CD294 antibody




CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.
PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 – KLH conjugated.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9Y5Y4
- Q9Z2J6
- Entrez Gene
- GPR44
- Gpr44
- GO Terms
- GO:0004982 N-formyl peptide receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0005886 plasma membrane
- GO:0001785 prostaglandin J receptor activity
- GO:0016021 integral to membrane
- GO:0004956 prostaglandin D receptor activity
- GO:0004958 prostaglandin F receptor activity
- GO:0006935 chemotaxis
- GO:0007193 inhibition of adenylate cyclase activity by G-protein signaling pathway
- GO:0019722 calcium-mediated signaling
- GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
- Regulatory
- For research purposes only
Applications of CD294 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | |||
Immunohistology - Paraffin | 5ug/ml | ||
Western Blotting |
- Immunohistology
- *This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose. - Histology Positive Control Tissue
- Human adrenal gland
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | ||
Histar Detection System | STAR3000A | C P | 50 Tests |
Fluorescent Spectraviewer
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- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra