Endothelin B Receptor antibody

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Sheep anti Rat Endothelin B Receptor (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AAR43
Datasheet
IC IF IP P
MSDS
0.1 mg loader

Sheep anti Rat endothelin B receptor antibody recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. Sheep anti Rat endothelin B receptor antibody (AAR43) recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. AAR43 does not block the binding of endothelins.

Sheep anti Rat endothelin B receptor antibody (AAR43) is suitable for use in immunocytochemistry on acetone fixed cells.

Product Details

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to rat ET-B (CT) was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.
Approx. Protein Concentrations
IgG concentration 1.41mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P21451 Related reagents
P24530 Related reagents
P48302 Related reagents
Entrez Gene
Ednrb Related reagents
EDNRB Related reagents
Ednrb Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001600 endothelin-B receptor activity
GO:0001934 positive regulation of protein phosphorylation
GO:0016021 integral to membrane
GO:0005624 membrane fraction
GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0008217 regulation of blood pressure
GO:0030318 melanocyte differentiation
GO:0031702 type 1 angiotensin receptor binding
GO:0031965 nuclear membrane
GO:0042310 vasoconstriction
GO:0042311 vasodilation
GO:0043066 negative regulation of apoptosis
GO:0048265 response to pain
GO:0048484 enteric nervous system development
GO:0050678 regulation of epithelial cell proliferation
GO:0051930 regulation of sensory perception of pain
GO:0005515 protein binding
GO:0001755 neural crest cell migration
GO:0006885 regulation of pH
GO:0007422 peripheral nervous system development
GO:0007497 posterior midgut development
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0014043 negative regulation of neuron maturation
Regulatory
For research purposes only

Applications of Endothelin B Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry
Immunofluorescence 10 20ug/ml
Immunohistology - Paraffin 10 20ug/ml
Immunoprecipitation 20 40ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml