IgA antibody
Biotinylated Goat anti Human IgA antibody used for the evaluation of human IgA responses to pneumococcal challenge by ELISA with subsequent detection by alkaline phosphatase conjugated streptavidin (STAR6B) and p-nitrophenyl phosphate.
Image caption:
IgG and IgA responses to whole cell 23F or 6B pneumococci following 23F or 6B challenge, respectively. ELISAs were performed using 23F or 6B pneumococci as targets to measure specific IgG (A, C and E) and IgA (B, D and F) titers in serum (A and B), NW (C and D) and BAL (E and F). Values shown are the mean antibody titers (of triplicates), pre and post 23F (n = 7) or 6B (n = 8) challenge (x-axis). *represents statistical significance between pre- and post-inoculation antibody titers.
From: Wright AKA, Ferreira DM, Gritzfeld JF, Wright AD, Armitage K, Jambo KC, et al.. (2012)
Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog 8(4): e1002622.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Biotin conjugated Goat anti Human IgA antibody (205008) used to evaluate virus specific IgA responses in human samples by ELISA.
Image caption:
Recovery of influenza specific nasal IgA is not affected by posting samples. Adult volunteers, had nasal samples collected by flocked swab. Samples were either processed directly from the nose or posted via the internal mail system. Having processed the samples they were analysed for H1N1 (a) or H3N2 (b) specific nasal IgA by ELISA. n = 6.
From: Tregoning JS.
Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.
Clin Exp Immunol. 2020 Feb;199 (2):109-18.
doi: 10.1111/cei.13395.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human IgA antibody recognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woof et al. 2004).
Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity.
Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Ig Fraction conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on pooled human IgA
- Antiserum Preparation
- Antisera to human IgA were raised by repeated immunisations of goats with highly purified antigen.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Human IgA.
- Approx. Protein Concentrations
- Ig concentration 0.5mg/ml
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- +4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- UniProt
- P01876
- P01877
- Entrez Gene
- IGHA1
- IGHA2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0006955 immune response
- GO:0018298 protein-chromophore linkage
- GO:0005624 membrane fraction
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| ELISA | 1/2000 | 1/4000 | |
| Flow Cytometry |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2022 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test |
Product Specific References
References for IgA antibody
-
Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog. 8: e1002622. -
Pennington, S.H. et al. (2016) Polysaccharide-specific Memory B-cells Predict Protection Against Experimental Human Pneumococcal Carriage.
Am J Respir Crit Care Med. Jul 12. [Epub ahead of print] -
Turner, P.J. et al. (2020) Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.
Clin Exp Immunol. 199 (2): 109-118. -
Cole, M.E. et al. (2021) Pre-existing influenza-specific nasal IgA or nasal viral infection does not affect live attenuated influenza vaccine immunogenicity in children.
Clin Exp Immunol. 204 (1): 125-133.
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