IL-7 antibody

Rabbit anti Human Interleukin-7

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-7

Product Code Applications Pack Size List Price Your Price Qty
AHP283G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rabbit anti Human Interleukin-7 antibody recognizes human interleukin-7. IL-7 is a 152 amino acid glycoprotein and pleiotropic haematopoietic growth factor, secreted by thymic, splenic and bone marrow stromal/epithelial cells, keratinocytes and follicular dendritic cells. Originally termed pre-B cell factor, due to its involvement in the development of early B cells, IL-7 has since been shown to be mitogenic for early T cells and can also co-stimulate the proliferation of mature T cells in the presence of IL-2 and Concavalin A. IL-7 signals through a transmembrane receptor consisting of the IL-7Ra chain (CD127) and common cytokine gamma chain, triggering a number of non-receptor tyrosine kinase signalling pathways.

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antiserum Preparation
Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human IL-7 (PHP046).
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0 ug/ml
Functional Assays 0.05 0.08ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
Rabbit anti Human IL-7 (purified) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (biotin conjugate) as the detection reagent.

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References for IL-7 antibody

  1. Lupton, S.D. et al. (1990) Characterization of the human and murine IL-7 genes.
    J Immunol. 144 (9): 3592-601.
  2. Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.
    Blood. 99 (11): 3892-904.

RRID
AB_323578
UniProt
P13232
Entrez Gene
IL7
GO Terms
GO:0005125 cytokine activity
GO:0005139 interleukin-7 receptor binding
GO:0005615 extracellular space
GO:0006959 humoral immune response
GO:0007267 cell-cell signaling
GO:0009887 organ morphogenesis
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0045453 bone resorption
GO:0045582 positive regulation of T cell differentiation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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