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Carbonic Anhydrase II antibody

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Anti Human Carbonic Anhydrase II Antibody thumbnail image 1 Anti Human Carbonic Anhydrase II Antibody thumbnail image 2 Anti Human Carbonic Anhydrase II Antibody thumbnail image 3
Anti Human Carbonic Anhydrase II Antibody gallery image 1
Published customer image:
Sheep anti Human carbonic anhydrase antibody (AHP206) used for the identification of oligodendrocytes in mouse tissues by immunofluorescence.
Image caption:
TGFβ signaling in neurons, oligodendrocytes and endothelial cells is unchanged by stroke. A.) Co-localization of pSmad2 (marker of TGFβ signaling) with Millimark Pan-Neuronal antibody cocktail (marker of neurons). B.) Co-localization of pSmad2 with CAII (oligodendrocyte marker). C.) Co-localization of pSmad2 with β-dystroglycan (endothelial cell marker). Images (40×) are from young females at day 3 post stroke but are representative of all time points for both young and old, male and female mice.

From: Doyle KP, Cekanaviciute E, Mamer LE, Buckwalter MS.
TGFβ signaling in the brain increases with aging and signals to astrocytes and innate immune cells in the weeks after stroke.
J Neuroinflammation. 2010 Oct 11;7:62.
Anti Human Carbonic Anhydrase II Antibody gallery image 2
Published customer image:
Sheep anti Human Carbonic Anhydrase II antibody (AHP206) used to evaluate CAII expression in rat brain sections by immunofluorescence.
Image caption:
Markers for immature and mature oligodendrocytes (OLs) and myelin selected for immunohistochemical analysis: GSTπ, glutathione S-transferase pi; CAII, carbonic anhydrase II; MBP, myelin basic protein. B Representative GSTπ, CAII, and MBP staining. Scale bar = 20 μm. DAPI, 4′,6-diamidino-2-phenylindole. C Example coronal section with hippocampal, amygdala, and corpus callosum ROIs indicated in red. Scale bar = 2000 μm.

From: Long KLP, Chao LL, Kazama Y, An A, Hu KY, Peretz L, Muller DCY, Roan VD, Misra R, Toth CE, Breton JM, Casazza W, Mostafavi S, Huber BR, Woodward SH, Neylan TC, Kaufer D.
Regional gray matter oligodendrocyte- and myelin-related measures are associated with differential susceptibility to stress-induced behavior in rats and humans.
Transl Psychiatry. 2021 Dec 13;11(1):631.
doi: 10.1038/s41398-021-01745-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Human Carbonic Anhydrase II Antibody gallery image 3
Published customer image:
Sheep anti Human Carbonic Anhydrase II antibody (AHP206) used to evaluate CAII expression in rat brain sections by immunofluorescence.
Image caption:
D–I Representative images of each oligodendrocyte marker from the dentate gyrus of animals with low (score = 2) or high (score = 19) composite behavior scores. Scale bar = 100 μm. J Dentate gyrus GSTπ measures from animals with low (2–6) and high (7 or more) composite behavior scores. All data are presented as mean ± SEM. Granule cell layer (GCL): low = 35.15 ± 2.64 cells/mm2, high = 49.16 ± 3.46 cells/mm2; two-tailed t test, t(9) = 3.11, p = 0.013. Hilus: low = 139.20 ± 14.40 cells/mm2, high = 178.30 ± 28.69 cells/mm2; two-tailed t test, t(9) = 1.14, p = 0.28. K CAII measures. GCL: low = 33.03 ± 3.75 cells/mm2, high = 38.57 ± 2.32 cells/mm2; two-tailed t test, t(8) = −1.26, p = 0.24, one high outlier removed. Hilus: low = 129.50 ± 22.95 cells/mm2, high = 164.40 ± 33.82 cells/mm2; two-tailed t test, t(9) = 0.82, p = 0.43. L MBP measures. GCL: low = 1366 ± 91 integrated fluorescence intensity/μm2, high = 1699 ± 74 integrated fluorescence intensity/μm2; two-tailed t test, t(9) = 2.86, p = 0.019. Hilus: low = 2496 ± 150 integrated fluorescence intensity/μm2, high = 3136 ± 142 integrated fluorescence intensity/μm2; two-tailed t test, t(9) = 3.09, p = 0.013.

