GRP94 antibody

100% Secure


Goat anti GRP94

GRP94 antibody recognizes endoplasmin, also known as 94 kDa glucose-regulated protein or GRP-94 Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog. The antibody has been extensively validated for western blotting using whole cell lysates.

GRP94 Antibody with Control Lysate

GRP94 antibody recognizes endoplasmin, also known as 94 kDa glucose-regulated protein or GRP-94 Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00059 WB 100 µl
VPA00059KT WB 2 Westerns
With Lysate 400µg
Goat anti Human GRP94 antibody recognizes GRP94 a 94 kDa glucose regulated protein (GRP94), also known as tumor rejection antigen 1 (TRA1), heat shock protein 90 kDa beta member 1 (HSP90B1) and endoplasmin. GRP94 is an endoplasmic reticulum resident molecular chaperone, which is a paralog of cytoplasmic hsp90. GRP94 assists in the folding of membrane-bound proteins including the toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.

Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.

Product Details

Target Species
Human
Western Blotting
Goat anti GRP94 detects a band of approximately 100 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-KEGVKFDESEKTKE from the internal region of the protein sequence.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P14625 Related reagents
Entrez Gene
HSP90B1 Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0003723 RNA binding
GO:0006457 protein folding
GO:0005829 cytosol
GO:0051208 sequestering of calcium ion
GO:0005524 ATP binding
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006916 anti-apoptosis
GO:0015031 protein transport
GO:0019903 protein phosphatase binding
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031247 actin rod assembly
GO:0042470 melanosome
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0046790 virion binding
GO:0048471 perinuclear region of cytoplasm
GO:0050750 low-density lipoprotein particle receptor binding
GO:0071318 cellular response to ATP
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of GRP94 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

Contact Information


Online Payment Methods:

  1. Invoice / Purchase Order
  2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
credit cards accepted