von Willebrand Factor antibody

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Sheep anti Human von Willebrand Factor:FITC

Sheep anti Human von Willebrand Factor

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP062F
Datasheet
C F* IF
MSDS
1 ml loader

AHP062T
Datasheet
C* E F IF
MSDS
0.1 ml loader

AHP062
Datasheet
C* E F ID IF
MSDS
1 ml loader

Sheep anti Human von Willebrand Factor antibody recognizes Human von Willebrand factor, a glycoprotein synthesized in endothelial cells and megakaryocytes and circulating in the blood as a noncovalent complex by association with factor VIII.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from serum.
Preparation
Purified IgG was prepared from serum by ion exchange chromatography
Antiserum Preparation
Antisera to von Willebrand factor were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
0.01%Benzamidine
0.1%EACA
1mMEDTA
Immunogen
Purified human von Willebrand factor prepared from citrated human plasma

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
18 months from date of despatch.

More Information

UniProt
P04275 Related reagents
Entrez Gene
VWF Related reagents
GO Terms
GO:0001948 glycoprotein binding
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0005783 endoplasmic reticulum
GO:0005178 integrin binding
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0007597 blood coagulation, intrinsic pathway
GO:0019865 immunoglobulin binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031589 cell-substrate adhesion
GO:0033093 Weibel-Palade body
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0047485 protein N-terminus binding
Regulatory
For research purposes only

Applications of von Willebrand Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Immunofluorescence
Immunohistology - Frozen 1/50 1/100
ELISA
Flow Cytometry
Immunodiffusion Neat
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil
Histology Positive Control Tissue
Tonsil

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

ELISA

Flow Cytometry

Immunofluorescence

Product Specific References

References for von Willebrand Factor antibody

  1. Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.
    J Immunol. 168 (9): 4308-17.
  2. Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
    Int Immunol. 22 (10): 839-49.
  3. Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
    Mol Biol Cell. 13:1582-93.
  4. Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
    Blood. 116: 2183-91.
  5. Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
    J Biol Chem. 284: 12459-68.
  6. Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.
    Clin Hemorheol Microcirc. 31: 287-93.
  7. Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
    Exp Lung Res. 36: 1-11.
  8. Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
    Int Immunol. 22: 839-49.
  9. Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.
    J Cell Sci. 123: 2964-75.
  10. Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
    J Exp Med. 194: 797-808.
  11. Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
    J Neuroinflammation. 5: 20.
  12. Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.
    Am J Physiol Lung Cell Mol Physiol. 289: L196-206.
  13. Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
    J Cell Sci. 126 (Pt 22): 5259-70.
  14. Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
    PLoS One. 10 (11): e0143144.
  15. Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.
    Proc Natl Acad Sci U S A. 112 (43): E5815-24.
  16. Zhang, J. et al. (2017) Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model.
    Sci Rep. 7 (1): 10024.
  17. Kuna, V.K. et al. (2019) Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney development.
    AIMS Cell and Tissue Engineering. 3 (1): 26-46.