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IgG antibody

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F(ab')2 anti Human IgM Antibody - FITC thumbnail image 1
F(ab')2 anti Human IgM Antibody - FITC gallery image 1
Published customer image:
Goat F(ab′)2 anti Human IgG antibody (STAR97P) used for the detection of RA serum samples on citrullinated targets by western blotting.
Image caption:
Citrullination facilitates cross-reactivity of RF+ serum with fibrinogen in the absence of ACPAs.
(b) Native or citrullinated fibrinogen was probed using sera from the indicated RA patient cohorts or healthy controls.

From: Trela M, Perera S, Sheeran T, Rylance P, Nelson PN, Attridge K.
Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.
Sci Rep. 2019 Aug 19;9(1):12068.
doi: 10.1038/s41598-019-48176-3.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat F(ab')2 anti Human IgG:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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Product Code Applications Pack Size List Price Your Price Qty
STAR97P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.6 mg loader
List Price Your Price
loader

Goat F(ab′)2 anti Human IgG antibody recognizes human IgG and demonstrates <1% cross-reactivity with other human immunoglobulin classes or subclasses.

Goat F(ab′)2 anti Human IgG antibody does not cross-react with Goat IgG F(c) or mouse, rat, bovine and horse serum proteins, as determined by immunoelectrophoresis.

Goat F(ab′)2 anti Human IgG antibody has been used for the detection of the antibody reponse to an endogenous retrovirus GAG matrix in patients with rheumatoid arthritis by ELISA (Nelson et al. 2014).

Target Species
Human
Product Form
F(ab')2 fragments of purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to human IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Human IgG.
Approx. Protein Concentrations
IgG concentration 0.6 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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TMB Core BUF056A E 100 ml
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TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for IgG antibody

  1. Nelson, P.N. et al. (2014) Rheumatoid Arthritis is Associated with IgG Antibodies to Human Endogenous Retrovirus Gag Matrix: A Potential Pathogenic Mechanism of Disease?
    J Rheumatol. 41: 1952-60.
  2. Trela, M. et al. (2019) Citrullination facilitates cross-reactivity of rheumatoid factor with non-IgG1 Fc epitopes in rheumatoid arthritis.
    Sci Rep. 9 (1): 12068.

RRID
AB_567031
UniProt
P01857
P01834
P01860
P01859
P01861
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response

STAR97P

155994 157485 158132 1707

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