Transferrin antibody

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Sheep anti Human Transferrin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP081 ID IF 1 ml
Sheep anti Human transferrin antibody recognizes human transferrin, also known as beta-1 metal-binding globulin siderophilin or serotransferrin. Transferrin is 647 amino acid ~79 kDa iron binding transport glycoprotein involved in iron translocation from sites of degradation to those of of storage and utilization.

Mutations in the TF gene can lead to the iron overload disorder Atransferrinemia (ATRAF) a disease characterized abnormal transferrin production, iron overload and microcytic hypochromic anemia (Knisely et al. 2004).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Guinea Pig
Chicken
Mouse
Bovine
Rabbit
Pig
Rat
Dog
Horse
Cat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human transferrin were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%EACA
0.01%Benzamidine
1mMEDTA
Immunogen
Human holo-transferrin.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P02787 Related reagents
Entrez Gene
TF Related reagents
GO Terms
GO:0002576 platelet degranulation
GO:0005576 extracellular region
GO:0005769 early endosome
GO:0005770 late endosome
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0005905 coated pit
GO:0006879 cellular iron ion homeostasis
GO:0008199 ferric iron binding
GO:0009925 basal plasma membrane
GO:0010008 endosome membrane
GO:0016324 apical plasma membrane
GO:0030168 platelet activation
GO:0033572 transferrin transport
GO:0048471 perinuclear region of cytoplasm
GO:0055037 recycling endosome
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Transferrin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion Neat
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for Transferrin antibody

  1. Pak, S.C. et al. (1996) Super-CHO-A cell line capable of autocrine growth under fully defined protein-free conditions.
    Cytotechnology. 22 (1-3): 139-46.
  2. Kwon, E.E. & Prineas, J.W. (1994) Blood-brain barrier abnormalities in longstanding multiple sclerosis lesions. An immunohistochemical study.
    J Neuropathol Exp Neurol. 53 (6): 625-36.
  3. Sunstrom, N.A. et al. (2000) Regulated autocrine growth of CHO cells.
    Cytotechnology. 34 (1-2): 39-46.
  4. Navarro-garcía, F. et al. (2007) Intoxication of epithelial cells by plasmid-encoded toxin requires clathrin-mediated endocytosis.
    Microbiology. 153 (Pt 9): 2828-38.
  5. Tirosh, B. et al. (2009) Transferrin as a luminal target for negatively charged liposomes in the inflamed colonic mucosa.
    Mol Pharm. 6 (4): 1083-91.