NFkB p65 antibody
KO Validated
Sheep anti NFκB p65 antibody used for the detection of NFκB p65 expression in HEK293 cell lysates by western blotting
Image caption:
NF-κB p65 protein expression levels (a) Representative immunoblot membrane; (b) Combined densitometry data of three individual experiments, indicating fold changes in the protein expression of NF-κB p65, normalized to the total proteins transferred onto the membrane. BSA: bovine serum albumin; AGEs: advanced glycation end products;* p < 0.05; ** p < 0.01.
From: Serban, A.I.; Stanca, L.; Geicu, O.I.; Dinischiotu, A.
AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int. J. Mol. Sci. 2015, 16, 20100-20117.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Sheep anti NFκB antibody (VPA00015) used for the evaluation of NF&kappaB expression in normal human skin derived fibroblasts by western blotting.
Image caption:
AGEs induced signaling in CCD 1070SK fibroblasts. p-ERK1/2 and NF-κB p65 protein expression levels (a) Representative immunoblot membranes; (b) Combined densitometry data indicating fold changes in the protein expression of p-ERK1/2 (b) and NF-κB p65 (c). Immunoreactive bands were normalized to the total proteins transferred onto the membrane in the corresponding lane. Data are relative to BSA and nonimmune IgG treated cells. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01; *** p < 0.001.
From: Serban AI, Stanca L, Geicu OI, Munteanu MC, Dinischiotu A (2016)
RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS ONE 11(3): e0152376.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6.
Mouse anti NFkB p65 antibody was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System.
Sheep anti Human NFkB p65 antibody recognizes human full length human p65 (NFkB), also known as transcription factor p65 or nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. Human NFkB p65 is a 551 amino acid pleiotropic transcription factor containing a single RHD domain. Sheep anti Human NFkB p65 antibody will also detect proteolytic fragments of NFkB p65 containing the DNA binding RHD domain located in the amino-terminal region. Multiple isoforms of NFkB p65 are generated by alternative splicing. Sheep anti Human NFkB p65 antibody binds to isoforms 1 and 2 containing the intact RHD domain, but it is unclear whether it will bind to isoforms 3 and 4 which have partial differing deletions within the RHD domain.
Sheep anti Human NFkB p65 antibody recognizes human NFkB p65 as a single band of ~65 kDa in HEK293 cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.
Sheep anti Human NFkB p65 antibody recognizes human NFkB p65 as a single band of ~65 kDa in HEK293 cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.
Product Details
- Target Species
- Human
- Western Blotting
- Anti NFkB p65 antibody detects a band of approximately 65 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Sheep polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.25% Bovine Serum Albumin - Immunogen
- Human NFkB p65, amino acids 12-317, expressed in E. coli
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q04206
- Entrez Gene
- RELA
- GO Terms
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0003682 chromatin binding
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0005829 cytosol
- GO:0005667 transcription factor complex
- GO:0006916 anti-apoptosis
- GO:0006954 inflammatory response
- GO:0006968 cellular defense response
- GO:0008063 Toll signaling pathway
- GO:0042802 identical protein binding
- GO:0010224 response to UV-B
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016564 transcription repressor activity
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019901 protein kinase binding
- GO:0031293 membrane protein intracellular domain proteolysis
- GO:0031625 ubiquitin protein ligase binding
- GO:0032582 negative regulation of gene-specific transcription
- GO:0033613 activating transcription factor binding
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0042301 phosphate binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0047485 protein N-terminus binding
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050727 regulation of inflammatory response
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051607 defense response to virus
- GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
- GO:0070491 repressing transcription factor binding
- GO:0070555 response to interleukin-1
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of NFkB p65 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Gel Super Shift Assays | |||
| Immunoprecipitation | |||
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):HRP | 5184-2504 | C E WB | 2 mg |
|
Product Specific References
References for NFkB p65 antibody
-
Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int J Mol Sci. 16 (9): 20100-17. -
Serban, A.I. et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS One. 11 (3): e0152376.
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