Methotrexate antibody

Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-
Aminopterin | 18% |
Folic acid | <0.01% |
Dyhydrofolic acid | <0.01% |
Tetrahydrofolic acid | <0.01% |
N-5-Methyltetrahydrofolic acid | <0.01% |
Folinic acid | <0.01% |
N-(p-Aminobenzoyl)-L-glutamic acid | <0.01% |
Product Details
- Target Species
- Chemical
- Product Form
- Serum - liquid
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Methotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Methotrexate antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Product Specific References
References for Methotrexate antibody
-
Suzuki, Y. et al. (2003) The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.
Anal Sci. 19 (1): 111-5. -
Tesfalidet, S. et al. (2016) Detection of methotrexate in a flow system using electrochemical impedance spectroscopy and multivariate data analysis.
Anal Chim Acta. 914: 1-6. -
Sereilakena, Pet al. (2017) Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
Analytica Chimica Acta. 987: 15-24. -
Phal, S. et al. (2018) Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
Journal of Electroanalytical Chemistry. Jan 3 [Epub ahead of print].
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