IgG antibody

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Goat anti Human IgG F(ab')2:Alk. Phos.

Goat anti Human IgG F(ab')2:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
0500-0100
Datasheet
E WB
MSDS
0.5 ml loader

0500-0099
Datasheet
E WB
MSDS
0.5 ml loader

Goat anti Human IgG F(ab')2 antibody will recognize both the heavy and light chains of the F(ab')2 portion of human IgG by IEP. This product will also react with light chains from other human immunoglobulin types. This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.

This peroxidase-labelled antibody is a performance tested, matched reagent for HuCAL antibodies.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin
Preservative Stabilisers
0.01%Thiomersal
1%Bovine Serum Albumin
Immunogen
Human serum.
Approx. Protein Concentrations
IgG concentration 0.6 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Stable for 6 weeks at 4ºC as an undiluted liquid. For longer term storage (>6 weeks) please add an equal volume of glycerol and store at -20ºC. Avoid freeze-thaw cycles.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01857 Related reagents
P01861 Related reagents
P01834 Related reagents
P01859 Related reagents
P01860 Related reagents
P0CG04 Related reagents
Entrez Gene
IGKC Related reagents
IGHG1 Related reagents
IGHG2 Related reagents
IGHG3 Related reagents
IGHG4 Related reagents
IGLV2-14 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1,000 1/10,000
Western Blotting 1/1,000 1/10,000
ELISA 1/5,000 1/100,000
Western Blotting 1/10,000 1/200,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG antibody

  1. Wang, Y. et al. (2011) Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures.
    Mol Pharm. 8 (4): 1016-24.
  2. Ohashi, M. et al. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
    J Biol Chem. 285 (22): 16408-15.
  3. Thiede-Stan, N.K. et al. (2015) Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.
    J Cell Sci. 128 (19): 3583-96.
  4. Mukbel, R.M. et al. (2016) Human immune response to salivary proteins of wild-caught Phlebotomus papatasi.
    Parasitol Res. 115 (9): 3345-55.
  5. Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
    Protein Eng Des Sel. 25 (5): 251-9.