IgM antibody

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Goat anti Human IgM:Alk. Phos.

Goat anti Human IgM:Biotin

Goat anti Human IgM:RPE

Goat anti Human IgM:TRITC

Goat anti Human IgM:Texas Red®

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
202004 C E IB 1 ml
202008 E F IB 1 mg
202009 F 0.5 mg
202003 C F 1 mg
202007 F 1 mg
Goat anti human IgM polyclonal antibody reacts with the heavy chain of human IgM. This antibody has been cross-absorbed to reduce cross-reactivity with human IgG and IgA, but may react with IgM from other species.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Product Form
Purified IgG conjugated to Rhodamine (TRITC) - liquid
Product Form
Purified IgG conjugated to Texas Red® (TXRD) - Liquid
Antiserum Preparation
Antiserum to human IgM was raised by repeated immunisation of goats with human IgM paraproteins. The preparation was purified by affinity chromatography on human IgM covalently linked to Sepharose-4B.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide
Stabilising agent
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Human IgM
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from the date of despatch.
Shelf Life
Please see label for expiry date

More Information

UniProt
P01871 Related reagents
Entrez Gene
IGHM Related reagents
GO Terms
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunoblotting
Immunohistology - Frozen
ELISA 1/5,000 1/20,000
Flow Cytometry Neat 1/10
Immunoblotting
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Immunohistology - Frozen
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F