Ldha antibody

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Goat anti Ldha

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00516
Datasheet
WB
SDS
100 µl

Goat anti Human LDHA antibody recognizes L-lactate dehydrogenase A chain, also known as LDH muscle subunit, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P, lactate dehydrogenase M or renal carcinoma antigen NY-REN-59.

The protein encoded by the LDHA gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA causes glycogen storage disease 11 (GSD11), a condition characterized by exertional myoglobinuria, pain, cramps and easy fatigue (Kanno et al. 1988). Multiple transcript variants encoding different isoforms have been found for LDHA. The human genome contains several non-transcribed pseudogenes of LDHA (provided by RefSeq, Sep 2008).

Goat anti Human LDHA antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti LDHA detects a band of approximately 33 kDa in HeLa cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-SDLVKVTLTSE

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P00338
Entrez Gene
LDHA
GO Terms
GO:0005515 protein binding
GO:0004459 L-lactate dehydrogenase activity
GO:0005829 cytosol
GO:0006090 pyruvate metabolic process
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of Ldha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader