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IgA antibody

Goat anti Human IgA:Alk. Phos.

Product Type
Polyclonal Antibody
Polyclonal IgG

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Goat anti Human IgA antibody recognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woof et al. 2004).
Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity.

Target Species
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antisera to human IgA were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
50% Glycerol
Human IgA.
For research purposes only
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgA antibody

  1. Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
    PLoS Pathog. 8: e1002622.
  2. Pennington, S.H. et al. (2016) Polysaccharide-Specific Memory B Cells Predict Protection against Experimental Human Pneumococcal Carriage.
    Am J Respir Crit Care Med. 194 (12): 1523-31.
  3. Turner, P.J. et al. (2020) Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.
    Clin Exp Immunol. 199 (2): 109-118.
  4. Cole, M.E. et al. (2021) Pre-existing influenza-specific nasal IgA or nasal viral infection does not affect live attenuated influenza vaccine immunogenicity in children.
    Clin Exp Immunol. 204 (1): 125-133.

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0006955 immune response
GO:0018298 protein-chromophore linkage
GO:0005624 membrane fraction


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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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