Sheep anti Human NF Kappa B Inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human NF kappa B inhibitor alpha was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
- Human NF kappa B inhibitor alpha expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
MyD88-dependent toll-like receptor signaling pathway
MyD88-independent toll-like receptor signaling pathway
cytoplasmic sequestering of NF-kappaB
Toll signaling pathway
nuclear localization sequence binding
identical protein binding
positive regulation of gene-specific transcription from RNA polymerase II promoter
negative regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol efflux
negative regulation of lipid storage
ubiquitin protein ligase binding
negative regulation of NF-kappaB transcription factor activity
positive regulation of cellular protein metabolic process
toll-like receptor 1 signaling pathway
toll-like receptor 2 signaling pathway
toll-like receptor 3 signaling pathway
toll-like receptor 4 signaling pathway
interspecies interaction between organisms
innate immune response
nerve growth factor receptor signaling pathway
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of NF Kappa B Inhibitor Alpha antibody
|Gel Super Shift Assays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP395 detects a band of approximately 39 kDa in THPI cell lysates.
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Secondary Antibodies Available
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