Endothelin B Receptor antibody

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Sheep anti Rat Endothelin B Receptor (C-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
AAR43 Datasheet	IC IF IP P SDS	0.1 mg	Log in		Get Quote

Sheep anti Rat endothelin B receptor antibody recognizes an epitope within the C-terminal (CT) region of endothelin B receptor (ET-B), a widely distributed transmembrane protein and member of the G-protein coupled receptor 1 family, which acts as a non-specific receptor for endothelin 1, 2, and 3, and mediates its action by G protein association and activation of a phosphatidylinositol-calcium second messenger system. Sheep anti Rat endothelin B receptor antibody (AAR43) recognizes epitopes on the cytoplasmic side of the receptor and therefore the cell membrane must be permeable to the antibody. AAR43 does not block the binding of endothelins.

Sheep anti Rat endothelin B receptor antibody (AAR43) is suitable for use in immunocytochemistry on acetone fixed cells.

Product Details

Target Species

Rat

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Human

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antiserum to rat ET-B (CT) was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

<0.1% Sodium Azide (NaN₃)

Immunogen

Keyhole limpet haemocyanin (KLH) conjugated C-terminal peptide KANDHGYDNFRSSNN corresponding to amino acids 424 to 437 of rat endothelin B receptor.

Approx. Protein Concentrations

IgG concentration 1.41mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P21451; P24530; P48302
Entrez Gene: Ednrb; EDNRB; Ednrb
GO Terms: GO:0005886 plasma membrane; GO:0001600 endothelin-B receptor activity; GO:0001934 positive regulation of protein phosphorylation; GO:0016021 integral to membrane; GO:0005624 membrane fraction; GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0008217 regulation of blood pressure; GO:0030318 melanocyte differentiation; GO:0031702 type 1 angiotensin receptor binding; GO:0031965 nuclear membrane; GO:0042310 vasoconstriction; GO:0042311 vasodilation; GO:0043066 negative regulation of apoptosis; GO:0048265 response to pain; GO:0048484 enteric nervous system development; GO:0050678 regulation of epithelial cell proliferation; GO:0051930 regulation of sensory perception of pain; GO:0005515 protein binding; GO:0001755 neural crest cell migration; GO:0006885 regulation of pH; GO:0007422 peripheral nervous system development; GO:0007497 posterior midgut development; GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter; GO:0014043 negative regulation of neuron maturation
Regulatory: For research purposes only

Applications of Endothelin B Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunocytochemistry
Immunofluorescence	10	20ug/ml
Immunohistology - Paraffin	10	20ug/ml
Immunoprecipitation	20	40ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	C E F WB	1.5 ml	Log in	Get Quote
Donkey anti Sheep/Goat IgG:DyLight®488	STAR88D488GA	F IF	0.1 mg	Log in	Get Quote
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml	Log in		Get Quote
100x Antigen Retrieval Buffer, pH8.0	BUF025C	P	50 ml	Log in		Get Quote

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