IgG antibody
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Goat anti Human IgG F(ab')2 antibody will recognize both the heavy and light chains of the F(ab')2 portion of human IgG by IEP. This product will also react with light chains from other human immunoglobulin types. This antibody has been cross adsorbed against the Fc domain and will react with less than 1 % of the Fc domain of human IgG.
This peroxidase-labelled antibody is a performance tested, matched reagent for HuCAL antibodies.
This peroxidase-labelled antibody is a performance tested, matched reagent for HuCAL antibodies.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% Sodium Azide (NaN3)
1.5% Bovine Serum Albumin - Preservative Stabilisers
0.01% Thiomersal 1% Bovine Serum Albumin - Immunogen
- Human serum.
- Approx. Protein Concentrations
- IgG concentration 0.6 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Stable for 6 weeks at 4ºC as an undiluted liquid. For longer term storage (>6 weeks) please add an equal volume of glycerol and store at -20ºC. Avoid freeze-thaw cycles.
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01857
- P01861
- P01834
- P01859
- P01860
- P0CG04
- Entrez Gene
- IGKC
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- IGLV2-14
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0005576 extracellular region
- GO:0006958 complement activation, classical pathway
- GO:0045087 innate immune response
- GO:0005624 membrane fraction
- GO:0006955 immune response
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1,000 | 1/10,000 | |
| Western Blotting | 1/1,000 | 1/10,000 | |
| ELISA | 1/5,000 | 1/100,000 | |
| Western Blotting | 1/10,000 | 1/200,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Wang, Y. et al. (2011) Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures.
Mol Pharm. 8 (4): 1016-24. -
Ohashi, M. et al. (2010) Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
J Biol Chem. 285 (22): 16408-15. -
Thiede-Stan, N.K. et al. (2015) Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex.
J Cell Sci. 128 (19): 3583-96. -
Mukbel, R.M. et al. (2016) Human immune response to salivary proteins of wild-caught Phlebotomus papatasi.
Parasitol Res. 115 (9): 3345-55. -
Back, J.W. et al. (2012) Selecting highly structure-specific antibodies using structured synthetic mimics of the cystine knot protein sclerostin.
Protein Eng Des Sel. 25 (5): 251-9.
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