Carbonic Anhydrase II antibody

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Sheep anti Human Carbonic Anhydrase II

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP206 ID IF WB 1 ml
Sheep anti Human carbonic anhydrase II antibody recognizes human carbonic anhydrase II, also known as Carbonate dehydratase II or Carbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.

Mutations in the CA2 gene has been identified as the cause of osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidosis (Shah et al 2004).

Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Glycine buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.1%EACA
1mMEDTA
0.01%Benzamidine
Immunogen
Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P00918 Related reagents
Entrez Gene
CA2 Related reagents
GO Terms
GO:0004089 carbonate dehydratase activity
GO:0006730 one-carbon metabolic process
GO:0008270 zinc ion binding
GO:0045177 apical part of cell
Regulatory
For research purposes only

Applications of Carbonic Anhydrase II antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunodiffusion
Immunofluorescence
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Western Blotting
Sheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, see Salman et al. for details.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Carbonic Anhydrase II antibody

  1. Toye, A.M. et al. (2002) Band 3 Walton, a C-terminal deletion associated with distal renal tubular acidosis, is expressed in the red cell membrane but retained internally in kidney cells.
    Blood. 99 (1): 342-7.
  2. Garcia, A.D. et al. (2010) Sonic hedgehog regulates discrete populations of astrocytes in the adult mouse forebrain.
    J Neurosci. 30: 13597-608.
  3. Doyle, K.P. et al.. (2010) TGFβ signaling in the brain increases with aging and signals to astrocytes and innate immune cells in the weeks after stroke.
    J Neuroinflammation. 7: 62.
  4. Alvarez, B.V. et al. (2005) Metabolon disruption: a mechanism that regulates bicarbonate transport.
    EMBO J. 24: 2499-511.
  5. Tzartos, J.S. et al. (2008) Interleukin-17 production in central nervous system-infiltrating T cells and glial cells is associated with active disease in multiple sclerosis.
    Am J Pathol. 172: 146-55.
  6. Sudarov, A. et al. (2011) Ascl1 genetics reveals insights into cerebellum local circuit assembly.
    J Neurosci. 31: 11055-69.
  7. Salman, S. et al. (2013) Chronic exposure of neonatal rat adrenomedullary chromaffin cells to opioids in vitro blunts both hypoxia and hypercapnia chemosensitivity.
    J Physiol. 591 (Pt 2): 515-29.
  8. Salman, S. et al. (2014) Chronic opioids regulate KATP channel subunit Kir6.2 and carbonic anhydrase I and II expression in rat adrenal chromaffin cells via HIF-2α and protein kinase A.
    Am J Physiol Cell Physiol. 307: C266-77.
  9. Gailly, P. et al. (2008) A novel renal carbonic anhydrase type III plays a role in proximal tubule dysfunction.
    Kidney Int. 74 (1): 52-61.
  10. Sterling, D. et al. (2002) The functional and physical relationship between the DRA bicarbonate transporter and carbonic anhydrase II.
    Am J Physiol Cell Physiol. 283 (5): C1522-9.
  11. Alvarez, L. et al. (2001) Carbonic anhydrase II associated with plasma membrane in a human pancreatic duct cell line (CAPAN-1).
    J Histochem Cytochem. 49 (8): 1045-53.
  12. Alvarez, B.V. et al. (2003) Direct extracellular interaction between carbonic anhydrase IV and the human NBC1 sodium/bicarbonate co-transporter.
    Biochemistry. 42 (42): 12321-9.
  13. Sterling, D. et al. (2002) The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV.
    J Biol Chem. 277 (28): 25239-46.