NF Kappa B Inhibitor Alpha antibody
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Sheep anti Human NF Kappa B Inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (NFkBIa, also known as IkB alpha). NFkBIa is one of seven members of the IkB class of inhibitor proteins that have been identified to date. NFkBIa inhibits the NFkB complex in unstimulated cells through non-covalent binding. Following stimulation of cells, NFkBIa is phosphorylated so that it dissociates from the NFkB and then ubiquinated prior to degradation.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human NF kappa B inhibitor alpha was raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Human NF kappa B inhibitor alpha expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P25963
- Entrez Gene
- NFKBIA
- GO Terms
- GO:0005886 plasma membrane
- GO:0002755 MyD88-dependent toll-like receptor signaling pathway
- GO:0002756 MyD88-independent toll-like receptor signaling pathway
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006916 anti-apoptosis
- GO:0007253 cytoplasmic sequestering of NF-kappaB
- GO:0008063 Toll signaling pathway
- GO:0008139 nuclear localization sequence binding
- GO:0042802 identical protein binding
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010745 negative regulation of macrophage derived foam cell differentiation
- GO:0010875 positive regulation of cholesterol efflux
- GO:0010888 negative regulation of lipid storage
- GO:0031625 ubiquitin protein ligase binding
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032270 positive regulation of cellular protein metabolic process
- GO:0033256 I-kappaB/NF-kappaB complex
- GO:0034130 toll-like receptor 1 signaling pathway
- GO:0034134 toll-like receptor 2 signaling pathway
- GO:0034138 toll-like receptor 3 signaling pathway
- GO:0034142 toll-like receptor 4 signaling pathway
- GO:0044419 interspecies interaction between organisms
- GO:0045087 innate immune response
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- Regulatory
- For research purposes only
Applications of NF Kappa B Inhibitor Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | ||
| Gel Super Shift Assays | |||
| Immunoprecipitation | |||
| Western Blotting | 1/200 | 1/1000 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP395 detects a band of approximately 39 kDa in THPI cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
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