Methotrexate antibody

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Sheep anti Methotrexate

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: Methotrexate

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Secondary Antibodies
References (5)
Applications
Storage Information

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6120-0004 Datasheet Datasheet SDS SDS	E	0.5 ml		Log in	Get Quote
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Sheep anti methotrexate antibody recognizes Methotrexate, a drug originally manufactured as a chemotherapeutic agent for the treatment or a number of cancers. Methotrexate also acts as a disease-modifying anti-rheumatic drug used for the treatment of autoimmune conditions such as rheumatoid arthritis, Chron's disease and vasculitis.

Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-

Aminopterin	18%
Folic acid	<0.01%
Dyhydrofolic acid	<0.01%
Tetrahydrofolic acid	<0.01%
N-5-Methyltetrahydrofolic acid	<0.01%
Folinic acid	<0.01%
N-(p-Aminobenzoyl)-L-glutamic acid	<0.01%

Target Species: Chemical
Product Form: Serum - liquid
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Immunogen: Methotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH.
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Methotrexate antibody

Suzuki, Y. et al. (2003) The immunoassay of methotrexate by capillary electrophoresis with laser-induced fluorescence detection.
Anal Sci. 19 (1): 111-5.
Tesfalidet, S. et al. (2016) Detection of methotrexate in a flow system using electrochemical impedance spectroscopy and multivariate data analysis.
Anal Chim Acta. 914: 1-6.
Sereilakena, Pet al. (2017) Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
Analytica Chimica Acta. 987: 15-24.
Phal, S. et al. (2018) Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
Journal of Electroanalytical Chemistry. Jan 3 [Epub ahead of print].
Mwanza, D. et al. (2021) Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces: construction of capacitive biosensor for methotrexate detection
Electrochimica Acta. : 139360. [Epub ahead of print]