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GRP94 antibody

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Anti GRP94 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1
Anti GRP94 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with GRP94 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to GRP94 (molecular weight 100 kDa)

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Goat anti GRP94

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
GRP94

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Product Code Applications Pack Size List Price Your Price Qty
VPA00059
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SDS Safety Datasheet SDS
WB 100 µl
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Goat anti Human GRP94 antibody recognizes GRP94 a 94 kDa glucose regulated protein (GRP94), also known as tumor rejection antigen 1 (TRA1), heat shock protein 90 kDa beta member 1 (HSP90B1) and endoplasmin. GRP94 is an endoplasmic reticulum resident molecular chaperone, which is a paralog of cytoplasmic hsp90. GRP94 assists in the folding of membrane-bound proteins including the toll-like receptors, certain subclasses of integrins and some secreted proteins such as IgG.

Goat anti Human GRP94 detects GRP94 as a band of 100 kDa in a wide range of human cell line lysates tested. Cross reactivity with rodent cell lines is also observed.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Goat anti GRP94 detects a band of approximately 100 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence C-KEGVKFDESEKTKE from the internal region of the protein sequence.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P14625
Entrez Gene
HSP90B1
GO Terms
GO:0051082 unfolded protein binding
GO:0005509 calcium ion binding
GO:0001666 response to hypoxia
GO:0003723 RNA binding
GO:0006457 protein folding
GO:0005829 cytosol
GO:0051208 sequestering of calcium ion
GO:0005524 ATP binding
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0005792 microsome
GO:0006916 anti-apoptosis
GO:0015031 protein transport
GO:0019903 protein phosphatase binding
GO:0030433 ER-associated protein catabolic process
GO:0030496 midbody
GO:0031247 actin rod assembly
GO:0042470 melanosome
GO:0043666 regulation of phosphoprotein phosphatase activity
GO:0046790 virion binding
GO:0048471 perinuclear region of cytoplasm
GO:0050750 low-density lipoprotein particle receptor binding
GO:0071318 cellular response to ATP

VPA00059

166726 230914

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