Transferrin antibody
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Sheep anti Human transferrin antibody recognizes human transferrin, also known as beta-1 metal-binding globulin
siderophilin or serotransferrin. Transferrin is 647 amino acid ~79 kDa iron binding transport glycoprotein involved in iron translocation from sites of degradation to those of of storage and utilization.
Mutations in the TF gene can lead to the iron overload disorder Atransferrinemia (ATRAF) a disease characterized abnormal transferrin production, iron overload and microcytic hypochromic anemia (Knisely et al. 2004).
Mutations in the TF gene can lead to the iron overload disorder Atransferrinemia (ATRAF) a disease characterized abnormal transferrin production, iron overload and microcytic hypochromic anemia (Knisely et al. 2004).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Guinea Pig Chicken Mouse Bovine Rabbit Pig Rat Dog Horse Cat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human transferrin were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 0.1% EACA 0.01% Benzamidine 1mM EDTA - Immunogen
- Human holo-transferrin.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P02787
- Entrez Gene
- TF
- GO Terms
- GO:0002576 platelet degranulation
- GO:0005576 extracellular region
- GO:0005769 early endosome
- GO:0005770 late endosome
- GO:0030141 stored secretory granule
- GO:0030139 endocytic vesicle
- GO:0005905 coated pit
- GO:0006879 cellular iron ion homeostasis
- GO:0008199 ferric iron binding
- GO:0009925 basal plasma membrane
- GO:0010008 endosome membrane
- GO:0016324 apical plasma membrane
- GO:0030168 platelet activation
- GO:0033572 transferrin transport
- GO:0048471 perinuclear region of cytoplasm
- GO:0055037 recycling endosome
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of Transferrin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunodiffusion | Neat | ||
| Immunofluorescence |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Product Specific References
References for Transferrin antibody
-
Pak, S.C. et al. (1996) Super-CHO-A cell line capable of autocrine growth under fully defined protein-free conditions.
Cytotechnology. 22 (1-3): 139-46. -
Kwon, E.E. & Prineas, J.W. (1994) Blood-brain barrier abnormalities in longstanding multiple sclerosis lesions. An immunohistochemical study.
J Neuropathol Exp Neurol. 53 (6): 625-36. -
Sunstrom, N.A. et al. (2000) Regulated autocrine growth of CHO cells.
Cytotechnology. 34 (1-2): 39-46. -
Navarro-garcía, F. et al. (2007) Intoxication of epithelial cells by plasmid-encoded toxin requires clathrin-mediated endocytosis.
Microbiology. 153 (Pt 9): 2828-38. -
Tirosh, B. et al. (2009) Transferrin as a luminal target for negatively charged liposomes in the inflamed colonic mucosa.
Mol Pharm. 6 (4): 1083-91.
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