CD294 antibody

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Rabbit anti Human CD294 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2174 E P WB 50 µg
Rabbit anti Human CD294 antibody recognizes CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44. CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2).

CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.

PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 – KLH conjugated.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
Q9Y5Y4 Related reagents
Q9Z2J6 Related reagents
Entrez Gene
GPR44 Related reagents
Gpr44 Related reagents
GO Terms
GO:0004982 N-formyl peptide receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0005886 plasma membrane
GO:0001785 prostaglandin J receptor activity
GO:0016021 integral to membrane
GO:0004956 prostaglandin D receptor activity
GO:0004958 prostaglandin F receptor activity
GO:0006935 chemotaxis
GO:0007193 inhibition of adenylate cyclase activity by G-protein signaling pathway
GO:0019722 calcium-mediated signaling
GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
Regulatory
For research purposes only

Applications of CD294 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 5ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
*This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue
Human adrenal gland

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P