Methotrexate antibody

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Sheep anti Methotrexate

Product Type
Polyclonal Antibody
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0.5 ml

Sheep anti methotrexate antibody recognizes Methotrexate, a drug originally manufactured as a chemotherapeutic agent for the treatment or a number of cancers. Methotrexate also acts as a disease-modifying anti-rheumatic drug used for the treatment of autoimmune conditions such as rheumatoid arthritis, Chron's disease and vasculitis.

Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-

Folic acid<0.01%
Dyhydrofolic acid<0.01%
Tetrahydrofolic acid<0.01%
N-5-Methyltetrahydrofolic acid<0.01%
Folinic acid<0.01%
N-(p-Aminobenzoyl)-L-glutamic acid<0.01%

Product Details

Target Species
Product Form
Serum - liquid
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Methotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH.

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

For research purposes only

Applications of Methotrexate antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for Methotrexate antibody

  1. Suzuki, Y. et al. (2003) The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.
    Anal Sci. 19 (1): 111-5.
  2. Tesfalidet, S. et al. (2016) Detection of methotrexate in a flow system using electrochemical impedance spectroscopy and multivariate data analysis.
    Anal Chim Acta. 914: 1-6.
  3. Sereilakena, Pet al. (2017) Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
    Analytica Chimica Acta. 987: 15-24.
  4. Phal, S. et al. (2018) Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
    Journal of Electroanalytical Chemistry. Jan 3 [Epub ahead of print].
  5. Mwanza, D. et al. (2021) Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces: construction of capacitive biosensor for methotrexate detection
    Electrochimica Acta. : 139360. [Epub ahead of print]

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