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MTHFD1L antibody

Goat anti MTHFD1L

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
MTHFD1L

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VPA00159
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Goat anti Human MTHFL1 antibody recognizes MTHFL1, also known as monofunctional C1-tetrahydrofolate synthase and mitochondrial formyltetrahydrofolate synthetase.

MTHFD1L is involved in the synthesis of tetrahydrofolate (THF) in mitochondria. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for MTHFD1L (provided by RefSeq, Jun 2011).

Goat anti Human MTHFD1L antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti MTHFD1L detects a band of approximately 105 kDa in Jurkat cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5% BSA
Immunogen
Peptide with the sequence C-DTNDRFLRKITIGQ, from the internal region of the protein sequence
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
Q6UB35
Entrez Gene
MTHFD1L
GO Terms
GO:0005739 mitochondrion
GO:0004329 formate-tetrahydrofolate ligase activity
GO:0005524 ATP binding
GO:0006730 one-carbon metabolic process
GO:0009396 folic acid-containing compound biosynthetic process
GO:0015942 formate metabolic process
GO:0042803 protein homodimerization activity
GO:0046653 tetrahydrofolate metabolic process

VPA00159

060315

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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