von Willebrand Factor antibody
Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated aortic cell preparations by immunofluorescence.
Image caption:
Characterization of the isolated cells.
(A) Representative phase contrast micrographs show the cell population collected at each of the four consecutive rounds of digestion (well 1 to 4) on the intimal (I) and adventitial (A) side of the aorta. In both cases, three main phenotypes were observed: small polygonal cells forming areas of cobblestone appearance, giant mono- or multinucleated cells and elongated cells. All three phenotypes were also detected at P1. Immunofluorescence analysis performed on each P0 population identified ECs by positive vWf staining (insert). The small polygonal mononucleated cells and the giant mono- or polynucleated morphologies, stained both for vWf. Rare elongated cells could also be vWf positive, their unusual shape likely being due to surrounding peer pressure. Magnification of the phase contrast pictures and immunofluorescence images is 10x and 63x, respectively. (B) Immunofluorescent micrographs showing the diversity of vWf presentation. vWf staining performed on adventitial aortic endothelial cells shows diverse and typical vWF staining patterns such as that of elongated WPBs throughout the cell body (d and e), residual vWf granules after degranulation of WPB or seen in cross section (a), reticular vWf around the nucleus corresponding to vWf re-synthesis after degranulation (b and c) or extracellular string of vWf (e). (C) Immunofluorescent micrographs of isolated cells double-stained for vWf together with either CD31 or VE-cadherin validates vWf staining. Small polygonal mono-nucleated cells and most of the giant mono- or polynucleated vWf positive cells also stained for CD31 or VE-cadherin at P0 and P2 and in IEC and AEC.
From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. (2015)
A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS ONE 10(11): e0143144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated proliferating aortic cell preparations by immunofluorescence.
Image caption:
Analysis of proliferating cells: selection of EC-enriched fractions and validation of procedure.
(A) The left-hand morphologic diagram (M) shows the fraction used (arrow) for western blot analysis in (B) (WB). The high percentage of ECs was confirmed in the corresponding vWf immunofluorescent staining (IF), performed at P2 (200 cells were scored). (B) P2 ECs isolated with the recommended procedure from three patients: 8I, 1I (HD) and 10I and A, were lysed and analyzed for three EC and two mesenchymal markers by SDS PAGE. (C) Representative phase contrast (PC) images of the cells from the fractions selected for patient 10, stimulated or not by TGFbeta, before cell lysis and western blot analysis, are shown. The biochemical studies performed at P2 cross-validate the morphologic (performed at P0 and P1 stage by phase contrast imaging) and immunophenotypic (performed at P0 and P2 stage by immunofluorescent staining) characterization of the isolated cells.
From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. (2015)
Published customer image:
Sheep anti Human von Willebrand Factor antibody used for the evaluation of von Willebrand factor expression in isolated proliferating aortic cell preparations by immunofluorescence.
Image caption:
Analysis of proliferating cells: selection of EC-enriched fractions and validation of procedure.
(A) The left-hand morphologic diagram (M) shows the fraction used (arrow) for western blot analysis in (B) (WB). The high percentage of ECs was confirmed in the corresponding vWf immunofluorescent staining (IF), performed at P2 (200 cells were scored). (B) P2 ECs isolated with the recommended procedure from three patients: 8I, 1I (HD) and 10I and A, were lysed and analyzed for three EC and two mesenchymal markers by SDS PAGE. (C) Representative phase contrast (PC) images of the cells from the fractions selected for patient 10, stimulated or not by TGFbeta, before cell lysis and western blot analysis, are shown. The biochemical studies performed at P2 cross-validate the morphologic (performed at P0 and P1 stage by phase contrast imaging) and immunophenotypic (performed at P0 and P2 stage by immunofluorescent staining) characterization of the isolated cells.
From: Leclercq A, Veillat V, Loriot S, Spuul P, Madonna F, Roques X, et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS ONE 10(11): e0143144.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Human von Willebrand Factor antibody, used for the identification of von Willebrand expressing endothlial cells in the spinal cord of dark agouti rats with experimental acute encephalomyelitis by immunofluorescence.
