Endothelin B Receptor antibody
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Sheep anti Rat endothelin B receptor antibody recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. Sheep anti Rat endothelin B receptor antibody (AAR43) recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. AAR43 does not block the binding of endothelins.
Sheep anti Rat endothelin B receptor antibody (AAR43) is suitable for use in immunocytochemistry on acetone fixed cells.
Sheep anti Rat endothelin B receptor antibody (AAR43) is suitable for use in immunocytochemistry on acetone fixed cells.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to rat ET-B (CT) was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.
- Approx. Protein Concentrations
- IgG concentration 1.41mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21451
- P24530
- P48302
- Entrez Gene
- Ednrb
- EDNRB
- Ednrb
- GO Terms
- GO:0005886 plasma membrane
- GO:0001600 endothelin-B receptor activity
- GO:0001934 positive regulation of protein phosphorylation
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
- GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0008217 regulation of blood pressure
- GO:0030318 melanocyte differentiation
- GO:0031702 type 1 angiotensin receptor binding
- GO:0031965 nuclear membrane
- GO:0042310 vasoconstriction
- GO:0042311 vasodilation
- GO:0043066 negative regulation of apoptosis
- GO:0048265 response to pain
- GO:0048484 enteric nervous system development
- GO:0050678 regulation of epithelial cell proliferation
- GO:0051930 regulation of sensory perception of pain
- GO:0005515 protein binding
- GO:0001755 neural crest cell migration
- GO:0006885 regulation of pH
- GO:0007422 peripheral nervous system development
- GO:0007497 posterior midgut development
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0014043 negative regulation of neuron maturation
- Regulatory
- For research purposes only
Applications of Endothelin B Receptor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry | |||
| Immunofluorescence | 10 | 20ug/ml | |
| Immunohistology - Paraffin | 10 | 20ug/ml | |
| Immunoprecipitation | 20 | 40ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml |
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