IgG antibody
Goat anti Rabbit IgG (H/L):FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
- Region
- (H/L)
Antibody Quality and Performance Guarantee
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Goat anti Rabbit IgG (H/L) antibody is a fluorescein-conjugated polyclonal antibody that recognizes both the heavy and light chains of rabbit Immunoglobulin G. This antibody is reported to be suitable for use in Flow cytometry and Immunofluorescence applications. Immunoglobulin G is a major component of humoral immunity and constitutes approximately 75% of serum immunoglobulins. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate (FITC) - lyophilized
- Reconstitution
- Use 1 ml sterile distilled water. For extended storage use 50% glycerol.
- Preparation
- IgG purified by Immunoaffinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Immunogen
- Rabbit IgG whole molecule.
- Purity
- Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rabbit IgG and rabbit serum.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is photosensitive and should be stored protected from light.
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product is photosensitive and should be stored protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/2000 | 1/10000 |
| Immunofluorescence | |
1/500 | 1/2500 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Suphatpahirapol, C. et al. (2019) Expression of a leptospiral leucine-rich repeat protein using a food-grade vector in Lactobacillus plantarum, as a strategy for vaccine delivery.
3 Biotech. 9 (9): 324. -
Scales, S.J. et al. (2020) Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.
J Am Soc Nephrol. 31 (9): 2044-64.
- RRID
- AB_616787
- UniProt
- P01870
- P01841
- P01838
- P01847
- P01840
- P01839
- Entrez Gene
- IGKC2
- LOC100009097
- GO Terms
- GO:0003823 antigen binding
5196-2404
149276
153710
158794
165584
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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