CD294 antibody
Rabbit anti Human CD294 antibody recognizes CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44. CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2).
CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.
PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.
CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.
PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 – KLH conjugated.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y5Y4
- Q9Z2J6
- Entrez Gene
- GPR44
- Gpr44
- GO Terms
- GO:0004982 N-formyl peptide receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0005886 plasma membrane
- GO:0001785 prostaglandin J receptor activity
- GO:0016021 integral to membrane
- GO:0004956 prostaglandin D receptor activity
- GO:0004958 prostaglandin F receptor activity
- GO:0006935 chemotaxis
- GO:0007193 inhibition of adenylate cyclase activity by G-protein signaling pathway
- GO:0019722 calcium-mediated signaling
- GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
- Regulatory
- For research purposes only
Applications of CD294 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin | 5ug/ml | ||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Immunohistology
- *This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose. - Histology Positive Control Tissue
- Human adrenal gland
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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