Cyclophilin A antibody

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Anti Cyclophilin A Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with cyclophilin A antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 32 second exposure. Arrow points to cyclophilin A (molecular weight 18 kDa).

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Goat anti Cyclophilin A

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Cyclophilin A

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Goat anti Human cyclophilin A antibody recognizes cyclophilin A also known as PPIase A, PPIA, T cell cyclophilin, cyclosporin A-binding protein, epididymis secretory sperm binding protein Li 69p, peptidyl-prolyl cis-trans isomerase A or rotamase A.

PPIA gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. Cyclophilin A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions (Braaten et al. 1996). Multiple pseudogenes that map to different chromosomes have been reported (provided by RefSeq, Jul 2008).

Goat anti Human cyclophilin A antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti cyclophilin A detects a band of approximately 18 kDa in A549 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid.
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin.
Immunogen: Peptide sequence C-ERFGSRNGKTSKK
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P62937
Entrez Gene: PPIA
GO Terms: GO:0051082 unfolded protein binding; GO:0002576 platelet degranulation; GO:0006457 protein folding; GO:0003755 peptidyl-prolyl cis-trans isomerase activity; GO:0005576 extracellular region; GO:0005829 cytosol; GO:0005634 nucleus; GO:0006278 RNA-dependent DNA replication; GO:0019047 provirus integration; View More GO Terms; GO:0019061 uncoating of virus; GO:0030168 platelet activation; GO:0030260 entry into host cell; GO:0042277 peptide binding; GO:0045069 regulation of viral genome replication; GO:0046790 virion binding; GO:0050900 leukocyte migration