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IgG antibody

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Anti Rat IgG (H/L) Antibody thumbnail image 1 Anti Rat IgG (H/L) Antibody thumbnail image 2 Anti Rat IgG (H/L) Antibody thumbnail image 3
Anti Rat IgG (H/L) Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Rat IgG antibody (5204-2504) used for the detection of bound Rat anti tubulin-α antibody (MCA78G) by western blotting
Image caption:
Protein expression and solubility. Analysis of the expression and the soluble/insoluble partition of Aβwt-GFP and Aβm-GFP. The total, soluble and insoluble fractions were separated after induction. Tubulin alpha (Tubα) was employed to normalise the Aβ-GFP bands.

From: Sanchez de Groot N, Gomes RA, Villar-Pique A, Babu MM, Coelho AV, Ventura S. Proteome response at the edge of protein aggregation. Open Biol. 2015 Feb;5(2). pii: 140221.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat IgG (H/L) Antibody gallery image 2
Published customer image:
Horseradish peroxidase conjugated Goat anti Rat IgG antibody (5204-2504) used to detect bound Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957) for immunohistochemistry on formalin fixed cryosections.
Image caption:
Mean density of CD68+ macrophages (count/muscle fiber count) in (a) gastrocnemius muscle with (b) representative immunolocalizations of CD68+ macrophages (see arrows) and in (c) soleus muscle with (d) representative immunolocalizations of CD68+ macrophages (see arrows) within the four groups WT, PanIN 1A-B, PanIN 2-3, and PDAC as indicated. Mean ± SEM, ** for p >0.01 and *** for p >0.001 vs. WT, # for p <0.05 vs. PanIN 2-3, and §§ for p <0.01 vs. PanIN 1A-B by Student’s t-test or Mann–Whitney U test (posthoc) for unpaired observation.

From: Hildebrandt W, Keck J, Schmich S, Bonaterra GA, Wilhelm B, Schwarzbach H, Eva A, Bertoune M, Slater EP, Fendrich V, Kinscherf R.
Inflammation and Wasting of Skeletal Muscles in Kras-p53-Mutant Mice with Intraepithelial Neoplasia and Pancreatic Cancer-When Does Cachexia Start?
Cells. 2022 May 11;11(10):1607.
doi: 10.3390/cells11101607.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat IgG (H/L) Antibody gallery image 3
Published customer image:
Horseradish peroxidase conjugated Goat anti Rat IgG antibody (5204-2504) used to detect bound Rat anti Mouse COX2 antibody by immunohistochemistry on formalin fixed cryosections.
Image caption:
Mean density of COX2+ cells (count/muscle fiber count) in (a) gastrocnemius muscle with (b) representative immunolocalizations of COX2+ cells (see arrows) and in (c) soleus muscle with (d) representative immunolocalizations of COX2+ cells (see arrows) within the four groups WT, PanIN 1A-B, PanIN 2-3, and PDAC as indicated. Mean ± SEM, *** for p> 0.001 vs. WT, # for p <0.05 vs. PanIN 2-3 by Student’s t-test or Mann–Whitney U test (posthoc) for unpaired observation.

From: Hildebrandt W, Keck J, Schmich S, Bonaterra GA, Wilhelm B, Schwarzbach H, Eva A, Bertoune M, Slater EP, Fendrich V, Kinscherf R.
Inflammation and Wasting of Skeletal Muscles in Kras-p53-Mutant Mice with Intraepithelial Neoplasia and Pancreatic Cancer-When Does Cachexia Start?
Cells. 2022 May 11;11(10):1607.
doi: 10.3390/cells11101607.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.rel="noopener">Creative Commons Attribution License.

Goat anti Rat IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Product Code Applications Pack Size List Price Your Price Qty
5204-2504
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C E WB 2 mg loader
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Horseradish peroxidase conjugated Goat anti Rat IgG antibody recognizes Rat IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgG and rat serum.

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin Sulphate
1%Bovine Serum Albumin
Immunogen
Rat IgG, whole molecule.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000 1/100000
Immunohistology - Frozen 1/500 1/1500
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Hildebrandt, W. et al. (2022) Inflammation and Wasting of Skeletal Muscles in Kras-p53-Mutant Mice with Intraepithelial Neoplasia and Pancreatic Cancer-When Does Cachexia Start?
    Cells. 11 (10): 1607.
  2. Sanchez de Groot, N. et al. (2015) Proteome response at the edge of protein aggregation.
    Open Biol. 5. pii: 140221.

5204-2504

147110 154073 158319 163418

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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