LRRK2 antibody

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Goat anti LRRK2

LRRK2 antibody recognizes leucine-rich repeat serine/threonine-protein kinase 2, also known as dardarin. The antibody has been extensively validated for western blotting using whole cell lysates.

LRRK2 Antibody with Control Lysate

LRRK2 antibody recognizes leucine-rich repeat serine/threonine-protein kinase 2, also known as dardarin. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00118 WB 100 µl
VPA00118KT WB 2 Westerns
With Lysate 400µg
Goat anti Human LRRK2 antibody recognizes leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2), also known as dardarin.

LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in LRRK2 have been associated with Parkinson disease-8 (provided by RefSeq, Jul 2008).

Goat anti Human LRRK2 antibody detects a band of 286 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence CELAEKMRRTSV, from the internal region of the protein sequence.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q5S007 Related reagents
Entrez Gene
LRRK2 Related reagents
GO Terms
GO:0000186 activation of MAPKK activity
GO:0004708 MAP kinase kinase activity
GO:0005096 GTPase activator activity
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0006979 response to oxidative stress
GO:0007264 small GTPase mediated signal transduction
GO:0007528 neuromuscular junction development
GO:0008340 determination of adult lifespan
GO:0018105 peptidyl-serine phosphorylation
GO:0031398 positive regulation of protein ubiquitination
GO:0032473 external side of mitochondrial outer membrane
GO:0034211 GTP-dependent protein kinase activity
GO:0040012 regulation of locomotion
GO:0042803 protein homodimerization activity
GO:0043068 positive regulation of programmed cell death
GO:0046777 protein autophosphorylation
GO:0070997 neuron death
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of LRRK2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti LRRK2 detects a band of approximately 286 kDa in HeLa cell lysates.
Western Blotting
Anti LRRK2 detects a band of approximately 286 kDa in HeLa cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
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