From: Long KLP, Chao LL, Kazama Y, An A, Hu KY, Peretz L, Muller DCY, Roan VD, Misra R, Toth CE, Breton JM, Casazza W, Mostafavi S, Huber BR, Woodward SH, Neylan TC, Kaufer D.
Regional gray matter oligodendrocyte- and myelin-related measures are associated with differential susceptibility to stress-induced behavior in rats and humans.
Transl Psychiatry. 2021 Dec 13;11(1):631.
doi: 10.1038/s41398-021-01745-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Sheep anti Human Carbonic Anhydrase II

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Carbonic Anhydrase II

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AHP206
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Sheep anti Human carbonic anhydrase II antibody recognizes human carbonic anhydrase II, also known as Carbonate dehydratase II or Carbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.

Mutations in the CA2 gene has been identified as the cause of osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidosis (Shah et al 2004).

Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.1% EACA
0.01% Benzamidine
1 mM EDTA
Immunogen
Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion
Immunofluorescence
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Western Blotting
Sheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, see Salman et al. for details.

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References for Carbonic Anhydrase II antibody

  1. Toye, A.M. et al. (2002) Band 3 Walton, a C-terminal deletion associated with distal renal tubular acidosis, is expressed in the red cell membrane but retained internally in kidney cells.
    Blood. 99 (1): 342-7.
  2. Garcia, A.D. et al. (2010) Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain.
    J Neurosci. 30: 13597-608.
  3. Doyle, K.P. et al.. (2010) TGFβ signaling in the brain increases with aging and signals to astrocytes and innate immune cells in the weeks after stroke.
    J Neuroinflammation. 7: 62.
  4. Alvarez, B.V. et al. (2005) Metabolon disruption: a mechanism that regulates bicarbonate transport.
    EMBO J. 24: 2499-511.
  5. Tzartos, J.S. et al. (2008) Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis.
    Am J Pathol. 172: 146-55.
  6. Sudarov, A. et al. (2011) Ascl1 genetics reveals insights into cerebellum local circuit assembly.
    J Neurosci. 31: 11055-69.
  7. Salman, S. et al. (2013) Chronic exposure of neonatal rat adrenomedullary chromaffin cells to opioids in vitro blunts both hypoxia and hypercapnia chemosensitivity.
    J Physiol. 591 (Pt 2): 515-29.
  8. Salman, S. et al. (2014) Chronic opioids regulate KATP channel subunit Kir6.2 and carbonic anhydrase I and II expression in rat adrenal chromaffin cells via HIF-2α and protein kinase A.
    Am J Physiol Cell Physiol. 307: C266-77.
    9. View The Latest Product References
  9. Gailly, P. et al. (2008) A novel renal carbonic anhydrase type III plays a role in proximal tubule dysfunction.
    Kidney Int. 74 (1): 52-61.
  10. Sterling, D. et al. (2002) The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II.
    Am J Physiol Cell Physiol. 283 (5): C1522-9.
  11. Alvarez, L. et al. (2001) Carbonic anhydrase II associated with plasma membrane in a human pancreatic duct cell line (CAPAN-1).
    J Histochem Cytochem. 49 (8): 1045-53.
  12. Alvarez, B.V. et al. (2003) Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter.
    Biochemistry. 42 (42): 12321-9.
  13. Sterling, D. et al. (2002) The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV.
    J Biol Chem. 277 (28): 25239-46.
  14. Long, K.L.P. et al. (2021) Regional gray matter oligodendrocyte- and myelin-related measures are associated with differential susceptibility to stress-induced behavior in rats and humans.
    Transl Psychiatry. 11 (1): 631.

Immunofluorescence

Western Blotting

Synonyms
CA2
RRID
AB_321215
UniProt
P00918
Entrez Gene
CA2
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
GO:0045177 apical part of cell
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