IgG antibody

This peroxidase-labelled antibody is a performance tested, matched reagent for HuCAL antibodies.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin - Preservative Stabilisers
0.01% Thiomersal 1% Bovine Serum Albumin - Immunogen
- Human serum.
- Approx. Protein Concentrations
- IgG concentration 0.6 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Stable for 6 weeks at 4ºC as an undiluted liquid. For longer term storage (>6 weeks) please add an equal volume of glycerol and store at -20ºC. Avoid freeze-thaw cycles.
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01857
- P01861
- P01834
- P01859
- P01860
- P0CG04
- Entrez Gene
- IGKC
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGLV2-14
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/1,000 | 1/10,000 | |
Western Blotting | 1/1,000 | 1/10,000 | |
ELISA | 1/5,000 | 1/100,000 | |
Western Blotting | 1/10,000 | 1/200,000 |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Wang, Y. et al. (2011) Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures.
Mol Pharm. 8 (4): 1016-24. -
Ohashi, M. et al. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
J Biol Chem. 285 (22): 16408-15. -
Thiede-Stan, N.K. et al. (2015) Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.
J Cell Sci. 128 (19): 3583-96. -
Mukbel, R.M. et al. (2016) Human immune response to salivary proteins of wild-caught Phlebotomus papatasi.
Parasitol Res. 115 (9): 3345-55. -
Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Eng Des Sel. 25 (5): 251-9.
Fluorescent Spectraviewer
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra