Sheep anti Fibronectin
Fibronectin antibody recognizes fibronectin, also known as FN, cold-insoluble globulin or CIG. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Polyclonal IgG
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Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schilling et al. 2011).
Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed.
- Target Species
- Western Blotting
- Sheep anti Fibronectin detects a band of approxiamtely 260 kDa in A549 cell lysates.
- Species Cross-Reactivity
Target Species Cross Reactivity Mouse Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Sheep polyclonal antibody purified by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Highly pure human fibronectin.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- As supplied, 12 months from date of despatch.
- P02751 Related reagents
- Entrez Gene
- FN1 Related reagents
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- For research purposes only
Applications of Fibronectin antibody
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PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||C E P WB||1 ml|
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