IgG antibody
FITC conjugated Donkey anti Rat IgG antibody used to detect bound Rat anti Mouse CD31 antibody in a choroidal neovascularization study using immunofluorescence microscopy.
Image caption:
Choroidal neovascularization (CNV) detection by CD31 immunofluorescence. Mice that received only the vehicle (upper left, group V) had larger areas of CNV than mice that received the supplement (upper right) and/or anti-vascular endothelial growth factor (VEGF) treatment. Treatment groups included the nutritional supplement (S) group, the supplement and anti-vascular endothelial growth factor agent (S + aVEGF) group, and the vehicle and anti-VEGF agent (V + aVEGF) group.
From: Ivanescu AA, Fernández-Robredo P, Heras-Mulero H, Sádaba-Echarri LM, García-García L, Fernández-García V, Moreno-Orduna M, Redondo-Exposito A, Recalde S, García-Layana A.
Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 2015 Jul 6;7(7):5423-42.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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IF ResetDonkey anti Rat IgG (H/L) (Mouse Adsorbed)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
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Antibody Quality and 
Unrivaled 
| Donkey anti Rat IgG antibody recognizes rat IgG heavy and light chains and demonstrates minimal cross reactivity with mouse serum proteins. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig Fraction - liquid
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Rat IgG.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
-20ºC only (ship +4ºC)
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IgG antibody
-
Bombardieri, M. et al. (2011) A BAFF/APRIL-dependent TLR3-stimulated pathway enhances the capacity of rheumatoid synovial fibroblasts to induce AID expression and Ig class-switching in B cells.
Ann Rheum Dis. 70 (10): 1857-65. -
Ivanescu, A.A. et al. (2015) Modifying Choroidal Neovascularization Development with a Nutritional Supplement in Mice.
Nutrients. 7 (7): 5423-42.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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