GRP94 antibody
Goat anti GRP94
GRP94 antibody recognizes endoplasmin, also known as 94 kDa glucose-regulated protein or GRP-94 Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog. The antibody has been extensively validated for western blotting using whole cell lysates.
GRP94 Antibody with Control Lysate
GRP94 antibody recognizes endoplasmin, also known as 94 kDa glucose-regulated protein or GRP-94 Heat shock protein 90 kDa beta member 1 Tumor rejection antigen 1 gp96 homolog. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00059 | WB | 100 µl | ||||
| VPA00059KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human GRP94 antibody recognizes GRP94 a 94 kDa glucose regulated protein (GRP94), also known as tumor rejection antigen 1 (TRA1), heat shock protein 90 kDa beta member 1 (HSP90B1) and endoplasmin. GRP94 is an endoplasmic reticulum resident molecular chaperone, which is a paralog of cytoplasmic hsp90. GRP94 assists in the folding of membrane-bound proteins including the toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.
Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.
Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti GRP94 detects a band of approximately 100 kDa in MCF7 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence C-KEGVKFDESEKTKE from the internal region of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg MCF7 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P14625 Related reagents
- Entrez Gene
- HSP90B1 Related reagents
- GO Terms
- GO:0051082 unfolded protein binding
- GO:0005509 calcium ion binding
- GO:0001666 response to hypoxia
- GO:0003723 RNA binding
- GO:0006457 protein folding
- GO:0005829 cytosol
- GO:0051208 sequestering of calcium ion
- GO:0005524 ATP binding
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0005792 microsome
- GO:0006916 anti-apoptosis
- GO:0015031 protein transport
- GO:0019903 protein phosphatase binding
- GO:0030433 ER-associated protein catabolic process
- GO:0030496 midbody
- GO:0031247 actin rod assembly
- GO:0042470 melanosome
- GO:0043666 regulation of phosphoprotein phosphatase activity
- GO:0046790 virion binding
- GO:0048471 perinuclear region of cytoplasm
- GO:0050750 low-density lipoprotein particle receptor binding
- GO:0071318 cellular response to ATP
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of GRP94 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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