Indoleamine 2,3-Dioxygenase antibody

Sheep anti Human Indoleamine 2,3-Dioxygenase

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Indoleamine 2,3-Dioxygenase

Product Code Applications Pack Size List Price Your Price Qty
AHP833
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 ml
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Sheep anti Human Indoleamine 2,3-Dioxygenase antibody recognizes human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Marmoset
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Ig Fraction - liquid
Antiserum Preparation
Antisera to human indoleamine dioxygenase (IDO) were raised by repeated immunisation of sheep with highly purified antigen. Ig fraction prepared by ammonium sulphate precipitation.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant human indoleamine 2,3-dioxygenase.
Approx. Protein Concentrations
Total protein concentration 27.1 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Indoleamine 2,3-Dioxygenase antibody

  1. Genescà, M. et al. (2012) Live-attenuated lentivirus immunization modulates innate immunity and inflammation while protecting rhesus macaques from vaginal simian immunodeficiency virus challenge.
    J Virol. 86 (17): 9188-200.
  2. Drenzek, J.G. et al. (2008) Expression of indoleamine 2,3-dioxygenase in the rhesus monkey and common marmoset.
    J Reprod Immunol. 78 (2): 125-33.
  3. von Bergwelt-Baildon, M.S. et al. (2006) CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms of T-cell inhibition.
    Blood. 108: 228-37.
  4. Scheler, M. et al. (2007) Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
    Am J Pathol. 171: 1936-43.
  5. Popov, A. et al. (2006) Indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection.
    J Clin Invest. 116: 3160-70.
  6. Torres, M.I. et al. (2007) Tryptophan metabolism and indoleamine 2,3-dioxygenase expression in coeliac disease.
    Clin Exp Immunol. 148: 419-24.
  7. Popov, A. et al. (2008) Infection of myeloid dendritic cells with Listeria monocytogenes leads to the suppression of T cell function by multiple inhibitory mechanisms.
    J Immunol. 181: 4976-88.
  8. von Bubnoff, D. et al. (2012) Indoleamine 2,3-dioxygenase expression in early keratocyte neoplasia of the lower lip correlates to the degree of cell atypia.
    Pathol Int. 62: 105-11.
  9. View The Latest Product References
  10. von Bubnoff, D. et al. (2011) Indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells and macrophages in infectious and noninfectious cutaneous granulomas.
    J Am Acad Dermatol. 65 (4): 819-32.

Further Reading

  1. Munn, D.H. & Mellor, A.L. (2004) IDO and tolerance to tumors.
    Trends Mol Med. 10 (1): 15-8.

Immunofluorescence

Immunohistology - Paraffin

Western Blotting

Synonyms
IDO
RRID
AB_2123541
UniProt
P14902
Entrez Gene
IDO1
GO Terms
GO:0009055 electron carrier activity
GO:0005829 cytosol
GO:0004833 tryptophan 2,3-dioxygenase activity
GO:0055114 oxidation-reduction process
GO:0006569 tryptophan catabolic process
GO:0007565 female pregnancy
GO:0020037 heme binding
GO:0033754 indoleamine 2,3-dioxygenase activity