IgA antibody

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Goat anti Human IgA:Alk. Phos.

Goat anti Human IgA:Biotin

Goat anti Human IgA:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
205004 E 1 ml
205008 E 1 mg
205009 F 0.5 mg
Goat anti Human IgA antibody recognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woof et al. 2004).
Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Ig Fraction conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography on pooled human IgA
Antiserum Preparation
Antisera to human IgA were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Preservative Stabilisers
0.1% Sodium azide
Stabilising agent
Immunogen
Human IgA.
Approx. Protein Concentrations
Ig concentration 0.5mg/ml
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from the date of despatch.

More Information

UniProt
P01876 Related reagents
P01877 Related reagents
Entrez Gene
IGHA1 Related reagents
IGHA2 Related reagents
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
ELISA 1/2000 1/4000
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for IgA antibody

  1. Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
    PLoS Pathog. 8: e1002622.
  2. Pennington, S.H. et al. (2016) Polysaccharide-specific Memory B-cells Predict Protection Against Experimental Human Pneumococcal Carriage.
    Am J Respir Crit Care Med. Jul 12. [Epub ahead of print]