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IgG antibody

Goat anti Rabbit IgG (H/L):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
5196-2404
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Goat anti Rabbit IgG (H/L) antibody recognizes rabbit Immunoglobulin G heavy and light chains.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate (FITC) - lyophilized
Reconstitution
Use 1 ml sterile distilled water. For extended storage use 50% glycerol.
Preparation
IgG purified by Immunoaffinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
Rabbit IgG whole molecule.
Purity
Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rabbit IgG and rabbit serum.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2000 1/10000
Immunofluorescence 1/500 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Suphatpahirapol, C. et al. (2019) Expression of a leptospiral leucine-rich repeat protein using a food-grade vector in Lactobacillus plantarum, as a strategy for vaccine delivery.
    3 Biotech. 9 (9): 324.

Flow Cytometry

RRID
AB_616787
UniProt
P01870
P01841
P01838
P01847
P01840
P01839
Entrez Gene
IGKC2
LOC100009097
GO Terms
GO:0003823 antigen binding

5196-2404

149276 153710 158794 165584

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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