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IgG antibody

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Goat anti Human IgG F(ab')2:Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
F(ab')2

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Goat anti Human IgG F(ab')2 antibody will recognize both the heavy and light chains of the F(ab')2 portion of human IgG by IEP. This product will also react with light chains from other human immunoglobulin types. This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.

This peroxidase-labelled antibody is a performance tested, matched reagent for HuCAL antibodies.

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin
Immunogen
Human serum.
Approx. Protein Concentrations
IgG concentration 0.6 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Stable for 6 weeks at 4ºC as an undiluted liquid. For longer term storage (>6 weeks) please add an equal volume of glycerol and store at -20ºC. Avoid freeze-thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1,000 1/10,000
Western Blotting 1/1,000 1/10,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Ohashi, M. et al. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
    J Biol Chem. 285 (22): 16408-15.
  2. Wang, Y. et al. (2011) Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures.
    Mol Pharm. 8 (4): 1016-24.
  3. Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
    Protein Eng Des Sel. 25 (5): 251-9.
  4. Thiede-Stan, N.K. et al. (2015) Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.
    J Cell Sci. 128 (19): 3583-96.
  5. Mukbel, R.M. et al. (2016) Human immune response to salivary proteins of wild-caught Phlebotomus papatasi.
    Parasitol Res. 115 (9): 3345-55.

RRID
AB_616682
UniProt
P01857
P01861
P01834
P01859
P01860
P0CG04
Entrez Gene
IGKC
IGHG1
IGHG2
IGHG3
IGHG4
IGLV2-14
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response

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