LDHA antibody
KO Validated
Filter by Application:
WB ResetGoat anti LDHA
- Product Type
- PrecisionAb Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
- Specificity
- LDHA
Antibody Quality and Performance Guarantee
| Goat anti Human LDHA antibody recognizes L-lactate dehydrogenase A chain, also known as LDH muscle subunit, cell proliferation-inducing gene 19 protein, epididymis secretory sperm binding protein Li 133P, lactate dehydrogenase M or renal carcinoma antigen NY-REN-59. The protein encoded by the LDHA gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA causes glycogen storage disease 11 (GSD11), a condition characterized by exertional myoglobinuria, pain, cramps and easy fatigue (Kanno et al. 1988). Multiple transcript variants encoding different isoforms have been found for LDHA. The human genome contains several non-transcribed pseudogenes of LDHA (provided by RefSeq, Sep 2008). Goat anti Human LDHA antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti LDHA detects a band of approximately 33 kDa in HeLa cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid.
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Buffer Solution
- TRIS buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin. - Immunogen
- Peptide sequence C-SDLVKVTLTSE
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
- UniProt
- P00338
- Entrez Gene
- LDHA
- GO Terms
- GO:0005515 protein binding
- GO:0004459 L-lactate dehydrogenase activity
- GO:0005829 cytosol
- GO:0006090 pyruvate metabolic process
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
VPA00516
160712
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