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NFkB p65 antibody

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Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3 Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 4 Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 5 Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 6
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Published customer image:
Sheep anti NFκB p65 antibody used for the detection of NFκB p65 expression in HEK293 cell lysates by western blotting
Image caption:
NF-κB p65 protein expression levels (a) Representative immunoblot membrane; (b) Combined densitometry data of three individual experiments, indicating fold changes in the protein expression of NF-κB p65, normalized to the total proteins transferred onto the membrane. BSA: bovine serum albumin; AGEs: advanced glycation end products;* p < 0.05; ** p < 0.01.

From: Serban, A.I.; Stanca, L.; Geicu, O.I.; Dinischiotu, A.
AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
Int. J. Mol. Sci. 2015, 16, 20100-20117.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Published customer image:
Sheep anti NFκB antibody (VPA00015) used for the evaluation of NF&kappaB expression in normal human skin derived fibroblasts by western blotting.
Image caption:
AGEs induced signaling in CCD 1070SK fibroblasts. p-ERK1/2 and NF-κB p65 protein expression levels (a) Representative immunoblot membranes; (b) Combined densitometry data indicating fold changes in the protein expression of p-ERK1/2 (b) and NF-κB p65 (c). Immunoreactive bands were normalized to the total proteins transferred onto the membrane in the corresponding lane. Data are relative to BSA and nonimmune IgG treated cells. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01; *** p < 0.001.

From: Serban AI, Stanca L, Geicu OI, Munteanu MC, Dinischiotu A (2016)
RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
PLoS ONE 11(3): e0152376.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Western blot analysis of NFkB p65 CRISPR knockout HeLa (NFkB p65 KO) and wild type HeLa (WT) whole cell lysates probed with (A) Sheep anti NFkB p65 antibody (VPA00015) followed by detection with HRP conjugated Rabbit anti Sheep IgG (H/L) (1/10,000, 5184-2504) and (B) hFAB rhodamine anti-tubulin primary antibody (12004166) and visualized on the ChemiDoc MP with a (A) 27 second and (B) 30 second exposure.
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 4
Normalized signal intensity of NFkB p65 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 5
Western blot analysis of whole cell lysates probed with NFkB p65 antibody followed by detection with HRP conjugated Rabbit anti Sheep IgG (H/L) (1/10,000, 5184-2504) and visualized on the ChemiDoc MP with 23 second exposure. Arrow points to NFkB p65 (molecular weight 65 kDa).
Anti NFkB p65 Antibody (PrecisionAb Polyclonal Antibody) gallery image 6
Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 µg (lanes 2 & 7), 5 µg (lanes 3 & 8), 1 µg (lanes 4 & 9) (VPA00015), and 10 µg polyclonal IgG (lanes 5 & 10).

8.75 µl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 antibody was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc Touch Imaging System.

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Sheep anti NFkB p65

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
NFkB p65

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Product Code Applications Pack Size List Price Your Price Qty
VPA00015
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E GS IP WB 100 µl
List Price Your Price

Sheep anti Human NFkB p65 antibody recognizes human full length human p65 (NFkB), also known as transcription factor p65 or nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. Human NFkB p65 is a 551 amino acid pleiotropic transcription factor containing a single RHD domain. Sheep anti Human NFkB p65 antibody will also detect proteolytic fragments of NFkB p65 containing the DNA binding RHD domain located in the amino-terminal region. Multiple isoforms of NFkB p65 are generated by alternative splicing. Sheep anti Human NFkB p65 antibody binds to isoforms 1 and 2 containing the intact RHD domain, but it is unclear whether it will bind to isoforms 3 and 4 which have partial differing deletions within the RHD domain.

Sheep anti Human NFkB p65 antibody recognizes human NFkB p65 as a single band of ~65 kDa in HEK293 cell line lysates by western blotting under reducing conditions. It also cross reacts with rodent cell line lysates tested.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti NFkB p65 antibody detects a band of approximately 65 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Sheep polyclonal antibody purified by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.25% Bovine Serum Albumin
Immunogen
Human NFkB p65, amino acids 12-317, expressed in E. coli
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Gel Super Shift Assays
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):HRP 5184-2504 C E WB 2 mg loader
List Price Your Price
loader
Description Rabbit anti Sheep IgG (H/L):HRP

References for NFkB p65 antibody

  1. Serban, A.I. et al. (2015) AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism?
    Int J Mol Sci. 16 (9): 20100-17.
  2. Serban, A.I. et al. (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells.
    PLoS One. 11 (3): e0152376.

UniProt
Q04206
Entrez Gene
RELA
GO Terms
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0003682 chromatin binding
GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
GO:0005829 cytosol
GO:0005667 transcription factor complex
GO:0006916 anti-apoptosis
GO:0006954 inflammatory response
GO:0006968 cellular defense response
GO:0008063 Toll signaling pathway
GO:0042802 identical protein binding
GO:0010224 response to UV-B
GO:0010843 promoter binding
GO:0016563 transcription activator activity
GO:0016564 transcription repressor activity
GO:0019221 cytokine-mediated signaling pathway
GO:0019901 protein kinase binding
GO:0031293 membrane protein intracellular domain proteolysis
GO:0031625 ubiquitin protein ligase binding
GO:0032582 negative regulation of gene-specific transcription
GO:0033613 activating transcription factor binding
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0042301 phosphate binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0047485 protein N-terminus binding
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050727 regulation of inflammatory response
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051607 defense response to virus
GO:0070431 nucleotide-binding oligomerization domain containing 2 signaling pathway
GO:0070491 repressing transcription factor binding
GO:0070555 response to interleukin-1

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