Calnexin antibody

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Anti Calnexin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1

Anti Calnexin Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2

Anti Calnexin Antibody (PrecisionAb Polyclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with Goat anti Human calnexin antibody (**VPA00096**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 16 second exposure. Arrow points to calnexin (molecular weight 90 kDa).

Anti Calnexin Antibody (PrecisionAb Polyclonal Antibody) gallery image 2 — Immunoprecipitation of Calnexin from MCF-7 whole cell lysate. The Input lane is 10% input; the CALNEXIN-IP lane is the immunoprecipitation with the Calnexin antibody (2.5 µg, **VPA00096**); the IgG-IP lane is the immunoprecipitation with Goat polyclonal IgG(2.5 µg, PCP001). Western blot analysis was performed using Calnexin antibody (1/500, **VPA00096**) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP. Arrow points to Calnexin.

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Goat anti Calnexin

Product Type: PrecisionAb Polyclonal
Isotype: Polyclonal IgG
Format: Purified
Specificity: Calnexin

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Product Code Applications Pack Size List Price Your Price Qty

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VPA00096 Datasheet Datasheet SDS SDS	IP WB	100 µl		Log in	Get Quote
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Goat anti Human calnexin antibody recognizes calnexin, also known as IP90, major histocompatibility complex class I antigen-binding protein p88 or p90.

Calnexin, encoded by the CANX gene, is a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described (provided by RefSeq, Jul 2008).

Goat anti Human calnexin antibody detects a band of 90 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Target Species: Human
Western Blotting: Anti Calnexin detects a band of approximately 90 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution: TRIS buffered saline.
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃) 0.5 % BSA
Immunogen: Peptide with the sequence C-SKTPELNLDQFHDKT from the internal region of the protein.
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml.
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunoprecipitation
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Donkey anti Sheep/Goat IgG:HRP	STAR88P	C E P WB	1 ml	Log in	Get Quote
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Description	Donkey anti Sheep/Goat IgG:HRP

UniProt: P27824
Entrez Gene: CANX
GO Terms: GO:0051082 unfolded protein binding; GO:0005509 calcium ion binding; GO:0006457 protein folding; GO:0016021 integral to membrane; GO:0005529 sugar binding; GO:0005788 endoplasmic reticulum lumen; GO:0005789 endoplasmic reticulum membrane; GO:0009306 protein secretion; GO:0018279 protein N-linked glycosylation via asparagine; View More GO Terms; GO:0042470 melanosome; GO:0043687 post-translational protein modification