E3 Ubiquitin Ligase SIAH1 antibody

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Goat anti E3 Ubiquitin Ligase SIAH1

Goat anti-human SIAH1 antibody recognizes the E3 ubiquitin-protein ligase SIAH1, also known as seven in absentia homolog 1. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00520 WB 100 µl
Goat anti Human E3 ubiquitin ligase SIAH1 antibody recognizes E3 ubiquitin-protein ligase SIAH1, also known as seven in absentia homolog 1 or siah-1a.

The SIAH1 gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis (Frank et al. 2006). Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized (provided by RefSeq, Jul 2008).

Goat anti Human E3 ubiquitin-protein ligase SIAH1 antibody detects a band of 31 kDa. The antibody has been extensively validated for western blotting using tissue and whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti E3 ubiquitin ligase SIAH1 detects a band of approximately 31 kDa in mouse brain tissue.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
Tris buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence SRQTATALPTGTSKC
Instructions For Use
Please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q8IUQ4 Related reagents
Entrez Gene
SIAH1 Related reagents
GO Terms
GO:0042787 protein ubiquitination involved in ubiquitin-dependent protein catabolic process
GO:0005829 cytosol
GO:0004842 ubiquitin-protein ligase activity
GO:0005634 nucleus
GO:0007049 cell cycle
GO:0007283 spermatogenesis
GO:0007411 axon guidance
GO:0008022 protein C-terminus binding
GO:0008270 zinc ion binding
GO:0030877 beta-catenin destruction complex
GO:0043161 proteasomal ubiquitin-dependent protein catabolic process
GO:0051402 neuron apoptosis
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only.

Applications of E3 Ubiquitin Ligase SIAH1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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