IL-7 antibody
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Rabbit anti Human Interleukin-7
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-7
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-7 antibody recognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways. |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-7 (PHP046).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.5 | 2.0 ug/ml |
| Functional Assays | |
0.05 | 0.08ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- Rabbit anti Human IL-7 (purified) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (biotin conjugate) as the detection reagent.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (Fc):Biotin | ||||||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (Fc):FITC | ||||||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (Fc):HRP | ||||||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Goat anti Rabbit IgG (H/L):HRP | ||||||
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Sheep anti Rabbit IgG:FITC | ||||||
| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Sheep anti Rabbit IgG:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
References for IL-7 antibody
-
Lupton, S.D. et al. (1990) Characterization of the human and murine IL-7 genes.
J Immunol. 144 (9): 3592-601. -
Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.
Blood. 99 (11): 3892-904.
- RRID
- AB_323578
- UniProt
- P13232
- Entrez Gene
- IL7
- GO Terms
- GO:0005125 cytokine activity
- GO:0005139 interleukin-7 receptor binding
- GO:0005615 extracellular space
- GO:0006959 humoral immune response
- GO:0007267 cell-cell signaling
- GO:0009887 organ morphogenesis
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0045453 bone resorption
- View More GO Terms
- GO:0045582 positive regulation of T cell differentiation
AHP283G
146307
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