IgG antibody
StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies as described in the table above.
* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP)
Composite blot image with four distinct proteins visualized using StarBright Blue 520 and StarBright Blue 700 Secondary Antibodies, in combination with DyLight 800, and a rhodamine labeled recombinant human hFAB antibody for the detection of popular housekeeping proteins on the ChemiDoc MP Imaging System.
Goat ANTI-RABBIT IgG StarBright™ Blue 520
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Quick Links:
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| The StarBright Blue 520 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 488 nm and emission maximum at 520 nm. The StarBright Fluorophore is made up of multiple light-absorbing and -emitting monomers which provide an exceptionally bright signal compared to most traditional fluorophores. StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot. Features and Benefits Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 488 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes Low nonspecific binding — conjugated to highly cross-adsorbed antibodies Goat Anti-Rabbit IgG StarBright Blue 520 has been cross-adsorbed against bovine, goat, human, mouse and rat. |
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to StarBright Blue 520 - lyophilized
- Reconstitution
- Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
- Antiserum Preparation
- Antiserum to Rabbit IgG was raised by repeated immunisation of Goats with highly purified antigen.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- >80% Sucrose
<1% Polyethylene Glycol
<0.04% 2-Methyl-2H-Isothiazol-3-One
<10% Bovine Serum Albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Blue 520 488 520 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product is photosensitive and should be protected from light
DO NOT FREEZE the solubilized material
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | |||
| Western Blotting |  |
1/2,500 | 1/5,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- StarBright Blue 520 Fluorescent Secondary Antibodies can be used for multiplex fluorescent western blotting with Bio-Rad's ultra-sensitive near IR StarBright Blue 700 Antibodies and the hFAB Rhodamine Anti-Housekeeping Antibodies.
Note: StarBright Antibodies are not suitable for stripping and reprobing. - Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12005866.pdf
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12005866.pdf
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgG antibody
-
Abbott, C.N. et al. (2021) Expression of Genes Located on the Incompatibility Group FIB Plasmids at Transcription and Protein Levels in Iron-Modified Growth Conditions.
Front Microbiol. 12: 729275. -
Baumann, C. et al. (2021) Changes in chromatin accessibility landscape and histone H3 core acetylation during valproic acid-induced differentiation of embryonic stem cells.
Epigenetics Chromatin. 14 (1): 58. -
Riera-Tur, I. et al. (2022) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicity.
Life Sci Alliance.5(3):e202101185. -
Cejas, R.B. et al. (2022) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived Cardiomyocytes.
Cardiovasc Toxicol. May 21 [Epub ahead of print].
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