IL-7 antibody

Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Product Form
- Purified IgG - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. - Reconstitution
- Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
- Antiserum Preparation
- Antisera to human IL-7 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-7 (PHP046).
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml after reconstitution
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
Storage Information
- Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 6 months from date of despatch
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
More Information
- UniProt
- P13232
- Entrez Gene
- IL7
- GO Terms
- GO:0005125 cytokine activity
- GO:0005139 interleukin-7 receptor binding
- GO:0005615 extracellular space
- GO:0006959 humoral immune response
- GO:0007267 cell-cell signaling
- GO:0009887 organ morphogenesis
- GO:0008083 growth factor activity
- GO:0030890 positive regulation of B cell proliferation
- GO:0045453 bone resorption
- GO:0045582 positive regulation of T cell differentiation
- Regulatory
- For research purposes only
Applications of IL-7 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.15 | 0.30ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml | |
ELISA | 0.5 | 2.0 ug/ml | |
Functional Assays | 0.05 | 0.08ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- Rabbit anti Human IL-7 (biotin conjugate) may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (purified) as the capture reagent.
- ELISA
- Rabbit anti Human IL-7 (purified) may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with recombinant human IL-7 as the standard and Rabbit anti Human IL-7 (biotin conjugate) as the detection reagent.
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for IL-7 antibody
-
Lupton, S.D. et al. (1990) Characterization of the human and murine IL-7 genes.
J Immunol. 144 (9): 3592-601. -
Fry, T.J. & Mackall, C.L. (2002) Interleukin-7: from bench to clinic.
Blood. 99 (11): 3892-904.
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