IgG antibody
Horseradish peroxidase conjugated Goat anti Rat IgG antibody (5204-2504) used for the detection of bound Rat anti tubulin-α antibody (MCA78G) by western blotting
Image caption:
Protein expression and solubility. Analysis of the expression and the soluble/insoluble partition of Aβwt-GFP and Aβm-GFP. The total, soluble and insoluble fractions were separated after induction. Tubulin alpha (Tubα) was employed to normalise the Aβ-GFP bands.
From: Sanchez de Groot N, Gomes RA, Villar-Pique A, Babu MM, Coelho AV, Ventura S. Proteome response at the edge of protein aggregation. Open Biol. 2015 Feb;5(2). pii: 140221.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Horseradish peroxidase conjugated Goat anti Rat IgG antibody recognizes Rat IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgG and rat serum.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Gentamicin Sulphate 1% Bovine Serum Albumin - Immunogen
- Rat IgG, whole molecule.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01836
- P20766
- P20760
- P20759
- P20761
- P01835
- P20767
- P20762
- Entrez Gene
- LOC500180
- Ighg
- LOC679045
- LOC362795
- RGD1564318
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50000 | 1/100000 | |
| Immunohistology - Frozen | 1/500 | 1/1500 | |
| Western Blotting | 1/2000 | 1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml |
Product Specific References
References for IgG antibody
-
Sanchez de Groot, N. et al. (2015) Proteome response at the edge of protein aggregation.
Open Biol. 5. pii: 140221.
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