Image caption:
SLPI expression within the spinal cord is associated with ED1-expressing macrophages or activated microglia in the acute phase (score 3.5, A) and with GFAP-positive astrocytes (B) and NeuN expressing neuronal cells (C) especially during the relapsing phase (score 3.5); there was no association of SLPI with von-Willebrand-factor positive endothelial cells (D). Magnification: 100x.
From: Mueller AM, Pedré X, Stempfl T, Kleiter I, Couillard-Despres S, Aigner L, Giegerich G, Steinbrecher A. Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
J Neuroinflammation. 2008 May 26;5:20.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti von Willebrand Factor antibody (AHP062) used for the evaluation of vWF expression on porcine endothelial cells by flow cytometry.
Image caption:
Identification of primary RMEC and AEC from GGTA1/CMAH DKO pigs (A) Expression of CD31, von Willebrand factor (vWF), SLA class I (SLAI), and SLA class II DR (SLA II DR) on DKO RMEC and AEC was confirmed by flow cytometry. (the ‘controls’are isotype controls)
From: Zhang J, Xie C, Lu Y, Zhou M, Qu Z, Yao D, Qiu C, Xu J, Pan D, Dai Y, Hara H, Cooper DKC, Ma S, Li M, Cai Z, Mou L.
Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model.
Sci Rep. 2017 Aug 30;7(1):10024.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Insert meta-description and applications
Image caption:
(A & C) Flow cytometric analysis of human fetal kidney cells (hFKC) grown in endothelial cell medium showing positive staining for endothelial cells marker vWF and progenitor endothelial cells markers Ulex Europaeus and CD133. (B & D) Flow cytometric analysis of hFKC grown in epithelial cell medium showing positive staining for epithelial cell markers CK8, CK18, ephrin receptors EPhA6 and EphA7 and stem cell markers DLK-1 and EPCAM.
From: Vijay Kumar Kuna, Sanchari Paul, Bo Xu, Robert Sjöback, Suchitra Sumitran-Holgersson,
Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney development
AIMS Cell and Tissue Engineering. (2019) 46: 07 Aug [Epub ahead of print].
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG was prepared from serum by ion exchange chromatography
- Antiserum Preparation
- Antisera to von Willebrand factor were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Benzamidine 0.1% EACA 1mM EDTA - Immunogen
- Purified human von Willebrand factor prepared from citrated human plasma
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04275
- Entrez Gene
- VWF
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0002020 protease binding
- GO:0002576 platelet degranulation
- GO:0051087 chaperone binding
- GO:0005783 endoplasmic reticulum
- GO:0005178 integrin binding
- GO:0005518 collagen binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0019865 immunoglobulin binding
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0031589 cell-substrate adhesion
- GO:0033093 Weibel-Palade body
- GO:0042803 protein homodimerization activity
- GO:0051260 protein homooligomerization
- GO:0047485 protein N-terminus binding
- Regulatory
- For research purposes only
Applications of von Willebrand Factor antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Flow Cytometry | |||
| Immunodiffusion | Neat | ||
| Immunofluorescence | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Tonsil
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for von Willebrand Factor antibody
-
Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.
J Immunol. 168 (9): 4308-17. -
Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22 (10): 839-49. -
Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
Mol Biol Cell. 13:1582-93. -
Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116: 2183-91. -
Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
J Biol Chem. 284: 12459-68. -
Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.
Clin Hemorheol Microcirc. 31: 287-93. -
Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
Exp Lung Res. 36: 1-11. -
Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22: 839-49. -
Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.
J Cell Sci. 123: 2964-75. -
Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
J Exp Med. 194: 797-808. -
Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
J Neuroinflammation. 5: 20. -
Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.
Am J Physiol Lung Cell Mol Physiol. 289: L196-206. -
Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
J Cell Sci. 126 (Pt 22): 5259-70. -
Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS One. 10 (11): e0143144. -
Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.
Proc Natl Acad Sci U S A. 112 (43): E5815-24. -
Zhang, J. et al. (2017) Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model.
Sci Rep. 7 (1): 10024. -
Kuna, V.K. et al. (2019) Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney development.
AIMS Cell and Tissue Engineering. 3 (1): 26-46.
