Ceruloplasmin antibody
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Sheep anti Human ceruloplasmin antibody detects ceruloplasmin, a ~132kDa metalloprotein that is secreted into the plasma by liver hepatocytes and activated monocytes/macrophages. Ceruloplasmin functions in copper transport, iron homeostasis, antioxidant defence, angiogenesis and coagulation.
Deficiency in ceruloplasmin is termed aceruloplasminemia, an autosomal recessive disorder which leads to iron accumulation and tissue damage, in particular in the brain and visceral organs. Clinical features include retinal degeneration, diabetes mellitus and neurological disturbances.
Elevated plasma ceruloplasmin has been shown to pose an increased risk in atherosclerosis and myocardial infarction.
Deficiency in ceruloplasmin is termed aceruloplasminemia, an autosomal recessive disorder which leads to iron accumulation and tissue damage, in particular in the brain and visceral organs. Clinical features include retinal degeneration, diabetes mellitus and neurological disturbances.
Elevated plasma ceruloplasmin has been shown to pose an increased risk in atherosclerosis and myocardial infarction.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
HRP Stabiliser (BUF052A) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Highly pure human ceruloplasmin.
- Approx. Protein Concentrations
- Ig concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P00450
- Entrez Gene
- CP
- GO Terms
- GO:0051087 chaperone binding
- GO:0004322 ferroxidase activity
- GO:0005615 extracellular space
- GO:0055114 oxidation-reduction process
- GO:0006825 copper ion transport
- GO:0006879 cellular iron ion homeostasis
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of Ceruloplasmin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/5000 | |
| Immunohistology - Frozen | 1/10 | 1/500 | |
| ELISA | |||
| Immunohistology - Frozen |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- ELISA
- This antibody has been used at dilutions as high as 1/400K in a simple ELISA with 0.1 µg of antigen coated per well.
- ELISA
- This antibody has been used at dilutions as high as 1/400K in a simple ELISA with 0.1 µg of antigen coated per well.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for Ceruloplasmin antibody
-
Balmaña, M. et al. (2015) Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin.
Clin Chim Acta. 442: 56-62.
Further Reading
-
Mazumder, B. et al. (2005) Regulation of macrophage ceruloplasmin gene expression: one paradigm of 3'-UTR-mediated translational control.
Mol Cells. 20 (2): 167-72.
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