RE1-SILENCING Transcription Factor antibody

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Goat anti RE1-SILENCING Transcription Factor

Goat anti-human RE1-silencing transcription factor antibody recognizes RE1-silencing transcription factor, also known as RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, or RE1-silencing transcription factor variant E1c/E2g/E3/E4.

RE1-SILENCING Transcription Factor Antibody with Control Lysate

Goat anti-human RE1-silencing transcription factor antibody recognizes RE1-silencing transcription factor, also known as RE1-silencing transcription factor variant E1a/E2/E3/N3a/E4i, or RE1-silencing transcription factor variant E1c/E2g/E3/E4.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00552 WB 100 µl
VPA00552KT WB 2 Westerns
With Lysate 400µg
Goat anti Human RE1-silencing transcription factor antibody recognizes the RE1-silencing transcription factor also known as REST, neural-restrictive silencer factor or X2 box repressor.

The REST gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described (provided by RefSeq, Jul 2010).

Goat anti Human RE1-silencing transcription factor antibody detects a band of 121 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti RE1-silencing transcription factor detects a band of approximately 121 kDa in Raji cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-PETPGAEDKGKSSK
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Positive Control Lysate

Lysate Composition
400μg Raji lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q13127 Related reagents
Entrez Gene
REST Related reagents
GO Terms
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0006350 transcription
GO:0008270 zinc ion binding
GO:0016564 transcription repressor activity
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of RE1-SILENCING Transcription Factor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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