Gene
- Ighg
- LOC679045
- LOC362795
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/5 | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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Product Specific References
References for IgG antibody
-
Bellingan. G. et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.
J. Exp. Med. 196:1515-1521. -
Broderick, C.A. et al. (2005) Local Administration of an Adeno-associated Viral Vector Expressing IL-10 Reduces Monocyte Infiltration and Subsequent Photoreceptor Damage during Experimental Autoimmune Uveitis
Mol Ther. 12: 369-73. -
Ko, Y.C. et al. (2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell–endothelium interactions
J Anat. 214: 183-95. -
Curran, C.S. et al. (2006) Lactoferrin activates macrophages via TLR4-dependent and -independent signaling pathways
Cell Immunol. 242: 23-30. -
Guilloteau, L.A. et al. (2003) Nramp1 Is Not a Major Determinant in the Control of Brucella melitensis Infection in Mice
Infect Immun. 71: 621-8. -
Maemura, K. et al. (2006) Antigen-presenting cells expressing glutamate decarboxylase 67 were identified as epithelial cells in glutamate decarboxylase 67-GFP knock-in mouse thymus.
Tissue Antigens. 67: 198-206.
Fluorescent Spectraviewer
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