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Cyclophilin A antibody

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Anti Cyclophilin A Antibody (PrecisionAb™ Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with cyclophilin A antibody followed by detection with Donkey anti Sheep/Goat IgG (Fc):HRP (1/10,000, STAR88P) and visualized on the ChemiDoc™ MP with 32 second exposure. Arrow points to cyclophilin A (molecular weight 18 kDa).

Goat anti Cyclophilin A

Goat anti-human cyclophilin A antibody recognizes peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, T cell cyclophilin, cyclosporin A-binding protein, epididymis secretory sperm binding protein Li 69p or rotamase A.

Cyclophilin A Antibody with Control Lysate

Goat anti-human cyclophilin A antibody recognizes peptidyl-prolyl cis-trans isomerase A, also known as PPIase A, T cell cyclophilin, cyclosporin A-binding protein, epididymis secretory sperm binding protein Li 69p or rotamase A.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00548 WB 100 µl
VPA00548KT WB 2 Westerns
With Lysate 400µg
Goat anti Human cyclophilin A antibody recognizes cyclophilin A also known as PPIase A, PPIA, T cell cyclophilin, cyclosporin A-binding protein, epididymis secretory sperm binding protein Li 69p, peptidyl-prolyl cis-trans isomerase A or rotamase A.

PPIA gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. Cyclophilin A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions (Braaten et al. 1996). Multiple pseudogenes that map to different chromosomes have been reported (provided by RefSeq, Jul 2008).

Goat anti Human cyclophilin A antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence C-ERFGSRNGKTSKK

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
P62937 Related reagents
Entrez Gene
PPIA Related reagents
GO Terms
GO:0051082 unfolded protein binding
GO:0002576 platelet degranulation
GO:0006457 protein folding
GO:0003755 peptidyl-prolyl cis-trans isomerase activity
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006278 RNA-dependent DNA replication
GO:0019047 provirus integration
GO:0019061 uncoating of virus
GO:0030168 platelet activation
GO:0030260 entry into host cell
GO:0042277 peptide binding
GO:0045069 regulation of viral genome replication
GO:0046790 virion binding
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Cyclophilin A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti cyclophilin A detects a band of approximately 18 kDa in A549 cell lysates
Western Blotting
Anti cyclophilin A detects a band of approximately 18 kDa in A549 cell lysates
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
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