RIP3 antibody
Rabbit anti RIP3 antibody recognizes mouse serine-threonine kinase receptor-interacting protein 3 (RIP3). RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its C-terminus. RIP3 activates NF-Kappa B and potently induces apoptosis.
RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.
RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to mouse RIP3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide sequence corresponding to amino acids 473-486 of mouse RIP3.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9QZL0
- Entrez Gene
- Ripk3
- GO Terms
- GO:0005515 protein binding
- GO:0006915 apoptosis
- GO:0004704 NF-kappaB-inducing kinase activity
- GO:0005524 ATP binding
- GO:0005737 cytoplasm
- GO:0006468 protein phosphorylation
- GO:0006917 induction of apoptosis
- GO:0007249 I-kappaB kinase/NF-kappaB cascade
- Regulatory
- For research purposes only
Applications of RIP3 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Rat kidney
- Western Blotting
- AHP1797 detects a band of approximately 56 kDa in Mouse 3T3 whole cell lysate
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for RIP3 antibody
-
Günther, C. et al. (2011) Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.
Nature. 477: 335-9. -
Meng, L. et al. (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
Proc Natl Acad Sci U S A. 112 (35): 11007-12. -
Huang, S.C. et al. (2014) Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.
Sci Signal. 7 (314): ra20. -
Mizumura, K. et al. (2014) Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.
J Clin Invest. 124 (9): 3987-4003. -
Feng, S. et al. (2007) Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain.
Cell Signal. 19 (10): 2056-67. -
Mizumura, K. et al. (2018) Sphingolipid regulation of lung epithelial cell mitophagy and necroptosis during cigarette smoke exposure.
FASEB J. 32 (4): 1880-90. -
Najafov, A. et al. (2019) TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL.
Mol Cell. 75 (3): 457-468.e4.
Further Reading
-
Yu, P.W. et al. (1999) Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB.
Curr Biol. 9 (10): 539-42. -
Sun, X., et al (1999) RIP3, a novel apoptosis-inducing kinase.
J Biol Chem. 274:16871-5. -
Pazdernik, N.J., et al (1999) Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB.
Mol Cell Bio.19:6500-8
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
