von Willebrand Factor antibody





Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG was prepared from serum by ion exchange chromatography
- Antiserum Preparation
- Antisera to von Willebrand factor were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from serum by ion exchange chromatography
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.01% Benzamidine 0.1% EACA 1mM EDTA - Immunogen
- Purified human von Willebrand factor prepared from citrated human plasma
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04275
- Entrez Gene
- VWF
- GO Terms
- GO:0001948 glycoprotein binding
- GO:0002020 protease binding
- GO:0002576 platelet degranulation
- GO:0051087 chaperone binding
- GO:0005783 endoplasmic reticulum
- GO:0005178 integrin binding
- GO:0005518 collagen binding
- GO:0005578 proteinaceous extracellular matrix
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0019865 immunoglobulin binding
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- GO:0031589 cell-substrate adhesion
- GO:0033093 Weibel-Palade body
- GO:0042803 protein homodimerization activity
- GO:0051260 protein homooligomerization
- GO:0047485 protein N-terminus binding
- Regulatory
- For research purposes only
Applications of von Willebrand Factor antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Flow Cytometry | |||
Immunodiffusion | Neat | ||
Immunofluorescence | |||
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Histology Positive Control Tissue
- Tonsil
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
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Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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Product Specific References
References for von Willebrand Factor antibody
-
Blades, M.C. et al. (2002) Stromal cell-derived factor 1 (CXCL12) induces human cell migration into human lymph nodes transplanted into SCID mice.
J Immunol. 168 (9): 4308-17. -
Johnson, L.A. & Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22 (10): 839-49. -
Blagoveshchenskaya, A.D. et al. (2002) Selective and signal-dependent recruitment of membrane proteins to secretory granules formed by heterologously expressed von Willebrand factor.
Mol Biol Cell. 13:1582-93. -
Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116: 2183-91. -
Babich, V. et al. (2009) Differential effect of extracellular acidosis on the release and dispersal of soluble and membrane proteins secreted from the Weibel-Palade body.
J Biol Chem. 284: 12459-68. -
Butthep, P. et al. (2004) Endothelial injury and altered hemodynamics in thalassemia.
Clin Hemorheol Microcirc. 31: 287-93. -
Chowdhury, F. et al. (2010) Interactions between endothelial cells and epithelial cells in a combined cell model of airway mucosa: effects on tight junction permeability.
Exp Lung Res. 36: 1-11. -
Johnson, L.A. and Jackson, D.G. (2010) Inflammation-induced secretion of CCL21 in lymphatic endothelium is a key regulator of integrin-mediated dendritic cell transmigration.
Int Immunol. 22: 839-49. -
Kiskin, N.I. et al. (2010) Protein mobilities and P-selectin storage in Weibel-Palade bodies.
J Cell Sci. 123: 2964-75. -
Kriehuber, E. et al. (2001) Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.
J Exp Med. 194: 797-808. -
Mueller, A.M. et al. (2008) Novel role for SLPI in MOG-induced EAE revealed by spinal cord expression analysis.
J Neuroinflammation. 5: 20. -
Zhu, B. et al. (2005) Cyclic GMP-specific phosphodiesterase 5 regulates growth and apoptosis in pulmonary endothelial cells.
Am J Physiol Lung Cell Mol Physiol. 289: L196-206. -
Johnson, L.A. & Jackson, D.G. (2013) The chemokine CX3CL1 promotes trafficking of dendritic cells through inflamed lymphatics.
J Cell Sci. 126 (Pt 22): 5259-70. -
Leclercq, A. et al. (2015) A Methodology for Concomitant Isolation of Intimal and Adventitial Endothelial Cells from the Human Thoracic Aorta.
PLoS One. 10 (11): e0143144. -
Cheung, K. et al. (2015) CD31 signals confer immune privilege to the vascular endothelium.
Proc Natl Acad Sci U S A. 112 (43): E5815-24. -
Zhang, J. et al. (2017) Potential Antigens Involved in Delayed Xenograft Rejection in a Ggta1/Cmah Dko Pig-to-Monkey Model.
Sci Rep. 7 (1): 10024. -
Kuna, V.K. et al. (2019) Human fetal kidney cells regenerate acellular porcine kidneys via upregulation of key transcription factors involved in kidney development.
AIMS Cell and Tissue Engineering. 3 (1): 26-46.
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