Indoleamine 2,3-Dioxygenase antibody

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Sheep anti Human Indoleamine 2,3-Dioxygenase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP833 P* WB 0.1 ml
Sheep anti Human Indoleamine 2,3-Dioxygenase antibody recognizes human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Marmoset
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig Fraction - liquid
Antiserum Preparation
Antisera to human indoleamine dioxygenase (IDO) were raised by repeated immunisation of sheep with highly purified antigen. Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Recombinant human indoleamine 2,3-dioxygenase.
Approx. Protein Concentrations
Total protein concentration 27.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P14902 Related reagents
Entrez Gene
IDO1 Related reagents
GO Terms
GO:0009055 electron carrier activity
GO:0005829 cytosol
GO:0004833 tryptophan 2,3-dioxygenase activity
GO:0055114 oxidation-reduction process
GO:0006569 tryptophan catabolic process
GO:0007565 female pregnancy
GO:0020037 heme binding
GO:0033754 indoleamine 2,3-dioxygenase activity
Regulatory
For research purposes only

Applications of Indoleamine 2,3-Dioxygenase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Indoleamine 2,3-Dioxygenase antibody

  1. Genescà, M. et al. (2012) Live-attenuated lentivirus immunization modulates innate immunity and inflammation while protecting rhesus macaques from vaginal simian immunodeficiency virus challenge.
    J Virol. 86 (17): 9188-200.
  2. Drenzek, J.G. et al. (2008) Expression of indoleamine 2,3-dioxygenase in the rhesus monkey and common marmoset.
    J Reprod Immunol. 78 (2): 125-33.
  3. von Bergwelt-Baildon, M.S. et al. (2006) CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms of T-cell inhibition.
    Blood. 108: 228-37.
  4. Scheler, M. et al. (2007) Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
    Am J Pathol. 171: 1936-43.
  5. Popov, A. et al. (2006) Indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection.
    J Clin Invest. 116: 3160-70.
  6. Torres, M.I. et al. (2007) Tryptophan metabolism and indoleamine 2,3-dioxygenase expression in coeliac disease.
    Clin Exp Immunol. 148: 419-24.
  7. Popov, A. et al. (2008) Infection of myeloid dendritic cells with Listeria monocytogenes leads to the suppression of T cell function by multiple inhibitory mechanisms.
    J Immunol. 181: 4976-88.
  8. von Bubnoff, D. et al. (2012) Indoleamine 2,3-dioxygenase expression in early keratocyte neoplasia of the lower lip correlates to the degree of cell atypia.
    Pathol Int. 62: 105-11.
  9. von Bubnoff, D. et al. (2011) Indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells and macrophages in infectious and noninfectious cutaneous granulomas.
    J Am Acad Dermatol. 65 (4): 819-32.

Further Reading

  1. Munn, D.H. & Mellor, A.L. (2004) IDO and tolerance to tumors.
    Trends Mol Med. 10 (1): 15-8.