RIP3 antibody

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin 1			5ug/ml
Western Blotting		0.5	1.0ug/ml

1. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Histology Positive Control Tissue

Rat kidney

Western Blotting

AHP1797 detects a band of approximately 56 kDa in Mouse 3T3 whole cell lysate

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

References for RIP3 antibody

1. Günther, C. et al. (2011) Caspase-8 regulates TNF-α-induced epithelial necroptosis and terminal ileitis.
   Nature. 477: 335-9.
2. Meng, L. et al. (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality.
   Proc Natl Acad Sci U S A. 112 (35): 11007-12.
3. Huang, S.C. et al. (2014) Tumor necrosis factor suppresses NR5A2 activity and intestinal glucocorticoid synthesis to sustain chronic colitis.
   Sci Signal. 7 (314): ra20.
4. Mizumura, K. et al. (2014) Mitophagy-dependent necroptosis contributes to the pathogenesis of COPD.
   J Clin Invest. 124 (9): 3987-4003.
5. Feng, S. et al. (2007) Cleavage of RIP3 inactivates its caspase-independent apoptosis pathway by removal of kinase domain.
   Cell Signal. 19 (10): 2056-67.
6. Mizumura, K. et al. (2018) Sphingolipid regulation of lung epithelial cell mitophagy and necroptosis during cigarette smoke exposure.
   FASEB J. 32 (4): 1880-90.
7. Najafov, A. et al. (2019) TAM Kinases Promote Necroptosis by Regulating Oligomerization of MLKL.
   Mol Cell. 75 (3): 457-468.e4.

Further Reading

1. Yu, P.W. et al. (1999) Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB.
   Curr Biol. 9 (10): 539-42.
2. Sun, X., et al (1999) RIP3, a novel apoptosis-inducing kinase.
   J Biol Chem. 274:16871-5.
3. Pazdernik, N.J., et al (1999) Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB.
   Mol Cell Bio.19:6500-8

Immunofluorescence

Western Blotting

RRID

AB_2178676

UniProt

Q9QZL0

Entrez Gene

Ripk3

GO Terms

GO:0005515 protein binding

GO:0006915 apoptosis

GO:0004704 NF-kappaB-inducing kinase activity

GO:0005524 ATP binding

GO:0005737 cytoplasm

GO:0006468 protein phosphorylation

GO:0006917 induction of apoptosis

GO:0007249 I-kappaB kinase/NF-kappaB cascade