GRP94 antibody

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Anti GRP94 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with GRP94 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to GRP94 (molecular weight 100 kDa)

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Goat anti GRP94

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: GRP94

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VPA00059 Datasheet Datasheet SDS SDS	WB	100 µl		Log in	Get Quote
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Goat anti Human GRP94 antibody recognizes GRP94 a 94 kDa glucose regulated protein (GRP94), also known as tumor rejection antigen 1 (TRA1), heat shock protein 90 kDa beta member 1 (HSP90B1) and endoplasmin. GRP94 is an endoplasmic reticulum resident molecular chaperone, which is a paralog of cytoplasmic hsp90. GRP94 assists in the folding of membrane-bound proteins including the toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.

Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.

Target Species: Human
Western Blotting: Goat anti GRP94 detects a band of approximately 100 kDa in MCF7 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide with sequence C-KEGVKFDESEKTKE from the internal region of the protein sequence.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P14625
Entrez Gene: HSP90B1
GO Terms: GO:0051082 unfolded protein binding; GO:0005509 calcium ion binding; GO:0001666 response to hypoxia; GO:0003723 RNA binding; GO:0006457 protein folding; GO:0005829 cytosol; GO:0051208 sequestering of calcium ion; GO:0005524 ATP binding; GO:0005788 endoplasmic reticulum lumen; View More GO Terms; GO:0005789 endoplasmic reticulum membrane; GO:0005792 microsome; GO:0006916 anti-apoptosis; GO:0015031 protein transport; GO:0019903 protein phosphatase binding; GO:0030433 ER-associated protein catabolic process; GO:0030496 midbody; GO:0031247 actin rod assembly; GO:0042470 melanosome; GO:0043666 regulation of phosphoprotein phosphatase activity; GO:0046790 virion binding; GO:0048471 perinuclear region of cytoplasm; GO:0050750 low-density lipoprotein particle receptor binding; GO:0071318 cellular response to ATP