Ceruloplasmin antibody

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Sheep anti Human Ceruloplasmin:HRP

Sheep anti Human Ceruloplasmin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1940-0004P C E 1 ml
1940-0004 C E 1 ml
Sheep anti Human ceruloplasmin antibody detects ceruloplasmin, a ~132kDa metalloprotein that is secreted into the plasma by liver hepatocytes and activated monocytes/macrophages. Ceruloplasmin functions in copper transport, iron homeostasis, antioxidant defence, angiogenesis and coagulation.

Deficiency in ceruloplasmin is termed aceruloplasminemia, an autosomal recessive disorder which leads to iron accumulation and tissue damage, in particular in the brain and visceral organs. Clinical features include retinal degeneration, diabetes mellitus and neurological disturbances.

Elevated plasma ceruloplasmin has been shown to pose an increased risk in atherosclerosis and myocardial infarction.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Highly pure human ceruloplasmin.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00450 Related reagents
Entrez Gene
CP Related reagents
GO Terms
GO:0051087 chaperone binding
GO:0004322 ferroxidase activity
GO:0005615 extracellular space
GO:0055114 oxidation-reduction process
GO:0006825 copper ion transport
GO:0006879 cellular iron ion homeostasis
GO:0055085 transmembrane transport
Regulatory
For research purposes only

Applications of Ceruloplasmin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/5000
Immunohistology - Frozen 1/10 1/500
ELISA
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

ELISA
This antibody has been used at dilutions as high as 1/400K in a simple ELISA with 0.1 µg of antigen coated per well.
ELISA
This antibody has been used at dilutions as high as 1/400K in a simple ELISA with 0.1 µg of antigen coated per well.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Ceruloplasmin antibody

  1. Balmaña, M. et al. (2015) Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin.
    Clin Chim Acta. 442: 56-62.

Further Reading

  1. Mazumder, B. et al. (2005) Regulation of macrophage ceruloplasmin gene expression: one paradigm of 3'-UTR-mediated translational control.
    Mol Cells. 20 (2): 167-72.