Sheep anti Human Indoleamine 2,3-Dioxygenase antibody recognizes human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Ig Fraction - liquid
- Antiserum Preparation
- Antisera to human indoleamine dioxygenase (IDO) were raised by repeated immunisation of sheep with highly purified antigen. Ig fraction prepared by ammonium sulphate precipitation.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Recombinant human indoleamine 2,3-dioxygenase.
- Approx. Protein Concentrations
- Total protein concentration 27.1 mg/ml
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
electron carrier activity
tryptophan 2,3-dioxygenase activity
tryptophan catabolic process
indoleamine 2,3-dioxygenase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Indoleamine 2,3-Dioxygenase antibody
|Immunohistology - Frozen
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Indoleamine 2,3-Dioxygenase antibody
Genescà, M. et al. (2012) Live-attenuated lentivirus immunization modulates innate immunity and inflammation while protecting rhesus macaques from vaginal simian immunodeficiency virus challenge.
J Virol. 86 (17): 9188-200.
Drenzek, J.G. et al. (2008) Expression of indoleamine 2,3-dioxygenase in the rhesus monkey and common marmoset.
J Reprod Immunol. 78 (2): 125-33.
von Bergwelt-Baildon, M.S. et al. (2006) CD25 and indoleamine 2,3-dioxygenase are up-regulated by prostaglandin E2 and expressed by tumor-associated dendritic cells in vivo: additional mechanisms of T-cell inhibition.
Blood. 108: 228-37.
Scheler, M. et al. (2007) Indoleamine 2,3-dioxygenase (IDO): the antagonist of type I interferon-driven skin inflammation?
Am J Pathol. 171: 1936-43.
Popov, A. et al. (2006) Indoleamine 2,3-dioxygenase-expressing dendritic cells form suppurative granulomas following Listeria monocytogenes infection.
J Clin Invest. 116: 3160-70.
Torres, M.I. et al. (2007) Tryptophan metabolism and indoleamine 2,3-dioxygenase expression in coeliac disease.
Clin Exp Immunol. 148: 419-24.
Popov, A. et al. (2008) Infection of myeloid dendritic cells with Listeria monocytogenes leads to the suppression of T cell function by multiple inhibitory mechanisms.
J Immunol. 181: 4976-88.
von Bubnoff, D. et al. (2012) Indoleamine 2,3-dioxygenase expression in early keratocyte neoplasia of the lower lip correlates to the degree of cell atypia.
Pathol Int. 62: 105-11.
von Bubnoff, D. et al. (2011) Indoleamine 2,3-dioxygenase-expressing myeloid dendritic cells and macrophages in infectious and noninfectious cutaneous granulomas.
J Am Acad Dermatol. 65 (4): 819-32.
Munn, D.H. & Mellor, A.L. (2004) IDO and tolerance to tumors.
Trends Mol Med. 10 (1): 15-8.
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