Carbonic Anhydrase II antibody

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Sheep anti Carbonic Anhydrase II

Carbonic anhydrase II antibody recognizes carbonic anhydrase 2, also known as carbonate dehydratase II, carbonic anhydrase C, CAC, carbonic anhydrase II or CA-II. The antibody has been extensively validated for western blotting using whole cell lysates.

Carbonic Anhydrase II Antibody with Control Lysate

Carbonic anhydrase II antibody recognizes carbonic anhydrase 2, also known as carbonate dehydratase II, carbonic anhydrase C, CAC, carbonic anhydrase II or CA-II. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00073 WB 100 µl
VPA00073KT WB 2 Westerns
With Lysate 400µg
Sheep anti Human carbonic anhydrase II antibody recognizes carbonic anhydrase II (CA-II), also known as carbonate dehydratase II and carbonic anhydrase C (CAC). CA-II is a ubiquitous zinc enzyme that catalyzes the reversible conversion of carbonic acid (H2CO3) to carbon dioxide (CO2) and water (H2O). Carbonic anhydrase inhibitors have now been a mainstay of human clinical intervention for several decades, with at least 25 clinically used drugs that are CA inhibitors

Sheep anti Human carbonic anhydrase II antibody detects a single band of 29 kDa in the human epidermoid carcinoma A431 cell line lysate. Cross reactivity with mouse cell lines is also seen.

Product Details

Target Species
Human
Western Blotting
Sheep anti carbonic anhydrase II detects a band of approximately 29 kDa in A431 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Sheep polyclonal antibody purified by affinity chromatography
Preparation
20μl Sheep polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg A431 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P00918 Related reagents
Entrez Gene
CA2 Related reagents
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
GO:0045177 apical part of cell
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Carbonic Anhydrase II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):HRP 5184-2504 2 mg C E WB
Rabbit anti Sheep IgG (H/L):HRP 5184-2504 2 mg C E WB

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