IgG antibody
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Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Antibody Quality and Performance Guarantee
| FITC conjugated Goat (F(ab′)2 anti Rat IgG antibody recognises rat IgG. Cross-reactivity with mouse IgG has been reduced by adsorption, however, we recommend dilution in buffer containing 10% (v/v) normal mouse serum for use upon mouse tissues. |
- Target Species
- Rat
- Product Form
- F(ab')2 fragment of IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.5% Bovine Serum Albumin - Immunogen
- Rat IgG.
- Approx. Protein Concentrations
- F(ab')2 concentration 0.7 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Parish, N.M. et al. (1999) Anti-CD44 treatment does not prevent the extravasation of autopathogenic T cells to the thyroid in experimental autoimmune thyroiditis.
Immunology. 97: 533-9. -
Haraoka, M. et al. (1999) Neutrophil recruitment and resistance to urinary tract infection.
J Infect Dis. 180: 1220-9. -
Hang, L. et al. (2000) Interleukin-8 receptor knockout mice have subepithelial neutrophil entrapment and renal scarring following acute pyelonephritis.
J Infect Dis. 182: 1738-48. -
Fowles, L. et al. (2000) Regulation of urokinase plasminogen activator gene transcription in the RAW264 murine macrophage cell line by macrophage colony-stimulating factor (CSF-1) is dependent upon the level of cell surface receptor.
Biochem. J. 347: 313 - 320. -
Franke, H. et al. (2005) P2X(7) receptor-mRNA and -protein in the mouse retina; changes during retinal degeneration in BALBCrds mice.
Neurochem Int. 47: 235-42. -
Chapoval, S.P. et al. (2009) Lung vascular endothelial growth factor expression induces local myeloid dendritic cell activation.
Clin Immunol. 132: 371-84. -
Song, L. et al. (2011) Deletion of the murine scavenger receptor CD68.
J Lipid Res. 52: 1542-50. -
Modjtahedi, H. et al. (2012) Immunohistochemical discrimination of wild-type EGFR from EGFRvIII in fixed tumour specimens using anti-EGFR mAbs ICR9 and ICR10.
Br J Cancer. 106: 883-888
View The Latest Product References
-
Englezou, P.C. et al. (2015) P2X7R activation drives distinct IL-1 responses in dendritic cells compared to macrophages.
Cytokine. 74 (2): 293-304.
STAR69
149094
159653
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