Methotrexate antibody
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Sheep anti methotrexate antibody recognizes Methotrexate, a drug originally manufactured as a chemotherapeutic agent for the treatment or a number of cancers. Methotrexate also acts as a disease-modifying anti-rheumatic drug used for the treatment of autoimmune conditions such as rheumatoid arthritis, Chron's disease and vasculitis.
Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-
Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-
| Aminopterin | 18% |
| Folic acid | <0.01% |
| Dyhydrofolic acid | <0.01% |
| Tetrahydrofolic acid | <0.01% |
| N-5-Methyltetrahydrofolic acid | <0.01% |
| Folinic acid | <0.01% |
| N-(p-Aminobenzoyl)-L-glutamic acid | <0.01% |
Product Details
- Target Species
- Chemical
- Product Form
- Serum - liquid
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Methotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Methotrexate antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Methotrexate antibody
-
Suzuki, Y. et al. (2003) The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.
Anal Sci. 19 (1): 111-5. -
Tesfalidet, S. et al. (2016) Detection of methotrexate in a flow system using electrochemical impedance spectroscopy and multivariate data analysis.
Anal Chim Acta. 914: 1-6. -
Sereilakena, Pet al. (2017) Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
Analytica Chimica Acta. 987: 15-24. -
Phal, S. et al. (2018) Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
Journal of Electroanalytical Chemistry. Jan 3 [Epub ahead of print]. -
Mwanza, D. et al. (2021) Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces: construction of capacitive biosensor for methotrexate detection
Electrochimica Acta. : 139360. [Epub ahead of print]
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