Ldha antibody

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Goat anti Ldha

Goat anti-human LDHA antibody recognizes L-lactate dehydrogenase A chain, also known as LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P or renal carcinoma antigen NY-REN-59.

Ldha Antibody with Control Lysate

Goat anti-human LDHA antibody recognizes L-lactate dehydrogenase A chain, also known as LDH muscle subunit, LDH-A, LDH-M, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P or renal carcinoma antigen NY-REN-59.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00516 WB 100 µl
VPA00516KT WB 2 Westerns
With Lysate 400µg
Goat anti Human LDHA antibody recognizes L-lactate dehydrogenase A chain, also known as LDH muscle subunit, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P, lactate dehydrogenase M or renal carcinoma antigen NY-REN-59.

The protein encoded by the LDHA gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA causes glycogen storage disease 11 (GSD11), a condition characterized by exertional myoglobinuria, pain, cramps and easy fatigue (Kanno et al. 1988). Multiple transcript variants encoding different isoforms have been found for LDHA. The human genome contains several non-transcribed pseudogenes of LDHA (provided by RefSeq, Sep 2008).

Goat anti Human LDHA antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti LDHA detects a band of approximately 33 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-SDLVKVTLTSE
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P00338 Related reagents
Entrez Gene
LDHA Related reagents
GO Terms
GO:0005515 protein binding
GO:0004459 L-lactate dehydrogenase activity
GO:0005829 cytosol
GO:0006090 pyruvate metabolic process
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Ldha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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