NF Kappa B Inhibitor Alpha antibody

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Sheep anti Human NF Kappa B Inhibitor Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP395 E GS IP WB 0.1 mg
Sheep anti Human NF Kappa B Inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human NF kappa B inhibitor alpha was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.5%Bovine Serum Albumin
Immunogen
Human NF kappa B inhibitor alpha expressed in E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P25963 Related reagents
Entrez Gene
NFKBIA Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0002755 MyD88-dependent toll-like receptor signaling pathway
GO:0002756 MyD88-independent toll-like receptor signaling pathway
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006916 anti-apoptosis
GO:0007253 cytoplasmic sequestering of NF-kappaB
GO:0008063 Toll signaling pathway
GO:0008139 nuclear localization sequence binding
GO:0042802 identical protein binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010745 negative regulation of macrophage derived foam cell differentiation
GO:0010875 positive regulation of cholesterol efflux
GO:0010888 negative regulation of lipid storage
GO:0031625 ubiquitin protein ligase binding
GO:0032088 negative regulation of NF-kappaB transcription factor activity
GO:0032270 positive regulation of cellular protein metabolic process
GO:0033256 I-kappaB/NF-kappaB complex
GO:0034130 toll-like receptor 1 signaling pathway
GO:0034134 toll-like receptor 2 signaling pathway
GO:0034138 toll-like receptor 3 signaling pathway
GO:0034142 toll-like receptor 4 signaling pathway
GO:0044419 interspecies interaction between organisms
GO:0045087 innate immune response
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050852 T cell receptor signaling pathway
GO:0051059 NF-kappaB binding
GO:0051092 positive regulation of NF-kappaB transcription factor activity
Regulatory
For research purposes only

Applications of NF Kappa B Inhibitor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Gel Super Shift Assays
Immunoprecipitation
Western Blotting 1/200 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP395 detects a band of approximately 39 kDa in THPI cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB