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TGN46 antibody

Product Code Applications Pack Size List Price Your Price Qty
AHP500GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * IF WB 10 µg loader
List Price Your Price
loader
AHP500G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * IF WB 25 µg loader
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loader

Sheep anti Human TGN46 antibody recognizes trans-Golgi network integral membrane protein 2 (TGOLN2), also known as TGN38 homolog, TGN46, TGN48 or trans-Golgi network protein TGN51. TGN46 is a 437 amino acid glycoprotein localized to the trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network.

TGN46 is a heavily glycosylated protein of around 110-120 kDa. Multiple isoforms of TGN46 are generated by alternative splicing differing in sequence at the C-terminal portion. Sheep anti Human TGN46 antibody is expected to recognize all identified isoforms.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human TGN46 were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
25% Glycerol
Immunogen
Recombinant human TGN46.
Approx. Protein Concentrations
IgG concentration 0.25 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence
Immunohistology - Frozen 1 0.1ug/ml 1ug/ml
Western Blotting 0.1ug/ml 1ug/ml
  1. 1Fixation with 3% paraformaldehyde or methanol/acetone is recommended.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):Biotin

References for TGN46 antibody

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  9. View The Latest Product References
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  34. Luo, S. et al. (2015) Contribution of N-linked glycans on HSV-2 gB to cell-cell fusion and viral entry.
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  37. Matrone, C. et al. (2016) Mannose 6-Phosphate Receptor Is Reduced in -Synuclein Overexpressing Models of Parkinsons Disease.
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Further Reading

  1. Ponnambalam, S. et al. (1996) Primate homologues of rat TGN38: primary structure, expression and functional implications.
    J Cell Sci. 109 ( Pt 3): 675-85.

Immunofluorescence

Western Blotting

Synonyms
TGOLN2
RRID
AB_323104
UniProt
O43493
Entrez Gene
TGOLN2

AHP500GT

148327 152174

AHP500G

150743 159576 164005 168412

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