IgG antibody
FITC conjugated Donkey anti Rat IgG antibody used to detect bound Rat anti Mouse CD31 antibody in a choroidal neovascularization study using immunofluorescence microscopy.
Image caption:
Choroidal neovascularization (CNV) detection by CD31 immunofluorescence. Mice that received only the vehicle (upper left, group V) had larger areas of CNV than mice that received the supplement (upper right) and/or anti-vascular endothelial growth factor (VEGF) treatment. Treatment groups included the nutritional supplement (S) group, the supplement and anti-vascular endothelial growth factor agent (S + aVEGF) group, and the vehicle and anti-VEGF agent (V + aVEGF) group.
From: Ivanescu AA, Fernández-Robredo P, Heras-Mulero H, Sádaba-Echarri LM, García-García L, Fernández-García V, Moreno-Orduna M, Redondo-Exposito A, Recalde S, García-Layana A.
Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 2015 Jul 6;7(7):5423-42.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Donkey anti Rat IgG (H/L):FITC (Mouse Adsorbed)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
| Donkey anti Rat IgG antibody recognizes rat IgG heavy and light chains and demonstrates minimal cross reactivity with mouse serum proteins. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Rat IgG.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Bombardieri, M. et al. (2011) A BAFF/APRIL-dependent TLR3-stimulated pathway enhances the capacity of rheumatoid synovial fibroblasts to induce AID expression and Ig class-switching in B cells.
Ann Rheum Dis. 70 (10): 1857-65. -
Ivanescu, A.A. et al. (2015) Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 7 (7): 5423-42.
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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