Cyclophilin A antibody

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Goat anti Cyclophilin A

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Product Code Applications Pack Size List Price Quantity
VPA00548
Datasheet
WB
SDS
100 µl

Goat anti Human cyclophilin A antibody recognizes cyclophilin A also known as PPIase A, PPIA, T cell cyclophilin, cyclosporin A-binding protein, epididymis secretory sperm binding protein Li 69p, peptidyl-prolyl cis-trans isomerase A or rotamase A.

PPIA gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. Cyclophilin A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions (Braaten et al. 1996). Multiple pseudogenes that map to different chromosomes have been reported (provided by RefSeq, Jul 2008).

Goat anti Human cyclophilin A antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti cyclophilin A detects a band of approximately 18 kDa in A549 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid.
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin.
Immunogen
Peptide sequence C-ERFGSRNGKTSKK

Storage Information

Storage
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
Guarantee
12 months from date of despatch

More Information

UniProt
P62937
Entrez Gene
PPIA
GO Terms
GO:0051082 unfolded protein binding
GO:0002576 platelet degranulation
GO:0006457 protein folding
GO:0003755 peptidyl-prolyl cis-trans isomerase activity
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006278 RNA-dependent DNA replication
GO:0019047 provirus integration
GO:0019061 uncoating of virus
GO:0030168 platelet activation
GO:0030260 entry into host cell
GO:0042277 peptide binding
GO:0045069 regulation of viral genome replication
GO:0046790 virion binding
GO:0050900 leukocyte migration
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only.

Applications of Cyclophilin A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader