The protein encoded by the LDHA gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA causes glycogen storage disease 11 (GSD11), a condition characterized by exertional myoglobinuria, pain, cramps and easy fatigue (Kanno et al. 1988). Multiple transcript variants encoding different isoforms have been found for LDHA. The human genome contains several non-transcribed pseudogenes of LDHA (provided by RefSeq, Sep 2008).
Goat anti Human LDHA antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
- Target Species
- Western Blotting
- Anti LDHA detects a band of approximately 33 kDa in HeLa cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
- Peptide sequence C-SDLVKVTLTSE
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0004459 L-lactate dehydrogenase activity
- GO:0005829 cytosol
- GO:0006090 pyruvate metabolic process
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- PrecisionAb is a trademark of Bio-Rad Laboratories
- For research purposes only.
Applications of Ldha antibody
|Application Name||Verified||Min Dilution||Max Dilution|
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||C E P WB||1 ml|