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IL-7

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Sandwich ELISA analysis of human interleukin-7 using Rat anti Human IL-7 (1012201) as a capture reagent and biotinylated Rat anti Human IL-7 antibody as a detection reagent with recombinant human IL-7 (PHP046) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). A human serum sample is indicated (green).

Recombinant Human Interleukin-7

Product Type
Recombinant Protein
Specificity
IL-7

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Product Code Applications Pack Size List Price Your Price Qty
PHP046
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SDS Safety Datasheet SDS
E FN WB 10 µg loader
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Recombinant human IL-7 is a 17.4 kDa protein comprised of 153 amino acids corresponing to the ful-length mature sequence.

IL-7 is a potent lymphoid cell growth factor acting on Pre-B, Pro-B and early T cells. In a dose dependent cell proliferation assay using 2E8 cells this product shows a specific activity of > 2 x 106 units/mg.

Recombinant human IL-7 is recognized by Rat anti Human IL-7 antibody clone BVD10-40F6 in ELISA.

Target Species
Human
Product Form
Purified recombinant protein expressed in E. coli - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Source
E.coli
Buffer Solution
10mM Acetic Acid
Preservative Stabilisers
None present
Carrier Free
Yes
Purity
>98 % by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 0.1 mg/ml after reconstitution
Protein Molecular Weight
17.5kDa (153 amino acids)
Endotoxin Level
< 0.1 ng/ug
Regulatory
For research purposes only
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

Prior to reconstitution store at -20oC. Following reconstitution store at -20oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.2 0.4 ng/well
Functional Assays 0.1ng/ml 10ng/ml
Western Blotting 1.5 3.0 ng/lane
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate negative/positive controls.
ELISA
Recombinant human IL-7 may be used as a standard for ELISA applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).
Western Blotting
Recombinant human IL-7 may be used as the positive control for western blotting applications with either anti-Human IL-7 (purified) or anti-Human IL-7 (biotin conjugate).

UniProt
P13232
Entrez Gene
IL7
GO Terms
GO:0005125 cytokine activity
GO:0005139 interleukin-7 receptor binding
GO:0005615 extracellular space
GO:0006959 humoral immune response
GO:0007267 cell-cell signaling
GO:0009887 organ morphogenesis
GO:0008083 growth factor activity
GO:0030890 positive regulation of B cell proliferation
GO:0045453 bone resorption
GO:0045582 positive regulation of T cell differentiation

PHP046

155864 161387 166193

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