CD20 antibody

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Goat anti CD20

CD20 (c terminus) antibody recognizes B lymphocyte antigen CD20, also known as B lymphocyte surface antigen B1 Bp35 leukocyte surface antigen Leu-16, membrane-spanning 4-domains subfamily, a member 1 CD20. The antibody has been extensively validated for western blotting using whole cell lysates.

CD20 Antibody with Control Lysate

CD20 (c terminus) antibody recognizes B lymphocyte antigen CD20, also known as B lymphocyte surface antigen B1 Bp35 leukocyte surface antigen Leu-16, membrane-spanning 4-domains subfamily, a member 1 CD20. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00099 WB 100 µl
VPA00099KT WB 2 Westerns
With Lysate 400µg
Goat anti Human CD20 antibody recognizes the B-lymphocyte antigen CD20, also known as B-lymphocyte surface antigen B1, Bp35, leukocyte surface antigen Leu-16 and membrane-spanning 4-domains subfamily A member 1.

CD20, encoded by the MS4A1 gene, is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 is a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of MS4A1 results in two transcript variants which encode the same protein (provided by RefSeq, Jul 2008).

Goat anti Human CD20 antibody detects a band of 33 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Preparation
20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution
TRIS buffered saline
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5 % BSA
Immunogen
Peptide with the sequence CQDQESSPIENDSSP from the C terminus of the protein.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P11836 Related reagents
Entrez Gene
MS4A1 Related reagents
GO Terms
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0042113 B cell activation
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of CD20 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Western Blotting
Anti CD20 detects a band of approximately 33 kDa in Raji cell lysates.
Western Blotting
Anti CD20 detects a band of approximately 33 kDa in Raji cell lysates.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

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