CD294 antibody

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Anti Human CD294 (N-Terminal) Antibody thumbnail image 1

Anti Human CD294 (N-Terminal) Antibody thumbnail image 2

Anti Human CD294 (N-Terminal) Antibody thumbnail image 3

Anti Human CD294 (N-Terminal) Antibody thumbnail image 4

Anti Human CD294 (N-Terminal) Antibody gallery image 1 — Staining of formalin fixed, paraffin embedded human brain cortex with Rabbit anti Human CD294 (**AHP2174**)

Anti Human CD294 (N-Terminal) Antibody gallery image 2 — Staining of formalin fixed, paraffin embedded human adrenal gland with Rabbit anti Human CD294 (**AHP2174**)

Anti Human CD294 (N-Terminal) Antibody gallery image 3 — Staining of formalin fixed, paraffin embedded human cerebellum with Rabbit anti Human CD294 (**AHP2174**)

Anti Human CD294 (N-Terminal) Antibody gallery image 4 — Western blotting of Jurkat cell lysate probed with Rabbit anti Human CD294 (**AHP2174**)

Rabbit anti Human CD294 (N-Terminal)

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
AHP2174	E P WB			50 µg

Rabbit anti Human CD294 antibody recognizes CD294, also known as chemoattractant receptor homologous molecule expressed on Th2 cells (CRTH2) and GRP44. CD294 is a G protein-coupled receptor which has a high affinity for prostaglandin D2 (PGD2).

CD294 is predominantly expressed on Th2 cells, basophils and eosinophils, and its activation by PDG2 mediates chemotaxis of these cells to inflammatory sites. CD294 may be a useful marker for Th2 cells, and enhances their function and signalling with a possible role in Th2 cell differentiation.

PGD2 is thought to contribute to bronchoconstriction, eosinophilia and mucus production in allergic asthma, and CD294 may contribute to this disease.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse	Expected from Sequence
Rat	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Antiserum Preparation

Antisera to human CD294 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.02% Sodium Azide (NaN₃)

Immunogen

A synthetic peptide corresponding to an 18 amino acid sequence from near the amino terminus of human CD294 – KLH conjugated.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.

More Information

UniProt: Q9Y5Y4 Related reagents; Q9Z2J6 Related reagents
Entrez Gene: GPR44 Related reagents; Gpr44 Related reagents
GO Terms: GO:0004982 N-formyl peptide receptor activity; GO:0005887 integral to plasma membrane; GO:0006955 immune response; GO:0005886 plasma membrane; GO:0001785 prostaglandin J receptor activity; GO:0016021 integral to membrane; GO:0004956 prostaglandin D receptor activity; GO:0004958 prostaglandin F receptor activity; GO:0006935 chemotaxis; GO:0007193 inhibition of adenylate cyclase activity by G-protein signaling pathway; GO:0019722 calcium-mediated signaling; GO:0045745 positive regulation of G-protein coupled receptor protein signaling pathway
Regulatory: For research purposes only

Applications of CD294 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Paraffin		5ug/ml
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Immunohistology: *This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

Sodium citrate buffer pH 6.0 is recommended for this purpose.
Histology Positive Control Tissue: Human adrenal gland

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Sheep anti Rabbit IgG:FITC	STAR34B	1 mg	C F
Sheep anti Rabbit IgG:RPE	STAR35A	1 ml	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Antigen Retrieval Buffer, pH8.0	BUF025A	500 ml	P
Antigen Retrieval Buffer, pH8.0	BUF025C	50 ml	P
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*
Histar Detection System	STAR3000A	50 Tests	C P

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