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Tryptophan Hydroxylase antibody

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Anti Tryptophan Hydroxylase Antibody gallery image 1
Western blot of human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa Tryptophan hydroxylase protein using Sheep anti Human tryptophan hydroxylase antibody (AHP930).

Sheep anti Tryptophan Hydroxylase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP930
Datasheet
C WB
MSDS
0.1 ml loader

Sheep anti Human tryptophan hydroxylase antibody recognizes tryptophan hydroxylase (TPH) which catalyses the 5-hydroxylation of tryptophan, in the first stage of indoleamine biosynthesis. Serotonin biosynthesis occurs principally in neurons which originate in the raphe nucleus of the brain, and melatonin synthesis takes place within the pineal gland.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to tryptophan hydroxylase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
10mM HEPES pH7.5
Preservative Stabilisers
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol
Immunogen
Recombinant rabbit tryptophan hydroxylase purified from E. coli.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P17752
Entrez Gene
TPH1
GO Terms
GO:0004510 tryptophan 5-monooxygenase activity
GO:0005829 cytosol
GO:0005506 iron ion binding
GO:0055114 oxidation-reduction process
GO:0009072 aromatic amino acid family metabolic process
GO:0016597 amino acid binding
GO:0042427 serotonin biosynthetic process
GO:0042446 hormone biosynthetic process
Regulatory
For research purposes only

Applications of Tryptophan Hydroxylase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/1000
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP930 detects a band of approximately 55kDa in human dorsal Raphe nucleus lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA F IF 0.1 mg loader
Donkey anti Sheep/Goat IgG:FITC STAR88F C 1 mg loader
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader

Product Specific References

References for Tryptophan Hydroxylase antibody

  1. Haycock, J.W. et al. (2002) A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope.
    J Neurosci Methods. 114 (2): 205-12.
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