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RIP3 antibody

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Anti RIP3 Antibody thumbnail image 1 Anti RIP3 Antibody thumbnail image 2
Anti RIP3 Antibody gallery image 1
Western blot analysis of whole cell lysate from mouse 3T3 cells probed with Rabbit anti RIP3 (AHP1797) in the absence (A) or presence (B) of blocking peptide
Anti RIP3 Antibody gallery image 2
Immunohistochemical staining of rat kidney with Rabbit anti RIP3 (AHP1797).

Rabbit anti RIP3

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1797
Datasheet
P* WB
MSDS
0.1 mg loader

Rabbit anti RIP3 antibody recognizes mouse serine-threonine kinase receptor-interacting protein 3 (RIP3). RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its C-terminus. RIP3 activates NF-Kappa B and potently induces apoptosis.

RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to mouse RIP3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence corresponding to amino acids 473-486 of mouse RIP3.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q9QZL0
Entrez Gene
Ripk3
GO Terms
GO:0005515 protein binding
GO:0006915 apoptosis
GO:0004704 NF-kappaB-inducing kinase activity
GO:0005524 ATP binding
GO:0005737 cytoplasm
GO:0006468 protein phosphorylation
GO:0006917 induction of apoptosis
GO:0007249 I-kappaB kinase/NF-kappaB cascade
Regulatory
For research purposes only

Applications of RIP3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Rat kidney
Western Blotting
AHP1797 detects a band of approximately 56 kDa in Mouse 3T3 whole cell lysate
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
Histar Detection System STAR3000A C P 50 Tests

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for RIP3 antibody

  1. Günther, C. et al. (2011) Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.
    Nature. 477: 335-9.
  2. Meng, L. et al. (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
    Proc Natl Acad Sci U S A. 112 (35): 11007-12.
  3. Huang, S.C. et al. (2014) Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.
    Sci Signal. 7 (314): ra20.
  4. Mizumura, K. et al. (2014) Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.
    J Clin Invest. 124 (9): 3987-4003.
  5. Feng, S. et al. (2007) Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain.
    Cell Signal. 19 (10): 2056-67.
  6. Mizumura, K. et al. (2018) Sphingolipid regulation of lung epithelial cell mitophagy and necroptosis during cigarette smoke exposure.
    FASEB J. 32 (4): 1880-90.
  7. Najafov, A. et al. (2019) TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL.
    Mol Cell. 75 (3): 457-468.e4.

Further Reading

  1. Yu, P.W. et al. (1999) Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB.
    Curr Biol. 9 (10): 539-42.
  2. Sun, X., et al (1999) RIP3, a novel apoptosis-inducing kinase.
    J Biol Chem. 274:16871-5.
  3. Pazdernik, N.J., et al (1999) Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB.
    Mol Cell Bio.19:6500-8
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