NF Kappa B Inhibitor Alpha antibody

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Sheep anti Human NF Kappa B Inhibitor Alpha

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP395	E GS IP WB			0.1 mg

Summary
Secondary Antibodies

Sheep anti Human NF Kappa B Inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human NF kappa B inhibitor alpha was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.

Buffer Solution

TRIS buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
0.5%	Bovine Serum Albumin

Immunogen

Human NF kappa B inhibitor alpha expressed in E. coli.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P25963 Related reagents
Entrez Gene: NFKBIA Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0002755 MyD88-dependent toll-like receptor signaling pathway; GO:0002756 MyD88-independent toll-like receptor signaling pathway; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0005634 nucleus; GO:0006916 anti-apoptosis; GO:0007253 cytoplasmic sequestering of NF-kappaB; GO:0008063 Toll signaling pathway; GO:0008139 nuclear localization sequence binding; GO:0042802 identical protein binding; GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter; GO:0010745 negative regulation of macrophage derived foam cell differentiation; GO:0010875 positive regulation of cholesterol efflux; GO:0010888 negative regulation of lipid storage; GO:0031625 ubiquitin protein ligase binding; GO:0032088 negative regulation of NF-kappaB transcription factor activity; GO:0032270 positive regulation of cellular protein metabolic process; GO:0033256 I-kappaB/NF-kappaB complex; GO:0034130 toll-like receptor 1 signaling pathway; GO:0034134 toll-like receptor 2 signaling pathway; GO:0034138 toll-like receptor 3 signaling pathway; GO:0034142 toll-like receptor 4 signaling pathway; GO:0044419 interspecies interaction between organisms; GO:0045087 innate immune response; GO:0048011 nerve growth factor receptor signaling pathway; GO:0050852 T cell receptor signaling pathway; GO:0051059 NF-kappaB binding; GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory: For research purposes only

Applications of NF Kappa B Inhibitor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		1/2000
Gel Super Shift Assays
Immunoprecipitation
Western Blotting	1/200	1/1000

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Western Blotting: AHP395 detects a band of approximately 39 kDa in THPI cell lysates.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	1.5 ml	C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488	STAR88D488GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:DyLight®549	STAR88D549GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:DyLight®649	STAR88D649GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:FITC	STAR88F	1 mg	C
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

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