IgG antibody

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Goat anti Rat IgG (H/L):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
5204-2504 C E WB 2 mg
Horseradish peroxidase conjugated Goat anti Rat IgG antibody recognizes Rat IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgG and rat serum.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Gentamicin Sulphate
1%Bovine Serum Albumin
Immunogen
Rat IgG, whole molecule.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50000 1/100000
Immunohistology - Frozen 1/500 1/1500
Western Blotting 1/2000 1/10000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Sanchez de Groot, N. et al. (2015) Proteome response at the edge of protein aggregation.
    Open Biol. 5. pii: 140221.