Haptoglobin antibody
Sheep anti Human Haptoglobin antibody detects haptoglobin, also known as Hp, an acute phase plasma glycoprotein synthesized by the liver in response to infection or inflammation. Haptoglobin binds haemoglobin released by erythrocytes with high affinity, and functions to block the oxidative activity of haemoglobin and prevent renal damage. The haptoglobin-haemoglobin complex is rapidly removed from the circulation by monocytes and macrophages, via the receptor CD163.
The haptoglobin genotype has been demonstrated to be an independent risk factor for cardiovascular disease in multiple epidemiological studies.
The haptoglobin genotype has been demonstrated to be an independent risk factor for cardiovascular disease in multiple epidemiological studies.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.01% Thiomersal - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Native, purified human haptoglobin from serum.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00738
- Entrez Gene
- HP
- GO Terms
- GO:0006952 defense response
- GO:0004252 serine-type endopeptidase activity
- GO:0005576 extracellular region
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0010942 positive regulation of cell death
- GO:0030492 hemoglobin binding
- GO:0051354 negative regulation of oxidoreductase activity
- GO:2000296 negative regulation of hydrogen peroxide catabolic process
- Regulatory
- For research purposes only
Applications of Haptoglobin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/1000 | |
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Haptoglobin antibody
-
Carter, K. & Worwood, M. (2007) Haptoglobin: a review of the major allele frequencies worldwide and their association with diseases.
Int J Lab Hematol. 29 (2): 92-110. -
Guetta, J. et al. (2007) Haptoglobin genotype modulates the balance of Th1/Th2 cytokines produced by macrophages exposed to free hemoglobin.
Atherosclerosis. 191: 48-53. -
Araki, H. et al. (2015) Haptoglobin promoter polymorphism rs5472 as a prognostic biomarker for peptide vaccine efficacy in castration-resistant prostate cancer patients.
Cancer Immunol Immunother. 64 (12): 1565-73. -
Soejima, M. et al. (2014) Genetic factors associated with serum haptoglobin level in a Japanese population.
Clin Chim Acta. 433: 54-7.
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