TDP-43 antibody
Goat anti TDP-43
TDP-43 antibody recognizes TAR DNA-binding protein 43. This antibody has been extensively validated for western blotting using whole cell lysates.
TDP-43 Antibody with Control Lysate
TDP-43 antibody recognizes TAR DNA-binding protein 43. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00030 | WB | 100 µl | ||||
| VPA00030KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human TDP-43 antibody recognizes human TAR DNA-binding protein 43, also known as TDP-43, the product of the TAR DNA-binding protein 43 gene. TDP-43 is a 414 amino acid DNA and RNA binding protein containing 2 RRM domains involved in regulation of transcription and slpicing. TDP-43 is ubiquitouslyexpressed with high levels being seen in pancreas, gall bladder, alimentary tract, reproductive system, adpose tissue, skin and skeletal muscle.
Mutations in the TPD43 gene are implivated in development of Amyotrophic lateral sclerosis 10, a neurodegenerative disorder affecting motor neurons leading to eventual fatal paralysis (Van Deerlin et al. 2008).
Goat anti Human TDP-43 antibody recognizes human TDP-43 as a single band of ~45kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with rodent cell line lystaes tested.
Mutations in the TPD43 gene are implivated in development of Amyotrophic lateral sclerosis 10, a neurodegenerative disorder affecting motor neurons leading to eventual fatal paralysis (Van Deerlin et al. 2008).
Goat anti Human TDP-43 antibody recognizes human TDP-43 as a single band of ~45kDa in multiple cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with rodent cell line lystaes tested.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by ammonium sulfate precipitation followed by antigen affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide sequence C-HISNAEPKHNSNRQ from an internal region of TDP-43
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q13148 Related reagents
- Entrez Gene
- TARDBP Related reagents
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of TDP-43 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti TDP-43 antibody detects a band of approximately 45 kDa in NIH/3T3 cell lysates.
- Western Blotting
- Anti TDP-43 antibody detects a band of approximately 45 kDa in NIH/3T3 cell lysates.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
